LOCUS       Untitled_Sequence        3170 bp    DNA     circular SYN 28-FEB-2023
FEATURES             Location/Qualifiers
     CDS             1022..1813
                     /label="AmpR"
                     /color="#ccffcc"
     rep_origin      2147..2735
                     /label="ori"
                     /color="#ffff00"
     oriT            2003..2112
                     /label="oriT"
                     /color="#a6acb3"
     terminator      694..788
                     /label="lambda t0 terminator"
                     /color="#ffffff"
     promoter        861..952
                     /label="AmpR promoter"
                     /color="#ffffff"
     terminator      3078..3164
                     /label="rrnB T1 terminator"
                     /color="#ffffff"
     promoter        303..350
                     /label="EM7 promoter"
                     /color="#ff0000"
     terminator      1817..1856
                     /label="fd terminator"
                     /color="#ffffff"
     promoter        185..191
                     /label="lac promoter"
                     /color="#ffffff"
     protein_bind    125..146
                     /label="CAP binding site"
                     /color="#31849b"
     protein_bind    3023..3044
                     /label="CAP binding site"
                     /color="#31849b"
     protein_bind    199..215
                     /label="lac operator"
                     /color="#31849b"
     primer_bind     223..239
                     /label="M13 rev"
                     /color="#a020f0"
     primer_bind     399..415
                     /label="M13 fwd"
                     /color="#a020f0"
     misc_feature    297..300
                     /label="A"
                     /color="#00ffff"
     misc_feature    369..372
                     /label="B"
                     /color="#00ffff"
ORIGIN      
        1 TTAATTAAGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA
       61 TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG
      121 CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG
      181 CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC AGCTATGACC
      241 ATGATTACGC CAAGCTTGCA TGCCTGCAGG TCGACTCTAG AGGATCCCCG GTCTCAGGAG
      301 TTGTTGACAA TTAATCATCG GCATAGTATA TCGGCATAGT ATAATACGAC AAGGTGAGGA
      361 ACTAAACCTA CTAGAGACCG GGTACCGAGC TCGAATTCAC TGGCCGTCGT TTTACAACGT
      421 CGTGACTGGG AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA TCCCCCTTTC
      481 GCCAGCTGGC GTAATAGCGA AGAGGCCCGC ACCGATCGCC CTTCCCAACA GTTGCGCAGC
      541 CTGAATGGCG AATGGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA
      601 CACCGCATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGCCC
      661 CGACACCCGC CAACACCCGC TGAACTAGTC TTGGACTCCT GTTGATAGAT CCAGTAATGA
      721 CCTCAGAACT CCATCTGGAT TTGTTCAGAA CGCTCGGTTG CCGCCGGGCG TTTTTTATTG
      781 GTGAGAATCC AGGGGTCCCC AATAATTACG ATTTAAATTA GTAGCCCGCC TAATGAGCGG
      841 GCTTTTTTTT AATTCCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT
      901 CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGCAT
      961 TCAGCATTTT CGTGTGGCGC TGATTCCGTT TTTTGCGGCG TTTTGCCTGC CGGTGTTTGC
     1021 GCATCCGGAA ACCCTGGTGA AAGTGAAAGA TGCGGAAGAT CAACTGGGTG CGCGCGTGGG
     1081 CTATATTGAA CTGGATCTGA ACAGCGGCAA AATTCTGGAA TCTTTTCGTC CGGAAGAACG
     1141 TTTTCCGATG ATGAGCACCT TTAAAGTGCT GCTGTGCGGT GCGGTTCTGA GCCGTGTGGA
     1201 TGCGGGCCAG GAACAACTGG GCCGTCGTAT TCATTATAGC CAGAACGATC TGGTGGAATA
     1261 TAGCCCGGTG ACCGAAAAAC ATCTGACCGA TGGCATGACC GTGCGTGAAC TGTGCAGCGC
     1321 GGCGATTACC ATGAGCGATA ACACCGCGGC GAACCTGCTG CTGACGACCA TTGGCGGTCC
     1381 GAAAGAACTG ACCGCGTTTC TGCATAACAT GGGCGATCAT GTGACCCGTC TGGATCGTTG
     1441 GGAACCGGAA CTGAACGAAG CGATTCCGAA CGATGAACGT GATACCACCA TGCCGGCAGC
     1501 AATGGCGACC ACCCTGCGTA AACTGCTGAC GGGTGAGCTG CTGACCCTGG CAAGCCGCCA
     1561 GCAACTGATT GATTGGATGG AAGCGGATAA AGTGGCGGGT CCGCTGCTGC GTAGCGCGCT
     1621 GCCGGCTGGC TGGTTTATTG CGGATAAAAG CGGTGCGGGC GAACGTGGCA GCCGTGGCAT
     1681 TATTGCGGCG CTGGGCCCGG ATGGTAAACC GAGCCGTATT GTGGTGATTT ATACCACCGG
     1741 CAGCCAGGCG ACGATGGATG AACGTAACCG TCAGATTGCG GAAATTGGCG CGAGCCTGAT
     1801 TAAACATTGG TAAACCGATA CAATTAAAGG CTCCTTTTGG AGCCTTTTTT TTTGGACGAC
     1861 CCTTGTCCTT TTCCGCTGCA TAACCCTGCT TCGGGGTCAT TATAGCGATT TTTTCGGTAT
     1921 ATCCATCCTT TTTCGCACGA TATACAGGAT TTTGCCAAAG GGTTCGTGTA GACTTTCCTT
     1981 GGTGTATCCA ACGGCGTCAG CCGGGCAGGA TAGGTGAAGT AGGCCCACCC GCGAGCGGGT
     2041 GTTCCTTCTT CACTGTCCCT TATTCGCACC TGGCGGTGCT CAACGGGAAT CCTGCTCTGC
     2101 GAGGCTGGCC GTAGGCCGGC Cccgtagaaa agatcaaagg atcttcttga gatccttttt
     2161 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     2221 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     2281 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     2341 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     2401 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     2461 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     2521 gatacctaca gcgtgagctt tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     2581 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     2641 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     2701 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     2761 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     2821 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     2881 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     2941 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     3001 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca GGCGCGCCCA
     3061 GCTGTCTAGG GCGGCGGATT TGTCCTACTC AGGAGAGCGT TCACCGACAA ACAACAGATA
     3121 AAACGAAAGG CCCAGTCTTT CGACTGAGCC TTTCGTTTTA TTTGATGCCT 
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