LOCUS       Untitled_Sequence        4461 bp    DNA     circular SYN 28-FEB-2023
FEATURES             Location/Qualifiers
     terminator      1363..1390
                     /label="T7Te terminator"
                     /color="#ffffff"
     terminator      1276..1347
                     /label="rrnB T1 terminator"
                     /color="#ffffff"
     promoter        100..177
                     /label="lacIq promoter"
                     /color="#ffffff"
     terminator      4369..4455
                     /label="rrnB T1 terminator"
                     /color="#ffffff"
     terminator      1493..1587
                     /label="lambda t0 terminator"
                     /color="#ffffff"
     oriT            2701..2809
                     /label="oriT"
                     /color="#a6acb3"
     CDS             2820..3482
                     /label="pBBR1 Rep"
                     /color="#993366"
     rep_origin      3483..4254
                     /label="pBBR1 oriV"
                     /color="#ffff00"
     CDS             1719..2534
                     /label="KanR"
                     /color="#ccffcc"
     CDS             178..1260
                     /label="lacI"
                     /color="#993366"
     misc_feature    1401..1404
                     /label="2"
                     /color="#99cc00"
     misc_feature    85..88
                     /label="1"
                     /color="#99cc00"
ORIGIN      
        1 TTAATTAAAG CGGATAACAA TTTCACACAG GAGGCCGCCT AGGCCGCGGC CGCGCGAATT
       61 CGAGCTCGGT ACCCGGGAAG ACAATGCCTA AAGGTCGTTG ACACCATCGA ATGGTGCAAA
      121 ACCTTTCGCG GTATGGCATG ATAGCGCCCG GAAGAGAGTC AATTCAGGGT GGTGAATATG
      181 AAACCAGTGA CGTTGTACGA TGTCGCAGAG TATGCCGGTG TCTCGTATCA GACCGTTTCC
      241 CGCGTGGTGA ACCAGGCCAG CCACGTTTCT GCGAAAACGC GGGAAAAAGT GGAAGCGGCG
      301 ATGGCGGAGC TGAATTACAT TCCCAACCGC GTGGCACAAC AACTGGCGGG CAAACAGTCG
      361 TTGCTGATTG GCGTTGCCAC CTCCAGTCTG GCCCTGCACG CGCCGTCGCA AATTGTCGCG
      421 GCGATTAAAT CGCGCGCCGA TCAACTGGGT GCCAGCGTGG TGGTGTCGAT GGTGGAACGA
      481 AGCGGCGTCG AAGCCTGTAA AGCGGCGGTG CACAATCTTC TCGCGCAACG CGTCAGTGGG
      541 CTGATCATTA ACTATCCGCT GGATGACCAG GATGCCATTG CTGTGGAAGC TGCCTGCACT
      601 AATGTTCCGG CGTTGTTTCT TGATGTCTCG GACCAGACAC CCATCAACAG TATTATTTTC
      661 TCCCATGAGG ACGGTACGCG ACTGGGCGTG GAGCATCTGG TCGCATTGGG TCACCAGCAA
      721 ATCGCGCTGT TGGCGGGCCC ATTAAGTTCT GTCTCGGCGC GCCTGCGTCT GGCTGGCTGG
      781 CATAAATATC TCACTCGCAA TCAAATTCAG CCGATCGCGG AACGGGAAGG CGACTGGAGT
      841 GCCATGTCCG GTTTTCAACA AACCATGCAA ATGCTGAATG AGGGCATCGT TCCCACTGCG
      901 ATGCTGGTTG CCAACGATCA GATGGCGCTG GGCGCAATGC GCGCCATTAC CGAGTCCGGG
      961 CTGCGCGTTG GTGCGGATAT CTCGGTAGTG GGATACGACG ATACCGAAGA TAGCTCATGT
     1021 TATATCCCGC CGTTAACCAC CATCAAACAG GATTTTCGCC TGCTGGGGCA AACCAGCGTG
     1081 GACCGCTTGC TGCAACTCTC GCAGGGCCAG GCGGTGAAGG GCAATCAGCT GTTGCCCGTC
     1141 TCACTGGTGA AACGAAAAAC CACCCTGGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG
     1201 TTGGCCGATT CGTTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA
     1261 TAAAGGTCCA GGCATCAAAT AAAACGAAAG GCTCAGTCGA AAGACTGGGC CTTTCGTTTT
     1321 ATCTGTTGTT TGTCGGTGAA CGCTCTCTAC TAGAGTCACA CTGGCTCACC TTCGGGTGGG
     1381 CCTTTCTGCG TTTATAGTCG GCAATTGTCT TCGGGGATCC TCTAGAGTCG ACCTGCAGGC
     1441 ATGCAAGCTT GCGGCCGCGT CGTGACTGGG AAAACCCTGG CGACTAGTCT TGGACTCCTG
     1501 TTGATAGATC CAGTAATGAC CTCAGAACTC CATCTGGATT TGTTCAGAAC GCTCGGTTGC
     1561 CGCCGGGCGT TTTTTATTGG TGAGAATCCA GGGGTCCCCA ATAATTACGA TTTAAATTTG
     1621 TGTCTCAAAA TCTCTGATGT TACATTGCAC AAGATAAAAA TATATCATCA TGAACAATAA
     1681 AACTGTCTGC TTACATAAAC AGTAATACAA GGGGTGTTAT GAGCCATATT CAGCGTGAAA
     1741 CGAGCTGTAG CCGTCCGCGT CTGAACAGCA ACATGGATGC GGATCTGTAT GGCTATAAAT
     1801 GGGCGCGTGA TAACGTGGGT CAGAGCGGCG CGACCATTTA TCGTCTGTAT GGCAAACCGG
     1861 ATGCGCCGGA ACTGTTTCTG AAACATGGCA AAGGCAGCGT GGCGAACGAT GTGACCGATG
     1921 AAATGGTGCG TCTGAACTGG CTGACCGAAT TTATGCCGCT GCCGACCATT AAACATTTTA
     1981 TTCGCACCCC GGATGATGCG TGGCTGCTGA CCACCGCGAT TCCGGGCAAA ACCGCGTTTC
     2041 AGGTGCTGGA AGAATATCCG GATAGCGGCG AAAACATTGT GGATGCGCTG GCCGTGTTTC
     2101 TGCGTCGTCT GCATAGCATT CCGGTGTGCA ACTGCCCGTT TAACAGCGAT CGTGTGTTTC
     2161 GTCTGGCCCA GGCGCAGAGC CGTATGAACA ACGGCCTGGT GGATGCGAGC GATTTTGATG
     2221 ATGAACGTAA CGGCTGGCCG GTGGAACAGG TGTGGAAAGA AATGCATAAA CTGCTGCCGT
     2281 TTAGCCCGGA TAGCGTGGTG ACCCACGGCG ATTTTAGCCT GGATAACCTG ATTTTCGATG
     2341 AAGGCAAACT GATTGGCTGC ATTGATGTGG GCCGTGTGGG CATTGCGGAT CGTTATCAGG
     2401 ATCTGGCCAT TCTGTGGAAC TGCCTGGGCG AATTTAGCCC GAGCCTGCAA AAACGTCTGT
     2461 TTCAGAAATA TGGCATTGAT AATCCGGATA TGAACAAACT GCAATTTCAT CTGATGCTGG
     2521 ATGAATTTTT CTAATAATTA ATTGGACCGC GGTCCGCGCG TTGTCCTTTT CCGCTGCATA
     2581 ACCCTGCTTC GGGGTCATTA TAGCGATTTT TTCGGTATAT CCATCCTTTT TCGCACGATA
     2641 TACAGGATTT TGCCAAAGGG TTCGTGTAGA CTTTCCTTGG TGTATCCAAC GGCGTCAGCC
     2701 GGGCAGGATA GGTGAAGTAG GCCCACCCGC GAGCGGGTGT TCCTTCTTCA CTGTCCCTTA
     2761 TTCGCACCTG GCGGTGCTCA ACGGGAATCC TGCTCTGCGA GGCTGGCCGT AGGCCGGCCC
     2821 TACCGGCGCG GCAGCGTTAC CCGTGTCGGC GGCTCCAACG GCTCGCCATC GTCCAGAAAA
     2881 CACGGCTCAT CGGGCATCGG CAGGCGCTGC TGCCCGCGCC GTTCCCATTC CTCCGTTTCG
     2941 GTCAAGGCTG GCAGGTCTGG TTCCATGCCC GGAATGCCGG GCTGGCTGGG CGGCTCCTCG
     3001 CCGGGGCCGG TCGGTAGTTG CTGCTCGCCC GGATACAGGG TCGGGATGCG GCGCAGGTCG
     3061 CCATGCCCCA ACAGCGATTC GTCCTGGTCG TCGTGATCAA CCACCACGGC GGCACTGAAC
     3121 ACCGACAGGC GCAACTGGTC GCGGGGCTGG CCCCACGCCA CGCGGTCATT GACCACGTAG
     3181 GCCGACACGG TGCCGGGGCC GTTGAGCTTC ACGACGGAGA TCCAGCGCTC GGCCACCAAG
     3241 TCCTTGACTG CGTATTGGAC CGTCCGCAAA GAACGTCCGA TGAGCTTGGA AAGTCTCTTC
     3301 TGGCTGACCA CCACGGCGTT CTGGTGGCCC ATCTGCGCCA CGAGGTGATG CAGCAGCATT
     3361 GCCGCCGTGG GTTTCCTCGC AATAAGCCCG GCCCACGCCT CATGCGCTTT GCGTTCCGTT
     3421 TGCACCCAGT GACCGGGCTT GTTCTTGGCT TGAATGCCGA TTTCTCTGGA CTGCGTGGCC
     3481 ATGCTTATCT CCATGCGGTA GGGGTGCCGC ACGGTTGCGG CACCATGCGC AATCAGCTGC
     3541 AACTTTTCGG CAGCGCGACA ACAATTATGC GTTGCGTAAA AGTGGCAGTC AATTACAGAT
     3601 TTTCTTTAAC CTACGCAATG AGCTATTGCG GGGGGTGCCG CAATGAGCTG TTGCGTACCC
     3661 CCCTTTTTTA AGTTGTTGAT TTTTAAGTCT TTCGCATTTC GCCCTATATC TAGTTCTTTG
     3721 GTGCCCAAAG AAGGGCACCC CTGCGGGGTT CCCCCACGCC TTCGGCGCGG CTCCCCCTCC
     3781 GGCAAAAAGT GGCCCCTCCG GGGCTTGTTG ATCGACTGCG CGGCCTTCGG CCTTGCCCAA
     3841 GGTGGCGCTG CCCCCTTGGA ACCCCCGCAC TCGCCGCCGT GAGGCTCGGG GGGCAGGCGG
     3901 GCGGGCTTCG CCCTTCGACT GCCCCCACTC GCATAGGCTT GGGTCGTTCC AGGCGCGTCA
     3961 AGGCCAAGCC GCTGCGCGGT CGCTGCGCGA GCCTTGACCC GCCTTCCACT TGGTGTCCAA
     4021 CCGGCAAGCG AAGCGCGCAG GCCGCAGGCC GGAGGCTTTT CCCCAGAGAA AATTAAAAAA
     4081 ATTGATGGGG CAAGGCCGCA GGCCGCGCAG TTGGAGCCGG TGGGTATGTG GTCGAAGGCT
     4141 GGGTAGCCGG TGGGCAATCC CTGTGGTCAA GCTCGTGGGC AGGCGCAGCC TGTCCATCAG
     4201 CTTGTCCAGC AGGGTTGTCC ACGGGCCGAG CGAAGCGAGC CAGCCGGTGG CCGCTCGCGG
     4261 CCATCGTCCA CATATCCACG GGCTGGCAAG GGAGCGCAGC GACCGCGCAG GGCGAAGCCC
     4321 GGAGAGCAAG CCCGTAGGGG GGGCGCGCCC AGCTGTCTAG GGCGGCGGAT TTGTCCTACT
     4381 CAGGAGAGCG TTCACCGACA AACAACAGAT AAAACGAAAG GCCCAGTCTT TCGACTGAGC
     4441 CTTTCGTTTT ATTTGATGCC T   
//
