LOCUS       Untitled_Sequence        3783 bp    DNA     circular SYN 28-FEB-2023
FEATURES             Location/Qualifiers
     primer_bind     1012..1028
                     /label="M13 fwd"
                     /color="#a020f0"
     primer_bind     223..239
                     /label="M13 rev"
                     /color="#a020f0"
     protein_bind    199..215
                     /label="lac operator"
                     /color="#31849b"
     protein_bind    3636..3657
                     /label="CAP binding site"
                     /color="#31849b"
     protein_bind    125..146
                     /label="CAP binding site"
                     /color="#31849b"
     promoter        185..191
                     /label="lac promoter"
                     /color="#ffffff"
     terminator      2430..2469
                     /label="fd terminator"
                     /color="#ffffff"
     terminator      3691..3777
                     /label="rrnB T1 terminator"
                     /color="#ffffff"
     promoter        1474..1565
                     /label="AmpR promoter"
                     /color="#ffffff"
     terminator      1307..1401
                     /label="lambda t0 terminator"
                     /color="#ffffff"
     oriT            2616..2724
                     /label="oriT"
                     /color="#a6acb3"
     rep_origin      2760..3348
                     /label="ori"
                     /color="#ffff00"
     CDS             298..978
                     /label="mRFP1"
                     /color="#ff0000"
     CDS             1635..2426
                     /label="AmpR"
                     /color="#ccffcc"
     misc_feature    297..300
                     /label="D"
                     /color="#00ffff"
     misc_feature    982..985
                     /label="G"
                     /color="#00ffff"
ORIGIN      
        1 TTAATTAAGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA
       61 TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG
      121 CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG
      181 CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC AGCTATGACC
      241 ATGATTACGC CAAGCTTGCA TGCCTGCAGG TCGACTCTAG AGGATCCCCG GTCTCAAATG
      301 ATGGCTTCCT CCGAGGATGT TATCAAAGAG TTCATGCGTT TCAAAGTTCG TATGGAAGGT
      361 TCCGTTAACG GTCACGAGTT CGAAATCGAA GGTGAAGGTG AAGGTCGTCC GTACGAAGGT
      421 ACCCAGACCG CTAAACTGAA AGTTACCAAA GGTGGTCCGC TGCCGTTCGC TTGGGACATC
      481 CTGTCCCCGC AGTTCCAGTA CGGTTCCAAA GCTTACGTTA AACACCCGGC TGACATCCCG
      541 GACTACCTGA AACTGTCCTT CCCGGAAGGT TTCAAATGGG AACGTGTTAT GAACTTCGAA
      601 GATGGTGGTG TTGTTACCGT TACCCAGGAC TCCTCCCTGC AAGACGGTGA GTTCATCTAC
      661 AAAGTTAAAC TGCGTGGTAC CAACTTCCCG TCCGACGGTC CGGTTATGCA GAAAAAAACC
      721 ATGGGTTGGG AAGCTTCCAC CGAACGTATG TACCCGGAGG ATGGTGCTCT GAAAGGTGAA
      781 ATCAAAATGC GTCTGAAACT GAAAGACGGT GGTCACTACG ACGCTGAAGT TAAAACCACC
      841 TACATGGCTA AAAAACCGGT TCAGCTGCCG GGTGCTTACA AAACCGACAT CAAACTGGAC
      901 ATCACCTCCC ACAACGAGGA CTACACCATC GTTGAACAGT ACGAACGTGC TGAAGGTCGT
      961 CACTCCACCG GTGCTTAATA AGCTTAGAGA CCGGGTACCG AGCTCGAATT CACTGGCCGT
     1021 CGTTTTACAA CGTCGTGACT GGGAAAACCC TGGCGTTACC CAACTTAATC GCCTTGCAGC
     1081 ACATCCCCCT TTCGCCAGCT GGCGTAATAG CGAAGAGGCC CGCACCGATC GCCCTTCCCA
     1141 ACAGTTGCGC AGCCTGAATG GCGAATGGCG CCTGATGCGG TATTTTCTCC TTACGCATCT
     1201 GTGCGGTATT TCACACCGCA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA
     1261 GTTAAGCCAG CCCCGACACC CGCCAACACC CGCTGAACTA GTCTTGGACT CCTGTTGATA
     1321 GATCCAGTAA TGACCTCAGA ACTCCATCTG GATTTGTTCA GAACGCTCGG TTGCCGCCGG
     1381 GCGTTTTTTA TTGGTGAGAA TCCAGGGGTC CCCAATAATT ACGATTTAAA TTAGTAGCCC
     1441 GCCTAATGAG CGGGCTTTTT TTTAATTCCC CTATTTGTTT ATTTTTCTAA ATACATTCAA
     1501 ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA
     1561 AGAGTATGAG CATTCAGCAT TTTCGTGTGG CGCTGATTCC GTTTTTTGCG GCGTTTTGCC
     1621 TGCCGGTGTT TGCGCATCCG GAAACCCTGG TGAAAGTGAA AGATGCGGAA GATCAACTGG
     1681 GTGCGCGCGT GGGCTATATT GAACTGGATC TGAACAGCGG CAAAATTCTG GAATCTTTTC
     1741 GTCCGGAAGA ACGTTTTCCG ATGATGAGCA CCTTTAAAGT GCTGCTGTGC GGTGCGGTTC
     1801 TGAGCCGTGT GGATGCGGGC CAGGAACAAC TGGGCCGTCG TATTCATTAT AGCCAGAACG
     1861 ATCTGGTGGA ATATAGCCCG GTGACCGAAA AACATCTGAC CGATGGCATG ACCGTGCGTG
     1921 AACTGTGCAG CGCGGCGATT ACCATGAGCG ATAACACCGC GGCGAACCTG CTGCTGACGA
     1981 CCATTGGCGG TCCGAAAGAA CTGACCGCGT TTCTGCATAA CATGGGCGAT CATGTGACCC
     2041 GTCTGGATCG TTGGGAACCG GAACTGAACG AAGCGATTCC GAACGATGAA CGTGATACCA
     2101 CCATGCCGGC AGCAATGGCG ACCACCCTGC GTAAACTGCT GACGGGTGAG CTGCTGACCC
     2161 TGGCAAGCCG CCAGCAACTG ATTGATTGGA TGGAAGCGGA TAAAGTGGCG GGTCCGCTGC
     2221 TGCGTAGCGC GCTGCCGGCT GGCTGGTTTA TTGCGGATAA AAGCGGTGCG GGCGAACGTG
     2281 GCAGCCGTGG CATTATTGCG GCGCTGGGCC CGGATGGTAA ACCGAGCCGT ATTGTGGTGA
     2341 TTTATACCAC CGGCAGCCAG GCGACGATGG ATGAACGTAA CCGTCAGATT GCGGAAATTG
     2401 GCGCGAGCCT GATTAAACAT TGGTAAACCG ATACAATTAA AGGCTCCTTT TGGAGCCTTT
     2461 TTTTTTGGAC GACCCTTGTC CTTTTCCGCT GCATAACCCT GCTTCGGGGT CATTATAGCG
     2521 ATTTTTTCGG TATATCCATC CTTTTTCGCA CGATATACAG GATTTTGCCA AAGGGTTCGT
     2581 GTAGACTTTC CTTGGTGTAT CCAACGGCGT CAGCCGGGCA GGATAGGTGA AGTAGGCCCA
     2641 CCCGCGAGCG GGTGTTCCTT CTTCACTGTC CCTTATTCGC ACCTGGCGGT GCTCAACGGG
     2701 AATCCTGCTC TGCGAGGCTG GCCGTAGGCC GGCCCCGTAG AAAAGATCAA AGGATCTTCT
     2761 TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA
     2821 GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC
     2881 AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC
     2941 AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT
     3001 GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG
     3061 GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC
     3121 TACACCGAAC TGAGATACCT ACAGCGTGAG CTTTGAGAAA GCGCCACGCT TCCCGAAGGG
     3181 AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG
     3241 CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT
     3301 GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC
     3361 GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG
     3421 TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC
     3481 CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA
     3541 CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT
     3601 CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG
     3661 GCAGGCGCGC CCAGCTGTCT AGGGCGGCGG ATTTGTCCTA CTCAGGAGAG CGTTCACCGA
     3721 CAAACAACAG ATAAAACGAA AGGCCCAGTC TTTCGACTGA GCCTTTCGTT TTATTTGATG
     3781 CCT     
//
