LOCUS       l06_Linker_67        3133 bp DNA     circular SYN 22-MAY-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   SBG
  TITLE     Direct Submission
  JOURNAL   Exported Jul 5, 2023 from SnapGene Viewer 6.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3133
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    125..146
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        161..191
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    199..215
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     223..239
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    298..301
                     /label=7
     misc_feature    302..330
                     /label=Linker_67
     misc_feature    331..334
                     /label=6
     primer_bind     complement(362..378)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      657..751
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        824..915
                     /gene="bla"
                     /label=AmpR promoter
     CDS             916..1776
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1780..1819
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            1966..2074
                     /note="incP origin of transfer"
     rep_origin      2110..2698
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2986..3007
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      3041..3127
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
BASE COUNT         716 a         813 c         843 g         761 t
ORIGIN
        1 ttaattaaga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
       61 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
      121 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
      181 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
      241 atgattacgc caagcttgca tgcctgcagg tcgactctag aggatccccg aagacaagct
      301 cccttaagga tagtttagcc taattatcta cacattgtct tcgggtaccg agctcgaatt
      361 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
      421 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
      481 gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc
      541 ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg
      601 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgaacta gtcttggact
      661 cctgttgata gatccagtaa tgacctcaga actccatctg gatttgttca gaacgctcgg
      721 ttgccgccgg gcgtttttta ttggtgagaa tccaggggtc cccaataatt acgatttaaa
      781 ttagtagccc gcctaatgag cgggcttttt tttaattccc ctatttgttt atttttctaa
      841 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
      901 tgaaaaagga agagtatgag cattcagcat tttcgtgtgg cgctgattcc gttttttgcg
      961 gcgttttgcc tgccggtgtt tgcgcatccg gaaaccctgg tgaaagtgaa agatgcggaa
     1021 gatcaactgg gtgcgcgcgt gggctatatt gaactggatc tgaacagcgg caaaattctg
     1081 gaatcttttc gtccggaaga acgttttccg atgatgagca cctttaaagt gctgctgtgc
     1141 ggtgcggttc tgagccgtgt ggatgcgggc caggaacaac tgggccgtcg tattcattat
     1201 agccagaacg atctggtgga atatagcccg gtgaccgaaa aacatctgac cgatggcatg
     1261 accgtgcgtg aactgtgcag cgcggcgatt accatgagcg ataacaccgc ggcgaacctg
     1321 ctgctgacga ccattggcgg tccgaaagaa ctgaccgcgt ttctgcataa catgggcgat
     1381 catgtgaccc gtctggatcg ttgggaaccg gaactgaacg aagcgattcc gaacgatgaa
     1441 cgtgatacca ccatgccggc agcaatggcg accaccctgc gtaaactgct gacgggtgag
     1501 ctgctgaccc tggcaagccg ccagcaactg attgattgga tggaagcgga taaagtggcg
     1561 ggtccgctgc tgcgtagcgc gctgccggct ggctggttta ttgcggataa aagcggtgcg
     1621 ggcgaacgtg gcagccgtgg cattattgcg gcgctgggcc cggatggtaa accgagccgt
     1681 attgtggtga tttataccac cggcagccag gcgacgatgg atgaacgtaa ccgtcagatt
     1741 gcggaaattg gcgcgagcct gattaaacat tggtaaaccg atacaattaa aggctccttt
     1801 tggagccttt ttttttggac gacccttgtc cttttccgct gcataaccct gcttcggggt
     1861 cattatagcg attttttcgg tatatccatc ctttttcgca cgatatacag gattttgcca
     1921 aagggttcgt gtagactttc cttggtgtat ccaacggcgt cagccgggca ggataggtga
     1981 agtaggccca cccgcgagcg ggtgttcctt cttcactgtc ccttattcgc acctggcggt
     2041 gctcaacggg aatcctgctc tgcgaggctg gccgtaggcc ggccccgtag aaaagatcaa
     2101 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     2161 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     2221 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     2281 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     2341 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     2401 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     2461 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctttgagaaa gcgccacgct
     2521 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     2581 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     2641 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     2701 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     2761 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     2821 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     2881 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     2941 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     3001 cactcattag gcaggcgcgc ccagctgtct agggcggcgg atttgtccta ctcaggagag
     3061 cgttcaccga caaacaacag ataaaacgaa aggcccagtc tttcgactga gcctttcgtt
     3121 ttatttgatg cct
//
