LOCUS       Untitled_Sequence        4462 bp    DNA     circular SYN 28-FEB-2023
FEATURES             Location/Qualifiers
     CDS             2313..3128
                     /label="KanR"
                     /color="#ccffcc"
     rep_origin      3439..4027
                     /label="ori"
                     /color="#ffff00"
     oriT            3295..3404
                     /label="oriT"
                     /color="#a6acb3"
     terminator      2087..2181
                     /label="lambda t0 terminator"
                     /color="#ffffff"
     terminator      4370..4456
                     /label="rrnB T1 terminator"
                     /color="#ffffff"
     protein_bind    4315..4336
                     /label="CAP binding site"
                     /color="#31849b"
     CDS             154..1119
                     /label="XylS"
                     /color="#993366"
     misc_feature    83..86
                     /label="1"
                     /color="#99cc00"
     terminator      87..143
                     /label="ECK120033737 terminator"
                     /color="#ffffff"
     misc_feature    144..1381
                     /label="pWW0"
                     /color="#a6acb3"
     promoter        1261..1312
                     /label="xylS promoter"
                     /color="#ffffff"
     misc_feature    1382..1993
                     /label="Buffer sequence"
                     /color="#c0c0c0"
     misc_feature    1994..1997
                     /label="2"
                     /color="#99cc00"
ORIGIN      
        1 TTAATTAAAG CGGATAACAA TTTCACACAG GAGGCCGCCT AGGCCGCGGC CGCGCGAATT
       61 CGAGCTCGGT ACCCGAAGAC AATGCCCCTT TGGTCGAAAA AAAAAGCCCG CACTGTCAGG
      121 TGCGGGCTTT TTTCTGTGTT TCCACGTTCG TAATCAAGCC ACTTCCTTTT TGCATTGACG
      181 CAGGGTGTCG GAAGGCAACT CGCCGAACGC GCTCCTATAG TTTTCAGCGA AGCGTCCCAA
      241 ATGTAAGAAG CCGTAGTCTA GGGCTATCTC AGTTATACTA CGCACATTGG CACTGGGATC
      301 GTTCAAGCAG GCGCGGATGC TTTCGAGCTT GCGGTTGCGG ATGTAGTTCT TCGGCGTGGT
      361 GCCGGCGTGC TTCTCGAACA AATTGTAGAG CGAGCGTGGA CTCATCATCG CCAGCTCCGC
      421 TAACCGCTCA AGGCTGATAT TCCGTTTGAG ATTCTCCTCA ATGAATTGAA CGACTCGCTC
      481 GAAAGACGGG TTACCTTTGC TGAAAATTTC ACGGCTGACA TTGCTGCCCA GCATTTCGAG
      541 CAGCTTGGAA GCGATGATCC CCGCATAGTG CTCTTGGACC CGAGGCATCG ACTTTGTATG
      601 TTCCGCTTCG TCACAAACTA ACCCGAGTAG ATTGATAAAG CCATCGAGTT GCTGGAGATT
      661 GTGTCGCGCG GCGAAACGGA TACCCTCCCT CGGCTTGTGC CAATTGTTGT CACTGCACGC
      721 CCGATCAAGG ACCACTGAGG GCAATTTAAC GATAAATTTC TCGCAATCTT CTGAATAGGT
      781 CAGGTCGGCT TGGTCATCCG GATTGAGCAG CAATAGTTCG CCCGGCGCAA AATAGTGCTC
      841 CTGGCCATGG CCACGCCACA GGCAATGGCC TTTGAGTATT ATTTGCAGAT GATAACAGGT
      901 TTCTAATCCA GGCGAGATTA CCCTCACGCT ACCGCCGTAG CTGATTCGAC ACAGATCGAG
      961 GCATCCGAAG ATTCTGTGGT GCAGCCTGCC TGCCGGGCGC CCGCCCTTGG GCAGGCGAAT
     1021 AGAGTGCGTA CCGACATACT GGTTAACATA ATCGGAGACT GCATAGGGCT CGGCGTGGAC
     1081 GAAGATCTGA CTTTTCTCGT TCAATAAGCA AAAATCCATA GTTCACGGTT CTCTTATTTT
     1141 AATGTGGGCT GCTTGGTGTG ATGTAGAAAG GCGCCAAGTC GATGAAAATG CATCTCGACG
     1201 TGATGCGTAT ACGGGTTACC CCCATTGCCA CGTTGCGCCA TCCTTTTTGC AATCAGTGAC
     1261 CACTTTTCCA AGCAAAAATA ACGCCAAGCA GAACGAACAC GTTCTTTTTA AGAAGCGAGA
     1321 ACACCAGAAG TTCGTGCTGT CGGGGCATGG GGCGACGAAT TGGCGGATAA AGGGGATCTG
     1381 CTGGATATTA CGGCCTTTTT AAAGACCGTA AAGAAAAATA AGCACAAGTT TTATCCGGCC
     1441 TTTATTCACA TTCTTGCCCG CCTGATGAAT GCTCATCCGT AATTACGTAT GGCAATGAAA
     1501 GACGGTGAGC TGGTGATATG GGATAGTGTT CACCCTTGTT ACACCGTTTT CCATGAGCAA
     1561 ACTGAAACGT TTTCATCGCT CTGGAGTGAA TACCACGACG ATTTCCGGCA GTTTCTACAC
     1621 ATATATTCGC AAGATGTGGC GTGTTACGGT GAAAACCTGG CCTATTTCCC TAAAGGGTTT
     1681 ATTGAGAATA TGTTTTTCGT CTCAGCCAAT CCCTGGGTGA GTTTCACCAG TTTTGATTTA
     1741 AACGTGGCCA ATATGGACAA CTTCTTCGCC CCCGTTTTCA CCATGCATGG GAATTAGCTT
     1801 GATCTGACCA ACGACCGGTA GCGGAGCTAT CCAACGGCGG TATACCAGGA AAACACACAG
     1861 CAGGTACATC AGAACAGTAC CATGACTGAA GAACAAATAG TTTTTTCCTG ATCCATAAAG
     1921 CAGAACGGCC TGCTCCATGA CAAATCTGGC TCCCCAACTA ATGCCCCATG CAGCCAGCAT
     1981 AACCAGCATA AAGGCAATTG TCTTCGGGGA TCCTCTAGAG TCGACCTGCA GGCATGCAAG
     2041 CTTGCGGCCG CGTCGTGACT GGGAAAAACC CTGGCGACTA GTCTTGGACT CCTGTTGATA
     2101 GATCCAGTAA TGACCTCAGA ACTCCATCTG GATTTGTTCA GAACGCTCGG TTGCCGCCGG
     2161 GCGTTTTTTA TTGGTGAGAA TCCAGGGGTC CCCAATAATT ACGATTTAAA TTTGTGTCTC
     2221 AAAATCTCTG ATGTTACATT GCACAAGATA AAAATATATC ATCATGAACA ATAAAACTGT
     2281 CTGCTTACAT AAACAGTAAT ACAAGGGGTG TTATGAGCCA TATTCAGCGT GAAACGAGCT
     2341 GTAGCCGTCC GCGTCTGAAC AGCAACATGG ATGCGGATCT GTATGGCTAT AAATGGGCGC
     2401 GTGATAACGT GGGTCAGAGC GGCGCGACCA TTTATCGTCT GTATGGCAAA CCGGATGCGC
     2461 CGGAACTGTT TCTGAAACAT GGCAAAGGCA GCGTGGCGAA CGATGTGACC GATGAAATGG
     2521 TGCGTCTGAA CTGGCTGACC GAATTTATGC CGCTGCCGAC CATTAAACAT TTTATTCGCA
     2581 CCCCGGATGA TGCGTGGCTG CTGACCACCG CGATTCCGGG CAAAACCGCG TTTCAGGTGC
     2641 TGGAAGAATA TCCGGATAGC GGCGAAAACA TTGTGGATGC GCTGGCCGTG TTTCTGCGTC
     2701 GTCTGCATAG CATTCCGGTG TGCAACTGCC CGTTTAACAG CGATCGTGTG TTTCGTCTGG
     2761 CCCAGGCGCA GAGCCGTATG AACAACGGCC TGGTGGATGC GAGCGATTTT GATGATGAAC
     2821 GTAACGGCTG GCCGGTGGAA CAGGTGTGGA AAGAAATGCA TAAACTGCTG CCGTTTAGCC
     2881 CGGATAGCGT GGTGACCCAC GGCGATTTTA GCCTGGATAA CCTGATTTTC GATGAAGGCA
     2941 AACTGATTGG CTGCATTGAT GTGGGCCGTG TGGGCATTGC GGATCGTTAT CAGGATCTGG
     3001 CCATTCTGTG GAACTGCCTG GGCGAATTTA GCCCGAGCCT GCAAAAACGT CTGTTTCAGA
     3061 AATATGGCAT TGATAATCCG GATATGAACA AACTGCAATT TCATCTGATG CTGGATGAAT
     3121 TTTTCTAATA ATTAATTGGA CCGCGGTCCG CGCGTTGTCC TTTTCCGCTG CATAACCCTG
     3181 CTTCGGGGTC ATTATAGCGA TTTTTTCGGT ATATCCATCC TTTTTCGCAC GATATACAGG
     3241 ATTTTGCCAA AGGGTTCGTG TAGACTTTCC TTGGTGTATC CAACGGCGTC AGCCGGGCAG
     3301 GATAGGTGAA GTAGGCCCAC CCGCGAGCGG GTGTTCCTTC TTCACTGTCC CTTATTCGCA
     3361 CCTGGCGGTG CTCAACGGGA ATCCTGCTCT GCGAGGCTGG CCGTAGGCCG GCCCCGTAGA
     3421 AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC
     3481 AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT
     3541 TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTTCTTC TAGTGTAGCC
     3601 GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT
     3661 CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG
     3721 ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC
     3781 CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TTTGAGAAAG
     3841 CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC
     3901 AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG
     3961 GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT
     4021 ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC
     4081 TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA
     4141 GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA
     4201 AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG
     4261 CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG CAATTAATGT
     4321 GAGTTAGCTC ACTCATTAGG CAGGCGCGCC CAGCTGTCTA GGGCGGCGGA TTTGTCCTAC
     4381 TCAGGAGAGC GTTCACCGAC AAACAACAGA TAAAACGAAA GGCCCAGTCT TTCGACTGAG
     4441 CCTTTCGTTT TATTTGATGC CT   
//
