LOCUS       l08_Linker_BC        3107 bp DNA     circular SYN 11-JUL-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   SBG
  TITLE     Direct Submission
  JOURNAL   Exported Sep 26, 2023 from SnapGene Viewer 6.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3107
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      7..93
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    127..148
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(436..1024)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     oriT            1060..1168
                     /note="incP origin of transfer"
     terminator      1315..1354
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     CDS             complement(1358..2218)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2219..2310)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      2383..2477
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     2756..2772
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2799..2802
                     /label=B
     misc_feature    2803..2807
                     /label=Linker_BC
     misc_feature    2808..2811
                     /label=C
     primer_bind     complement(2869..2885)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2893..2909
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2917..2947)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2962..2983
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
       61 ttgtcggtga acgctctcct gagtaggaca aatccgccgc cctagacagc tgggcgcgcc
      121 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
      181 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
      241 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
      301 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
      361 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      421 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
      481 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      541 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      601 tctcccttcg ggaagcgtgg cgctttctca aagctcacgc tgtaggtatc tcagttcggt
      661 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
      721 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
      781 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
      841 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
      901 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
      961 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     1021 tcaagaagat cctttgatct tttctacggg gccggcctac ggccagcctc gcagagcagg
     1081 attcccgttg agcaccgcca ggtgcgaata agggacagtg aagaaggaac acccgctcgc
     1141 gggtgggcct acttcaccta tcctgcccgg ctgacgccgt tggatacacc aaggaaagtc
     1201 tacacgaacc ctttggcaaa atcctgtata tcgtgcgaaa aaggatggat ataccgaaaa
     1261 aatcgctata atgaccccga agcagggtta tgcagcggaa aaggacaagg gtcgtccaaa
     1321 aaaaaaggct ccaaaaggag cctttaattg tatcggttta ccaatgttta atcaggctcg
     1381 cgccaatttc cgcaatctga cggttacgtt catccatcgt cgcctggctg ccggtggtat
     1441 aaatcaccac aatacggctc ggtttaccat ccgggcccag cgccgcaata atgccacggc
     1501 tgccacgttc gcccgcaccg cttttatccg caataaacca gccagccggc agcgcgctac
     1561 gcagcagcgg acccgccact ttatccgctt ccatccaatc aatcagttgc tggcggcttg
     1621 ccagggtcag cagctcaccc gtcagcagtt tacgcagggt ggtcgccatt gctgccggca
     1681 tggtggtatc acgttcatcg ttcggaatcg cttcgttcag ttccggttcc caacgatcca
     1741 gacgggtcac atgatcgccc atgttatgca gaaacgcggt cagttctttc ggaccgccaa
     1801 tggtcgtcag cagcaggttc gccgcggtgt tatcgctcat ggtaatcgcc gcgctgcaca
     1861 gttcacgcac ggtcatgcca tcggtcagat gtttttcggt caccgggcta tattccacca
     1921 gatcgttctg gctataatga atacgacggc ccagttgttc ctggcccgca tccacacggc
     1981 tcagaaccgc accgcacagc agcactttaa aggtgctcat catcggaaaa cgttcttccg
     2041 gacgaaaaga ttccagaatt ttgccgctgt tcagatccag ttcaatatag cccacgcgcg
     2101 cacccagttg atcttccgca tctttcactt tcaccagggt ttccggatgc gcaaacaccg
     2161 gcaggcaaaa cgccgcaaaa aacggaatca gcgccacacg aaaatgctga atgctcatac
     2221 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     2281 tatttgaatg tatttagaaa aataaacaaa taggggaatt aaaaaaaagc ccgctcatta
     2341 ggcgggctac taatttaaat cgtaattatt ggggacccct ggattctcac caataaaaaa
     2401 cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg
     2461 atctatcaac aggagtccaa gactagttca gcgggtgttg gcgggtgtcg gggctggctt
     2521 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
     2581 cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac
     2641 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
     2701 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
     2761 acgacggcca gtgaattcga gctcggtacc cggtctcata ctcctaccca tagagaccgg
     2821 ggatcctcta gagtcgacct gcaggcatgc aagcttggcg taatcatggt catagctgtt
     2881 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     2941 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     3001 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     3061 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttct taattaa
//
