LOCUS       Untitled_Sequence        3819 bp    DNA     circular SYN 28-FEB-2023
FEATURES             Location/Qualifiers
     primer_bind     1048..1064
                     /label="M13 fwd"
                     /color="#a020f0"
     primer_bind     223..239
                     /label="M13 rev"
                     /color="#a020f0"
     protein_bind    199..215
                     /label="lac operator"
                     /color="#31849b"
     protein_bind    3672..3693
                     /label="CAP binding site"
                     /color="#31849b"
     protein_bind    125..146
                     /label="CAP binding site"
                     /color="#31849b"
     promoter        185..191
                     /label="lac promoter"
                     /color="#ffffff"
     terminator      2466..2505
                     /label="fd terminator"
                     /color="#ffffff"
     terminator      3727..3813
                     /label="rrnB T1 terminator"
                     /color="#ffffff"
     promoter        1510..1601
                     /label="AmpR promoter"
                     /color="#ffffff"
     terminator      1343..1437
                     /label="lambda t0 terminator"
                     /color="#ffffff"
     oriT            2652..2760
                     /label="oriT"
                     /color="#a6acb3"
     rep_origin      2796..3384
                     /label="ori"
                     /color="#ffff00"
     CDS             298..1017
                     /label="GFP"
                     /color="#05fd14"
     CDS             1671..2462
                     /label="AmpR"
                     /color="#ccffcc"
     misc_feature    1018..1021
                     /label="G"
                     /color="#00ffff"
     misc_feature    297..300
                     /label="D"
                     /color="#00ffff"
ORIGIN      
        1 TTAATTAAGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA
       61 TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG
      121 CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG
      181 CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC AGCTATGACC
      241 ATGATTACGC CAAGCTTGCA TGCCTGCAGG TCGACTCTAG AGGATCCCCG GTCTCAAATG
      301 ATGCGTAAAG GAGAAGAACT TTTCACTGGA GTTGTCCCAA TTCTTGTTGA ATTAGATGGT
      361 GATGTTAATG GGCACAAATT TTCTGTCAGT GGAGAGGGTG AAGGTGATGC AACATACGGA
      421 AAACTTACCC TTAAATTTAT TTGCACTACT GGAAAACTAC CTGTTCCATG GCCAACACTT
      481 GTCACTACTT TCGGTTATGG TGTTCAATGC TTTGCGAGAT ACCCAGATCA TATGAAACAG
      541 CATGACTTTT TCAAGAGTGC CATGCCCGAA GGTTATGTAC AGGAAAGAAC TATATTTTTC
      601 AAAGATGACG GGAACTACAA GACACGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT
      661 AATAGAATCG AGTTAAAAGG TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA
      721 TTGGAATACA ACTATAACTC ACACAATGTA TACATCATGG CAGACAAACA AAAGAATGGA
      781 ATCAAAGTTA ACTTCAAAAT TAGACACAAC ATTGAAGATG GAAGCGTTCA ACTAGCAGAC
      841 CATTATCAAC AAAATACTCC AATTGGCGAT GGCCCTGTCC TTTTACCAGA CAACCATTAC
      901 CTGTCCACAC AATCTGCCCT TTCGAAAGAT CCCAACGAAA AGAGAGATCA CATGGTCCTT
      961 CTTGAGTTTG TAACAGCTGC TGGGATTACA CATGGCATGG ATGAACTATA CAAATAAGCT
     1021 TAGAGACCGG GTACCGAGCT CGAATTCACT GGCCGTCGTT TTACAACGTC GTGACTGGGA
     1081 AAACCCTGGC GTTACCCAAC TTAATCGCCT TGCAGCACAT CCCCCTTTCG CCAGCTGGCG
     1141 TAATAGCGAA GAGGCCCGCA CCGATCGCCC TTCCCAACAG TTGCGCAGCC TGAATGGCGA
     1201 ATGGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATG
     1261 GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGCCCC GACACCCGCC
     1321 AACACCCGCT GAACTAGTCT TGGACTCCTG TTGATAGATC CAGTAATGAC CTCAGAACTC
     1381 CATCTGGATT TGTTCAGAAC GCTCGGTTGC CGCCGGGCGT TTTTTATTGG TGAGAATCCA
     1441 GGGGTCCCCA ATAATTACGA TTTAAATTAG TAGCCCGCCT AATGAGCGGG CTTTTTTTTA
     1501 ATTCCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT
     1561 AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGCATT CAGCATTTTC
     1621 GTGTGGCGCT GATTCCGTTT TTTGCGGCGT TTTGCCTGCC GGTGTTTGCG CATCCGGAAA
     1681 CCCTGGTGAA AGTGAAAGAT GCGGAAGATC AACTGGGTGC GCGCGTGGGC TATATTGAAC
     1741 TGGATCTGAA CAGCGGCAAA ATTCTGGAAT CTTTTCGTCC GGAAGAACGT TTTCCGATGA
     1801 TGAGCACCTT TAAAGTGCTG CTGTGCGGTG CGGTTCTGAG CCGTGTGGAT GCGGGCCAGG
     1861 AACAACTGGG CCGTCGTATT CATTATAGCC AGAACGATCT GGTGGAATAT AGCCCGGTGA
     1921 CCGAAAAACA TCTGACCGAT GGCATGACCG TGCGTGAACT GTGCAGCGCG GCGATTACCA
     1981 TGAGCGATAA CACCGCGGCG AACCTGCTGC TGACGACCAT TGGCGGTCCG AAAGAACTGA
     2041 CCGCGTTTCT GCATAACATG GGCGATCATG TGACCCGTCT GGATCGTTGG GAACCGGAAC
     2101 TGAACGAAGC GATTCCGAAC GATGAACGTG ATACCACCAT GCCGGCAGCA ATGGCGACCA
     2161 CCCTGCGTAA ACTGCTGACG GGTGAGCTGC TGACCCTGGC AAGCCGCCAG CAACTGATTG
     2221 ATTGGATGGA AGCGGATAAA GTGGCGGGTC CGCTGCTGCG TAGCGCGCTG CCGGCTGGCT
     2281 GGTTTATTGC GGATAAAAGC GGTGCGGGCG AACGTGGCAG CCGTGGCATT ATTGCGGCGC
     2341 TGGGCCCGGA TGGTAAACCG AGCCGTATTG TGGTGATTTA TACCACCGGC AGCCAGGCGA
     2401 CGATGGATGA ACGTAACCGT CAGATTGCGG AAATTGGCGC GAGCCTGATT AAACATTGGT
     2461 AAACCGATAC AATTAAAGGC TCCTTTTGGA GCCTTTTTTT TTGGACGACC CTTGTCCTTT
     2521 TCCGCTGCAT AACCCTGCTT CGGGGTCATT ATAGCGATTT TTTCGGTATA TCCATCCTTT
     2581 TTCGCACGAT ATACAGGATT TTGCCAAAGG GTTCGTGTAG ACTTTCCTTG GTGTATCCAA
     2641 CGGCGTCAGC CGGGCAGGAT AGGTGAAGTA GGCCCACCCG CGAGCGGGTG TTCCTTCTTC
     2701 ACTGTCCCTT ATTCGCACCT GGCGGTGCTC AACGGGAATC CTGCTCTGCG AGGCTGGCCG
     2761 TAGGCCGGCC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA
     2821 ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA
     2881 GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT
     2941 GTTCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA
     3001 TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT
     3061 ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG
     3121 GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG
     3181 CGTGAGCTTT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA
     3241 AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT
     3301 CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG
     3361 TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC
     3421 TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC
     3481 CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC
     3541 GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT
     3601 TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG
     3661 CGCAACGCAA TTAATGTGAG TTAGCTCACT CATTAGGCAG GCGCGCCCAG CTGTCTAGGG
     3721 CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA CAACAGATAA AACGAAAGGC
     3781 CCAGTCTTTC GACTGAGCCT TTCGTTTTAT TTGATGCCT  
//
