LOCUS       l14_Linker_HI        3113 bp DNA     circular SYN 29-JUL-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   SBG
  TITLE     Direct Submission
  JOURNAL   Exported Sep 26, 2023 from SnapGene Viewer 6.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3113
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    125..146
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        161..191
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    199..215
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     223..239
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    297..300
                     /label=H
     misc_feature    301..311
                     /label=Linker
     misc_feature    312..315
                     /label=I
     primer_bind     complement(342..358)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      637..731
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        804..895
                     /gene="bla"
                     /label=AmpR promoter
     CDS             896..1756
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1760..1799
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            1946..2054
                     /note="incP origin of transfer"
     rep_origin      2090..2678
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2966..2987
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      3021..3107
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaaga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
       61 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
      121 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
      181 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
      241 atgattacgc caagcttgca tgcctgcagg tcgactctag aggatccccg gtctcaggta
      301 tgactgtcgt acgctagaga ccgggtaccg agctcgaatt cactggccgt cgttttacaa
      361 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
      421 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
      481 agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
      541 tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
      601 ccccgacacc cgccaacacc cgctgaacta gtcttggact cctgttgata gatccagtaa
      661 tgacctcaga actccatctg gatttgttca gaacgctcgg ttgccgccgg gcgtttttta
      721 ttggtgagaa tccaggggtc cccaataatt acgatttaaa ttagtagccc gcctaatgag
      781 cgggcttttt tttaattccc ctatttgttt atttttctaa atacattcaa atatgtatcc
      841 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
      901 cattcagcat tttcgtgtgg cgctgattcc gttttttgcg gcgttttgcc tgccggtgtt
      961 tgcgcatccg gaaaccctgg tgaaagtgaa agatgcggaa gatcaactgg gtgcgcgcgt
     1021 gggctatatt gaactggatc tgaacagcgg caaaattctg gaatcttttc gtccggaaga
     1081 acgttttccg atgatgagca cctttaaagt gctgctgtgc ggtgcggttc tgagccgtgt
     1141 ggatgcgggc caggaacaac tgggccgtcg tattcattat agccagaacg atctggtgga
     1201 atatagcccg gtgaccgaaa aacatctgac cgatggcatg accgtgcgtg aactgtgcag
     1261 cgcggcgatt accatgagcg ataacaccgc ggcgaacctg ctgctgacga ccattggcgg
     1321 tccgaaagaa ctgaccgcgt ttctgcataa catgggcgat catgtgaccc gtctggatcg
     1381 ttgggaaccg gaactgaacg aagcgattcc gaacgatgaa cgtgatacca ccatgccggc
     1441 agcaatggcg accaccctgc gtaaactgct gacgggtgag ctgctgaccc tggcaagccg
     1501 ccagcaactg attgattgga tggaagcgga taaagtggcg ggtccgctgc tgcgtagcgc
     1561 gctgccggct ggctggttta ttgcggataa aagcggtgcg ggcgaacgtg gcagccgtgg
     1621 cattattgcg gcgctgggcc cggatggtaa accgagccgt attgtggtga tttataccac
     1681 cggcagccag gcgacgatgg atgaacgtaa ccgtcagatt gcggaaattg gcgcgagcct
     1741 gattaaacat tggtaaaccg atacaattaa aggctccttt tggagccttt ttttttggac
     1801 gacccttgtc cttttccgct gcataaccct gcttcggggt cattatagcg attttttcgg
     1861 tatatccatc ctttttcgca cgatatacag gattttgcca aagggttcgt gtagactttc
     1921 cttggtgtat ccaacggcgt cagccgggca ggataggtga agtaggccca cccgcgagcg
     1981 ggtgttcctt cttcactgtc ccttattcgc acctggcggt gctcaacggg aatcctgctc
     2041 tgcgaggctg gccgtaggcc ggccccgtag aaaagatcaa aggatcttct tgagatcctt
     2101 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     2161 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     2221 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     2281 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     2341 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     2401 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     2461 tgagatacct acagcgtgag ctttgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     2521 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     2581 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     2641 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     2701 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     2761 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     2821 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     2881 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     2941 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaggcgcgc
     3001 ccagctgtct agggcggcgg atttgtccta ctcaggagag cgttcaccga caaacaacag
     3061 ataaaacgaa aggcccagtc tttcgactga gcctttcgtt ttatttgatg cct
//
