LOCUS       Untitled_Sequence        3117 bp    DNA     circular SYN 28-FEB-2023
FEATURES             Location/Qualifiers
     primer_bind     346..362
                     /label="M13 fwd"
                     /color="#a020f0"
     primer_bind     223..239
                     /label="M13 rev"
                     /color="#a020f0"
     protein_bind    199..215
                     /label="lac operator"
                     /color="#31849b"
     protein_bind    2970..2991
                     /label="CAP binding site"
                     /color="#31849b"
     protein_bind    125..146
                     /label="CAP binding site"
                     /color="#31849b"
     promoter        185..191
                     /label="lac promoter"
                     /color="#ffffff"
     terminator      1764..1803
                     /label="fd terminator"
                     /color="#ffffff"
     terminator      3025..3111
                     /label="rrnB T1 terminator"
                     /color="#ffffff"
     promoter        808..899
                     /label="AmpR promoter"
                     /color="#ffffff"
     terminator      641..735
                     /label="lambda t0 terminator"
                     /color="#ffffff"
     oriT            1950..2058
                     /label="oriT"
                     /color="#a6acb3"
     rep_origin      2094..2682
                     /label="ori"
                     /color="#ffff00"
     CDS             969..1760
                     /label="AmpR"
                     /color="#ccffcc"
     misc_feature    297..300
                     /label="G"
                     /color="#00ffff"
     misc_feature    316..319
                     /label="I"
                     /color="#00ffff"
     misc_feature    301..315
                     /label="Linker 1_GI"
                     /color="#ff0000"
ORIGIN      
        1 TTAATTAAGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA
       61 TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG
      121 CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG
      181 CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC AGCTATGACC
      241 ATGATTACGC CAAGCTTGCA TGCCTGCAGG TCGACTCTAG AGGATCCCCG GTCTCAGCTT
      301 TCCCAGACCC ACCTTCGCTA GAGACCGGGT ACCGAGCTCG AATTCACTGG CCGTCGTTTT
      361 ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT AATCGCCTTG CAGCACATCC
      421 CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC GATCGCCCTT CCCAACAGTT
      481 GCGCAGCCTG AATGGCGAAT GGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG
      541 TATTTCACAC CGCATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG
      601 CCAGCCCCGA CACCCGCCAA CACCCGCTGA ACTAGTCTTG GACTCCTGTT GATAGATCCA
      661 GTAATGACCT CAGAACTCCA TCTGGATTTG TTCAGAACGC TCGGTTGCCG CCGGGCGTTT
      721 TTTATTGGTG AGAATCCAGG GGTCCCCAAT AATTACGATT TAAATTAGTA GCCCGCCTAA
      781 TGAGCGGGCT TTTTTTTAAT TCCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT
      841 ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA
      901 TGAGCATTCA GCATTTTCGT GTGGCGCTGA TTCCGTTTTT TGCGGCGTTT TGCCTGCCGG
      961 TGTTTGCGCA TCCGGAAACC CTGGTGAAAG TGAAAGATGC GGAAGATCAA CTGGGTGCGC
     1021 GCGTGGGCTA TATTGAACTG GATCTGAACA GCGGCAAAAT TCTGGAATCT TTTCGTCCGG
     1081 AAGAACGTTT TCCGATGATG AGCACCTTTA AAGTGCTGCT GTGCGGTGCG GTTCTGAGCC
     1141 GTGTGGATGC GGGCCAGGAA CAACTGGGCC GTCGTATTCA TTATAGCCAG AACGATCTGG
     1201 TGGAATATAG CCCGGTGACC GAAAAACATC TGACCGATGG CATGACCGTG CGTGAACTGT
     1261 GCAGCGCGGC GATTACCATG AGCGATAACA CCGCGGCGAA CCTGCTGCTG ACGACCATTG
     1321 GCGGTCCGAA AGAACTGACC GCGTTTCTGC ATAACATGGG CGATCATGTG ACCCGTCTGG
     1381 ATCGTTGGGA ACCGGAACTG AACGAAGCGA TTCCGAACGA TGAACGTGAT ACCACCATGC
     1441 CGGCAGCAAT GGCGACCACC CTGCGTAAAC TGCTGACGGG TGAGCTGCTG ACCCTGGCAA
     1501 GCCGCCAGCA ACTGATTGAT TGGATGGAAG CGGATAAAGT GGCGGGTCCG CTGCTGCGTA
     1561 GCGCGCTGCC GGCTGGCTGG TTTATTGCGG ATAAAAGCGG TGCGGGCGAA CGTGGCAGCC
     1621 GTGGCATTAT TGCGGCGCTG GGCCCGGATG GTAAACCGAG CCGTATTGTG GTGATTTATA
     1681 CCACCGGCAG CCAGGCGACG ATGGATGAAC GTAACCGTCA GATTGCGGAA ATTGGCGCGA
     1741 GCCTGATTAA ACATTGGTAA ACCGATACAA TTAAAGGCTC CTTTTGGAGC CTTTTTTTTT
     1801 GGACGACCCT TGTCCTTTTC CGCTGCATAA CCCTGCTTCG GGGTCATTAT AGCGATTTTT
     1861 TCGGTATATC CATCCTTTTT CGCACGATAT ACAGGATTTT GCCAAAGGGT TCGTGTAGAC
     1921 TTTCCTTGGT GTATCCAACG GCGTCAGCCG GGCAGGATAG GTGAAGTAGG CCCACCCGCG
     1981 AGCGGGTGTT CCTTCTTCAC TGTCCCTTAT TCGCACCTGG CGGTGCTCAA CGGGAATCCT
     2041 GCTCTGCGAG GCTGGCCGTA GGCCGGCCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT
     2101 CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG
     2161 GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA
     2221 GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC
     2281 TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT
     2341 GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG
     2401 CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC
     2461 GAACTGAGAT ACCTACAGCG TGAGCTTTGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG
     2521 GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA
     2581 GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT
     2641 CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC
     2701 TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC
     2761 CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC
     2821 CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC AATACGCAAA
     2881 CCGCCTCTCC CCGCGCGTTG GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC
     2941 TGGAAAGCGG GCAGTGAGCG CAACGCAATT AATGTGAGTT AGCTCACTCA TTAGGCAGGC
     3001 GCGCCCAGCT GTCTAGGGCG GCGGATTTGT CCTACTCAGG AGAGCGTTCA CCGACAAACA
     3061 ACAGATAAAA CGAAAGGCCC AGTCTTTCGA CTGAGCCTTT CGTTTTATTT GATGCCT
//