LOCUS       p14        3092 bp DNA     circular SYN 13-DEC-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    p14_pSEVA182-J23102_BD
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 26, 2023 from SnapGene Viewer 6.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3092
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    125..146
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        161..191
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     204..227
                     /label=R24
     primer_bind     223..239
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             235..258
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPS"
     misc_feature    266..269
                     /label=A
     promoter        270..304
                     /label=J23102_AB
     misc_feature    305..308
                     /label=B
     primer_bind     complement(321..337)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(341..364)
                     /label=F24
     terminator      616..710
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        783..874
                     /gene="bla"
                     /label=AmpR promoter
     CDS             875..1735
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1739..1778
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            1925..2033
                     /note="incP origin of transfer"
     rep_origin      2069..2657
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2945..2966
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      3000..3086
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaaga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
       61 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
      121 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
      181 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
      241 atgattacgc caagctgagg tctcaggagt tgacagctag ctcagtccta ggtactgtgc
      301 tagctactag agaccaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
      361 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
      421 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
      481 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact
      541 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
      601 gctgaactag tcttggactc ctgttgatag atccagtaat gacctcagaa ctccatctgg
      661 atttgttcag aacgctcggt tgccgccggg cgttttttat tggtgagaat ccaggggtcc
      721 ccaataatta cgatttaaat tagtagcccg cctaatgagc gggctttttt ttaattcccc
      781 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      841 ataaatgctt caataatatt gaaaaaggaa gagtatgagc attcagcatt ttcgtgtggc
      901 gctgattccg ttttttgcgg cgttttgcct gccggtgttt gcgcatccgg aaaccctggt
      961 gaaagtgaaa gatgcggaag atcaactggg tgcgcgcgtg ggctatattg aactggatct
     1021 gaacagcggc aaaattctgg aatcttttcg tccggaagaa cgttttccga tgatgagcac
     1081 ctttaaagtg ctgctgtgcg gtgcggttct gagccgtgtg gatgcgggcc aggaacaact
     1141 gggccgtcgt attcattata gccagaacga tctggtggaa tatagcccgg tgaccgaaaa
     1201 acatctgacc gatggcatga ccgtgcgtga actgtgcagc gcggcgatta ccatgagcga
     1261 taacaccgcg gcgaacctgc tgctgacgac cattggcggt ccgaaagaac tgaccgcgtt
     1321 tctgcataac atgggcgatc atgtgacccg tctggatcgt tgggaaccgg aactgaacga
     1381 agcgattccg aacgatgaac gtgataccac catgccggca gcaatggcga ccaccctgcg
     1441 taaactgctg acgggtgagc tgctgaccct ggcaagccgc cagcaactga ttgattggat
     1501 ggaagcggat aaagtggcgg gtccgctgct gcgtagcgcg ctgccggctg gctggtttat
     1561 tgcggataaa agcggtgcgg gcgaacgtgg cagccgtggc attattgcgg cgctgggccc
     1621 ggatggtaaa ccgagccgta ttgtggtgat ttataccacc ggcagccagg cgacgatgga
     1681 tgaacgtaac cgtcagattg cggaaattgg cgcgagcctg attaaacatt ggtaaaccga
     1741 tacaattaaa ggctcctttt ggagcctttt tttttggacg acccttgtcc ttttccgctg
     1801 cataaccctg cttcggggtc attatagcga ttttttcggt atatccatcc tttttcgcac
     1861 gatatacagg attttgccaa agggttcgtg tagactttcc ttggtgtatc caacggcgtc
     1921 agccgggcag gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc ttcactgtcc
     1981 cttattcgca cctggcggtg ctcaacggga atcctgctct gcgaggctgg ccgtaggccg
     2041 gccccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     2101 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     2161 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     2221 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     2281 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     2341 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     2401 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     2461 tttgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     2521 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     2581 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     2641 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     2701 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     2761 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     2821 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     2881 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     2941 caattaatgt gagttagctc actcattagg caggcgcgcc cagctgtcta gggcggcgga
     3001 tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct
     3061 ttcgactgag cctttcgttt tatttgatgc ct
//
