LOCUS       lv1_119                 5105 bp ds-DNA     circular     12-JUL-2022
DEFINITION  .
KEYWORDS    "creator:Centro Nacional De Biotecnologia" "marker:KanR, rhaR"     
FEATURES             Location/Qualifiers
     terminator      7..93
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     Ori             complement(121..1642)
                     /label="pBBR1* Ori"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     rep_origin      208..979
                     /label="pBBR1 oriV"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             980..1642
                     /label="pBBR1 Rep"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     oriT            1653..1761
                     /label="oriT"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             complement(1928..2743)
                     /label="KanR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     terminator      2875..2969
                     /label="lambda t0 terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     Fusion site     3060..3063
                     /label="1"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     Promoter        3064..3194
                     /label="PrhaSR"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     RBS             3191..3216
                     /label="r7 (RBS_ST)"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     RBS             3200..3208
                     /label="Shine-Dalgarno sequence"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             3214..4050
                     /label="rhaS"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     CDS             4124..4972
                     /label="rhaR"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     Terminator      4973..5019
                     /label="te6 (BBa_B1006)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     Fusion site     5020..5023
                     /label="2"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
ORIGIN
        1 AGGCATCAAA TAAAACGAAA GGCTCAGTCG AAAGACTGGG CCTTTCGTTT TATCTGTTGT
       61 TTGTCGGTGA ACGCTCTCCT GAGTAGGACA AATCCGCCGC CCTAGACAGC TGGGCGCGCC
      121 CCCCCTACGG GCTTGCTCTC CGGGCTTCGC CCTGCGCGGT CGCTGCGCTC CCTTGCCAGC
      181 CCGTGGATAT GTGGACGATG GCCGCGAGCG GCCACCGGCT GGCTCGCTTC GCTCGGCCCG
      241 TGGACAACCC TGCTGGACAA GCTGATGGAC AGGCTGCGCC TGCCCACGAG CTTGACCACA
      301 GGGATTGCCC ACCGGCTACC CAGCCTTCGA CCACATACCC ACCGGCTCCA ACTGCGCGGC
      361 CTGCGGCCTT GCCCCATCAA TTTTTTTAAT TTTCTCTGGG GAAAAGCCTC CGGCCTGCGG
      421 CCTGCGCGCT TCGCTTGCCG GTTGGACACC AAGTGGAAGG CGGGTCAAGG CTCGCGCAGC
      481 GACCGCGCAG CGGCTTGGCC TTGACGCGCC TGGAACGACC CAAGCCTATG CGAGTGGGGG
      541 CAGTCGAAGG GCGAAGCCCG CCCGCCTGCC CCCCGAGCCT CACGGCGGCG AGTGCGGGGG
      601 TTCCAAGGGG GCAGCGCCAC CTTGGGCAAG GCCGAAGGCC GCGCAGTCGA TCAACAAGCC
      661 CCGGAGGGGC CACTTTTTGC CGGAGGGGGA GCCGCGCCGA AGGCGTGGGG GAACCCCGCA
      721 GGGGTGCCCT TCTTTGGGCA CCAAAGAACT AGATATAGGG CGAAATGCGA AAGACTTAAA
      781 AATCAACAAC TTAAAAAAGG GGGGTACGCA ACAGCTCATT GCGGCACCCC CCGCAATAGC
      841 TCATTGCGTA GGTTAAAGAA AATCTGTAAT TGACTGCCAC TTTTACGCAA CGCATAATTG
      901 TTGTCGCGCT GCCGAAAAGT TGCAGCTGAT TGCGCATGGT GCCGCAACCG TGCGGCACCC
      961 CTACCGCATG GAGATAAGCA TGGCCACGCA GTCCAGAGAA ATCGGCATTC AAGCCAAGAA
     1021 CAAGCCCGGT CACTGGGTGC AAACGGAACG CAAAGCGCAT GAGGCGTGGG CCGGGCTTAT
     1081 TGCGAGGAAA CCCACGGCGG CAATGCTGCT GCATCACCTC GTGGCGCAGA TGGGCCACCA
     1141 GAACGCCGTG GTGGTCAGCC AGAAAACACT TTCCAAGCTC ATCGGACGTT CTTTGCGGAC
     1201 GGTCCAATAC GCAGTCAAGG ACTTGGTGGC CGAGCGCTGG ATCTCCGTCG TGAAGCTCAA
     1261 CGGCCCCGGC ACCGTGTCGG CCTACGTGGT CAATGACCGC GTGGCGTGGG GCCAGCCCCG
     1321 CGACCAGTTG CGCCTGTCGG TGTTCAGTGC CGCCGTGGTG GTTGATCACG ACGACCAGGA
     1381 CGAATCGCTG TTGGGGCATG GCGACCTGCG CCGCATCCCG ACCCTGTATC CGGGCGAGCA
     1441 GCAACTACCG ACCGGCCCCG GCGAGGAGCC GCCCAGCCAG CCCGGCATTC CGGGCATGGA
     1501 ACCAGACCTG CCAGCCTTGA CCGAAACGGA GGAATGGGAA CGGCGCGGGC AGCAGCGCCT
     1561 GCCGATGCCC GATGAGCCGT GTTTTCTGGA CGATGGCGAG CCGTTGGAGC CGCCGACACG
     1621 GGTAACGCTG CCGCGCCGGT AGGGCCGGCC TACGGCCAGC CTCGCAGAGC AGGATTCCCG
     1681 TTGAGCACCG CCAGGTGCGA ATAAGGGACA GTGAAGAAGG AACACCCGCT CGCGGGTGGG
     1741 CCTACTTCAC CTATCCTGCC CGGCTGACGC CGTTGGATAC ACCAAGGAAA GTCTACACGA
     1801 ACCCTTTGGC AAAATCCTGT ATATCGTGCG AAAAAGGATG GATATACCGA AAAAATCGCT
     1861 ATAATGACCC CGAAGCAGGG TTATGCAGCG GAAAAGGACA ACGCGCGGAC CGCGGTCCAA
     1921 TTAATTATTA GAAAAATTCA TCCAGCATCA GATGAAATTG CAGTTTGTTC ATATCCGGAT
     1981 TATCAATGCC ATATTTCTGA AACAGACGTT TTTGCAGGCT CGGGCTAAAT TCGCCCAGGC
     2041 AGTTCCACAG AATGGCCAGA TCCTGATAAC GATCCGCAAT GCCCACACGG CCCACATCAA
     2101 TGCAGCCAAT CAGTTTGCCT TCATCGAAAA TCAGGTTATC CAGGCTAAAA TCGCCGTGGG
     2161 TCACCACGCT ATCCGGGCTA AACGGCAGCA GTTTATGCAT TTCTTTCCAC ACCTGTTCCA
     2221 CCGGCCAGCC GTTACGTTCA TCATCAAAAT CGCTCGCATC CACCAGGCCG TTGTTCATAC
     2281 GGCTCTGCGC CTGGGCCAGA CGAAACACAC GATCGCTGTT AAACGGGCAG TTGCACACCG
     2341 GAATGCTATG CAGACGACGC AGAAACACGG CCAGCGCATC CACAATGTTT TCGCCGCTAT
     2401 CCGGATATTC TTCCAGCACC TGAAACGCGG TTTTGCCCGG AATCGCGGTG GTCAGCAGCC
     2461 ACGCATCATC CGGGGTGCGA ATAAAATGTT TAATGGTCGG CAGCGGCATA AATTCGGTCA
     2521 GCCAGTTCAG ACGCACCATT TCATCGGTCA CATCGTTCGC CACGCTGCCT TTGCCATGTT
     2581 TCAGAAACAG TTCCGGCGCA TCCGGTTTGC CATACAGACG ATAAATGGTC GCGCCGCTCT
     2641 GACCCACGTT ATCACGCGCC CATTTATAGC CATACAGATC CGCATCCATG TTGCTGTTCA
     2701 GACGCGGACG GCTACAGCTC GTTTCACGCT GAATATGGCT CATAACACCC CTTGTATTAC
     2761 TGTTTATGTA AGCAGACAGT TTTATTGTTC ATGATGATAT ATTTTTATCT TGTGCAATGT
     2821 AACATCAGAG ATTTTGAGAC ACAAATTTAA ATCGTAATTA TTGGGGACCC CTGGATTCTC
     2881 ACCAATAAAA AACGCCCGGC GGCAACCGAG CGTTCTGAAC AAATCCAGAT GGAGTTCTGA
     2941 GGTCATTACT GGATCTATCA ACAGGAGTCC AAGACTAGTC GCCAGGGTTT TCCCAGTCAC
     3001 GACGCGGCCG CAAGCTTGCA TGCCTGCAGG TCGACTCTAG AGGATCCCCG GGAAGACAAT
     3061 GCCGGAGTGA TGTGATGCTC ACCGCATTTC CTGAAAATTC ACGCTGTATC TTGAAAAATC
     3121 GACGTTTTTT ACGTGGTTTT CCGTCGAAAA TTTAAGGTAA GAACCTGACC TCGTGATTAC
     3181 TATTTCGCCG TACTTGACCT AAGGAGGTAA ATAATGACCG TATTACATAG TGTGGATTTT
     3241 TTTCCGTCTG GTAACGCGTC CGTGGCGATA GAACCCCGGC TCCCGCAGGC GGATTTTCCT
     3301 GAACATCATC ATGATTTTCA TGAAATTGTG ATTGTCGAAC ATGGCACGGG TATTCATGTG
     3361 TTTAATGGGC AGCCCTATAC CATCACCGGT GGCACGGTCT GTTTCGTACG CGATCATGAT
     3421 CGGCATCTGT ATGAACATAC CGATAATCTG TGTCTGACCA ATGTGCTGTA TCGCTCGCCG
     3481 GATCGATTTC AGTTTCTCGC CGGGCTGAAT CAGTTGCTGC CACAAGAGCT GGATGGGCAG
     3541 TATCCGTCTC ACTGGCGCGT TAACCACAGC GTATTGCAGC AGGTGCGACA GCTGGTTGCA
     3601 CAGATGGAAC AGCAGGAAGG GGAAAATGAT TTACCCTCGA CCGCCAGTCG CGAGATCTTG
     3661 TTTATGCAAT TACTGCTCTT GCTGCGTAAA AGCAGTTTGC AGGAGAACCT GGAAAACAGC
     3721 GCATCACGTC TCAACTTGCT TCTGGCCTGG CTGGAGGACC ATTTTGCCGA TGAGGTGAAT
     3781 TGGGATGCCG TGGCGGATCA ATTTTCTCTT TCACTGCGTA CGCTACATCG GCAGCTTAAG
     3841 CAGCAAACGG GACTGACGCC TCAGCGATAC CTGAACCGCC TGCGACTGAT GAAAGCCCGA
     3901 CATCTGCTAC GCCACAGCGA GGCCAGCGTT ACTGACATCG CCTATCGCTG TGGATTCAGC
     3961 GACAGTAACC ACTTTTCGAC GCTTTTTCGC CGAGAGTTTA ACTGGTCACC GCGTGATATT
     4021 CGCCAGGGAC GGGATGGCTT TCTGCAATAA CGCGAATCTT CTCAACGTAT TTGTACGCCA
     4081 TATTGCGAAT AATCAACTTC GTTCTCTGGC CGAGGTAGCC ACGGTGGCGC ATCAGTTAAA
     4141 ACTTCTCAAA GATGATTTTT TTGCCAGCGA CCAGCAGGCA GTCGCTGTGG CTGACCGTTA
     4201 TCCGCAAGAT GTCTTTGCTG AACATACACA TGATTTTTGT GAGCTGGTGA TTGTCTGGCG
     4261 CGGTAATGGC CTGCATGTAC TCAACGATCG CCCTTATCGC ATTACCCGTG GCGATCTCTT
     4321 TTACATTCAT GCTGACGATA AACACTCCTA CGCTTCCGTT AACGATCTGG TTTTGCAGAA
     4381 TATTATTTAT TGCCCGGAGC GTCTGAAGCT GAATCTTGAC TGGCAGGGGG CGATTCCGGG
     4441 ATTTAACGCC AGCGCAGGGC AACCACACTG GCGCTTAGGT AGCATGGGGA TGGCGCAGGC
     4501 GCGGCAGGTT ATCGGTCAGC TTGAGCATGA AAGTAGTCAG CATGTGCCGT TTGCTAACGA
     4561 AATGGCTGAG TTGCTGTTCG GGCAGTTGGT GATGTTGCTG AATCGCCATC GTTACACCAG
     4621 TGATTCGTTG CCGCCAACAT CCAGCGAAAC GTTGCTGGAT AAGCTGATTA CCCGGCTGGC
     4681 GGCTAGCCTG AAAAGTCCCT TTGCGCTGGA TAAATTTTGT GATGAGGCAT CGTGCAGTGA
     4741 GCGCGTTTTG CGTCAGCAAT TTCGCCAGCA GACTGGAATG ACCATCAATC AATATCTGCG
     4801 ACAGGTCAGA GTGTGTCATG CGCAATATCT TCTCCAGCAT AGCCGCCTGT TAATCAGTGA
     4861 TATTTCGACC GAATGTGGCT TTGAAGATAG TAACTATTTT TCGGTGGTGT TTACCCGTGA
     4921 AACCGGGATG ACGCCCAGCC AGTGGCGTCA TCTCAATTCG CAGAAAGATT GAGCTTAAAA
     4981 AAAAACCCCG CCCCTGACAG GGCGGGGTTT TTTTTCGCTG CAATTGTCTT CGGGTACCGA
     5041 GCTCGAATTC GCGCGGCCGC GGCCTAGGCG GCCTCCTGTG TGAAATTGTT ATCCGCTTTA
     5101 ATTAA
//