LOCUS       Untitled_Sequence        3151 bp    DNA     circular SYN 28-FEB-2023
FEATURES             Location/Qualifiers
     primer_bind     380..396
                     /label="M13 fwd"
                     /color="#a020f0"
     primer_bind     223..239
                     /label="M13 rev"
                     /color="#a020f0"
     protein_bind    199..215
                     /label="lac operator"
                     /color="#31849b"
     promoter        301..319
                     /label="T7 promoter"
                     /color="#ffffff"
     protein_bind    3004..3025
                     /label="CAP binding site"
                     /color="#31849b"
     protein_bind    125..146
                     /label="CAP binding site"
                     /color="#31849b"
     protein_bind    320..344
                     /label="lac operator"
                     /color="#31849b"
     promoter        185..191
                     /label="lac promoter"
                     /color="#ffffff"
     terminator      1798..1837
                     /label="fd terminator"
                     /color="#ffffff"
     terminator      3059..3145
                     /label="rrnB T1 terminator"
                     /color="#ffffff"
     promoter        842..933
                     /label="AmpR promoter"
                     /color="#ffffff"
     terminator      675..769
                     /label="lambda t0 terminator"
                     /color="#ffffff"
     oriT            1984..2092
                     /label="oriT"
                     /color="#a6acb3"
     rep_origin      2128..2716
                     /label="ori"
                     /color="#ffff00"
     CDS             1003..1794
                     /label="AmpR"
                     /color="#ccffcc"
     misc_feature    297..300
                     /label="A"
                     /color="#00ffff"
     misc_feature    350..353
                     /label="B"
                     /color="#00ffff"
     promoter        301..349
                     /label="pT7_LacO"
                     /color="#ff0000"
ORIGIN      
        1 TTAATTAAGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA
       61 TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG
      121 CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG
      181 CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC AGCTATGACC
      241 ATGATTACGC CAAGCTTGCA TGCCTGCAGG TCGACTCTAG AGGATCCCCG GTCTCAGGAG
      301 TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA TTCCCCTCTT ACTAGAGACC
      361 GGGTACCGAG CTCGAATTCA CTGGCCGTCG TTTTACAACG TCGTGACTGG GAAAACCCTG
      421 GCGTTACCCA ACTTAATCGC CTTGCAGCAC ATCCCCCTTT CGCCAGCTGG CGTAATAGCG
      481 AAGAGGCCCG CACCGATCGC CCTTCCCAAC AGTTGCGCAG CCTGAATGGC GAATGGCGCC
      541 TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TGGTGCACTC
      601 TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGCC CCGACACCCG CCAACACCCG
      661 CTGAACTAGT CTTGGACTCC TGTTGATAGA TCCAGTAATG ACCTCAGAAC TCCATCTGGA
      721 TTTGTTCAGA ACGCTCGGTT GCCGCCGGGC GTTTTTTATT GGTGAGAATC CAGGGGTCCC
      781 CAATAATTAC GATTTAAATT AGTAGCCCGC CTAATGAGCG GGCTTTTTTT TAATTCCCCT
      841 ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA
      901 TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGCA TTCAGCATTT TCGTGTGGCG
      961 CTGATTCCGT TTTTTGCGGC GTTTTGCCTG CCGGTGTTTG CGCATCCGGA AACCCTGGTG
     1021 AAAGTGAAAG ATGCGGAAGA TCAACTGGGT GCGCGCGTGG GCTATATTGA ACTGGATCTG
     1081 AACAGCGGCA AAATTCTGGA ATCTTTTCGT CCGGAAGAAC GTTTTCCGAT GATGAGCACC
     1141 TTTAAAGTGC TGCTGTGCGG TGCGGTTCTG AGCCGTGTGG ATGCGGGCCA GGAACAACTG
     1201 GGCCGTCGTA TTCATTATAG CCAGAACGAT CTGGTGGAAT ATAGCCCGGT GACCGAAAAA
     1261 CATCTGACCG ATGGCATGAC CGTGCGTGAA CTGTGCAGCG CGGCGATTAC CATGAGCGAT
     1321 AACACCGCGG CGAACCTGCT GCTGACGACC ATTGGCGGTC CGAAAGAACT GACCGCGTTT
     1381 CTGCATAACA TGGGCGATCA TGTGACCCGT CTGGATCGTT GGGAACCGGA ACTGAACGAA
     1441 GCGATTCCGA ACGATGAACG TGATACCACC ATGCCGGCAG CAATGGCGAC CACCCTGCGT
     1501 AAACTGCTGA CGGGTGAGCT GCTGACCCTG GCAAGCCGCC AGCAACTGAT TGATTGGATG
     1561 GAAGCGGATA AAGTGGCGGG TCCGCTGCTG CGTAGCGCGC TGCCGGCTGG CTGGTTTATT
     1621 GCGGATAAAA GCGGTGCGGG CGAACGTGGC AGCCGTGGCA TTATTGCGGC GCTGGGCCCG
     1681 GATGGTAAAC CGAGCCGTAT TGTGGTGATT TATACCACCG GCAGCCAGGC GACGATGGAT
     1741 GAACGTAACC GTCAGATTGC GGAAATTGGC GCGAGCCTGA TTAAACATTG GTAAACCGAT
     1801 ACAATTAAAG GCTCCTTTTG GAGCCTTTTT TTTTGGACGA CCCTTGTCCT TTTCCGCTGC
     1861 ATAACCCTGC TTCGGGGTCA TTATAGCGAT TTTTTCGGTA TATCCATCCT TTTTCGCACG
     1921 ATATACAGGA TTTTGCCAAA GGGTTCGTGT AGACTTTCCT TGGTGTATCC AACGGCGTCA
     1981 GCCGGGCAGG ATAGGTGAAG TAGGCCCACC CGCGAGCGGG TGTTCCTTCT TCACTGTCCC
     2041 TTATTCGCAC CTGGCGGTGC TCAACGGGAA TCCTGCTCTG CGAGGCTGGC CGTAGGCCGG
     2101 CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG
     2161 CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC
     2221 AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTTCTTCT
     2281 AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC
     2341 TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT
     2401 GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG
     2461 CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT
     2521 TTGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG
     2581 GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG
     2641 TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG
     2701 GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG
     2761 GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC
     2821 CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT
     2881 GAGCGAGGAA GCGGAAGAGC GCCCAATACG CAAACCGCCT CTCCCCGCGC GTTGGCCGAT
     2941 TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA GCGGGCAGTG AGCGCAACGC
     3001 AATTAATGTG AGTTAGCTCA CTCATTAGGC AGGCGCGCCC AGCTGTCTAG GGCGGCGGAT
     3061 TTGTCCTACT CAGGAGAGCG TTCACCGACA AACAACAGAT AAAACGAAAG GCCCAGTCTT
     3121 TCGACTGAGC CTTTCGTTTT ATTTGATGCC T  
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