LOCUS       Untitled_Sequence        3197 bp    DNA     circular SYN 28-FEB-2023
FEATURES             Location/Qualifiers
     promoter        301..395
                     /label="pTRC"
                     /color="#ff0000"
     CDS             1049..1840
                     /label="AmpR"
                     /color="#ccffcc"
     rep_origin      2174..2762
                     /label="ori"
                     /color="#ffff00"
     oriT            2030..2139
                     /label="oriT"
                     /color="#a6acb3"
     terminator      721..815
                     /label="lambda t0 terminator"
                     /color="#ffffff"
     promoter        888..979
                     /label="AmpR promoter"
                     /color="#ffffff"
     terminator      3105..3191
                     /label="rrnB T1 terminator"
                     /color="#ffffff"
     terminator      1844..1883
                     /label="fd terminator"
                     /color="#ffffff"
     promoter        185..191
                     /label="lac promoter"
                     /color="#ffffff"
     promoter        356..362
                     /label="trc promoter"
                     /color="#ffffff"
     protein_bind    125..146
                     /label="CAP binding site"
                     /color="#31849b"
     protein_bind    3050..3071
                     /label="CAP binding site"
                     /color="#31849b"
     protein_bind    199..215
                     /label="lac operator"
                     /color="#31849b"
     primer_bind     223..239
                     /label="M13 rev"
                     /color="#a020f0"
     protein_bind    370..386
                     /label="lac operator"
                     /color="#31849b"
     primer_bind     426..442
                     /label="M13 fwd"
                     /color="#a020f0"
     misc_feature    396..399
                     /label="B"
                     /color="#00ffff"
     misc_feature    297..300
                     /label="A"
                     /color="#00ffff"
ORIGIN      
        1 TTAATTAAGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA
       61 TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG
      121 CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG
      181 CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC AGCTATGACC
      241 ATGATTACGC CAAGCTTGCA TGCCTGCAGG TCGACTCTAG AGGATCCCCG GTCTCAGGAG
      301 AACGGTTCTG GCAAATATTC TGAAATGAGC TGTTGACAAT TAATCATCCG GCTCGTATAA
      361 TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACATACTA GAGACCGGGT ACCGAGCTCG
      421 AATTCACTGG CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT
      481 AATCGCCTTG CAGCACATCC CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC
      541 GATCGCCCTT CCCAACAGTT GCGCAGCCTG AATGGCGAAT GGCGCCTGAT GCGGTATTTT
      601 CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATGGT GCACTCTCAG TACAATCTGC
      661 TCTGATGCCG CATAGTTAAG CCAGCCCCGA CACCCGCCAA CACCCGCTGA ACTAGTCTTG
      721 GACTCCTGTT GATAGATCCA GTAATGACCT CAGAACTCCA TCTGGATTTG TTCAGAACGC
      781 TCGGTTGCCG CCGGGCGTTT TTTATTGGTG AGAATCCAGG GGTCCCCAAT AATTACGATT
      841 TAAATTAGTA GCCCGCCTAA TGAGCGGGCT TTTTTTTAAT TCCCCTATTT GTTTATTTTT
      901 CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA
      961 ATATTGAAAA AGGAAGAGTA TGAGCATTCA GCATTTTCGT GTGGCGCTGA TTCCGTTTTT
     1021 TGCGGCGTTT TGCCTGCCGG TGTTTGCGCA TCCGGAAACC CTGGTGAAAG TGAAAGATGC
     1081 GGAAGATCAA CTGGGTGCGC GCGTGGGCTA TATTGAACTG GATCTGAACA GCGGCAAAAT
     1141 TCTGGAATCT TTTCGTCCGG AAGAACGTTT TCCGATGATG AGCACCTTTA AAGTGCTGCT
     1201 GTGCGGTGCG GTTCTGAGCC GTGTGGATGC GGGCCAGGAA CAACTGGGCC GTCGTATTCA
     1261 TTATAGCCAG AACGATCTGG TGGAATATAG CCCGGTGACC GAAAAACATC TGACCGATGG
     1321 CATGACCGTG CGTGAACTGT GCAGCGCGGC GATTACCATG AGCGATAACA CCGCGGCGAA
     1381 CCTGCTGCTG ACGACCATTG GCGGTCCGAA AGAACTGACC GCGTTTCTGC ATAACATGGG
     1441 CGATCATGTG ACCCGTCTGG ATCGTTGGGA ACCGGAACTG AACGAAGCGA TTCCGAACGA
     1501 TGAACGTGAT ACCACCATGC CGGCAGCAAT GGCGACCACC CTGCGTAAAC TGCTGACGGG
     1561 TGAGCTGCTG ACCCTGGCAA GCCGCCAGCA ACTGATTGAT TGGATGGAAG CGGATAAAGT
     1621 GGCGGGTCCG CTGCTGCGTA GCGCGCTGCC GGCTGGCTGG TTTATTGCGG ATAAAAGCGG
     1681 TGCGGGCGAA CGTGGCAGCC GTGGCATTAT TGCGGCGCTG GGCCCGGATG GTAAACCGAG
     1741 CCGTATTGTG GTGATTTATA CCACCGGCAG CCAGGCGACG ATGGATGAAC GTAACCGTCA
     1801 GATTGCGGAA ATTGGCGCGA GCCTGATTAA ACATTGGTAA ACCGATACAA TTAAAGGCTC
     1861 CTTTTGGAGC CTTTTTTTTT GGACGACCCT TGTCCTTTTC CGCTGCATAA CCCTGCTTCG
     1921 GGGTCATTAT AGCGATTTTT TCGGTATATC CATCCTTTTT CGCACGATAT ACAGGATTTT
     1981 GCCAAAGGGT TCGTGTAGAC TTTCCTTGGT GTATCCAACG GCGTCAGCCG GGCAGGATAG
     2041 GTGAAGTAGG CCCACCCGCG AGCGGGTGTT CCTTCTTCAC TGTCCCTTAT TCGCACCTGG
     2101 CGGTGCTCAA CGGGAATCCT GCTCTGCGAG GCTGGCCGTA GGCCGGCCCC GTAGAAAAGA
     2161 TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA
     2221 AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA
     2281 AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT
     2341 TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT
     2401 TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT
     2461 AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT
     2521 TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTTTGA GAAAGCGCCA
     2581 CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG
     2641 AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC
     2701 GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA
     2761 AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA
     2821 TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG
     2881 CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG
     2941 AAGAGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGATTCAT TAATGCAGCT
     3001 GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG CAACGCAATT AATGTGAGTT
     3061 AGCTCACTCA TTAGGCAGGC GCGCCCAGCT GTCTAGGGCG GCGGATTTGT CCTACTCAGG
     3121 AGAGCGTTCA CCGACAAACA ACAGATAAAA CGAAAGGCCC AGTCTTTCGA CTGAGCCTTT
     3181 CGTTTTATTT GATGCCT    
//
