LOCUS       Untitled_Sequence        3143 bp    DNA     circular SYN 28-FEB-2023
FEATURES             Location/Qualifiers
     CDS             995..1786
                     /label="AmpR"
                     /color="#ccffcc"
     rep_origin      2120..2708
                     /label="ori"
                     /color="#ffff00"
     oriT            1976..2085
                     /label="oriT"
                     /color="#a6acb3"
     terminator      667..761
                     /label="lambda t0 terminator"
                     /color="#ffffff"
     promoter        834..925
                     /label="AmpR promoter"
                     /color="#ffffff"
     terminator      3051..3137
                     /label="rrnB T1 terminator"
                     /color="#ffffff"
     terminator      1790..1829
                     /label="fd terminator"
                     /color="#ffffff"
     promoter        185..191
                     /label="lac promoter"
                     /color="#ffffff"
     protein_bind    125..146
                     /label="CAP binding site"
                     /color="#31849b"
     protein_bind    2996..3017
                     /label="CAP binding site"
                     /color="#31849b"
     protein_bind    199..215
                     /label="lac operator"
                     /color="#31849b"
     primer_bind     223..239
                     /label="M13 rev"
                     /color="#a020f0"
     primer_bind     372..388
                     /label="M13 fwd"
                     /color="#a020f0"
     misc_feature    297..300
                     /label="A"
                     /color="#00ffff"
     misc_feature    342..345
                     /label="B"
                     /color="#00ffff"
     promoter        301..341
                     /label="BG17"
                     /color="#ff0000"
     -35_signal      306..311
                     /label="-35"
                     /color="#a6acb3"
     -10_signal      329..334
                     /label="-10"
                     /color="#a6acb3"
ORIGIN      
        1 TTAATTAAGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA
       61 TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG
      121 CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG
      181 CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC AGCTATGACC
      241 ATGATTACGC CAAGCTTGCA TGCCTGCAGG TCGACTCTAG AGGATCCCCG GTCTCAGGAG
      301 CGGAGTTGAC AACACTCGAA AAGCCGAGTA TAATCAGATG ATACTAGAGA CCGGGTACCG
      361 AGCTCGAATT CACTGGCCGT CGTTTTACAA CGTCGTGACT GGGAAAACCC TGGCGTTACC
      421 CAACTTAATC GCCTTGCAGC ACATCCCCCT TTCGCCAGCT GGCGTAATAG CGAAGAGGCC
      481 CGCACCGATC GCCCTTCCCA ACAGTTGCGC AGCCTGAATG GCGAATGGCG CCTGATGCGG
      541 TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATGGTGCAC TCTCAGTACA
      601 ATCTGCTCTG ATGCCGCATA GTTAAGCCAG CCCCGACACC CGCCAACACC CGCTGAACTA
      661 GTCTTGGACT CCTGTTGATA GATCCAGTAA TGACCTCAGA ACTCCATCTG GATTTGTTCA
      721 GAACGCTCGG TTGCCGCCGG GCGTTTTTTA TTGGTGAGAA TCCAGGGGTC CCCAATAATT
      781 ACGATTTAAA TTAGTAGCCC GCCTAATGAG CGGGCTTTTT TTTAATTCCC CTATTTGTTT
      841 ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT
      901 TCAATAATAT TGAAAAAGGA AGAGTATGAG CATTCAGCAT TTTCGTGTGG CGCTGATTCC
      961 GTTTTTTGCG GCGTTTTGCC TGCCGGTGTT TGCGCATCCG GAAACCCTGG TGAAAGTGAA
     1021 AGATGCGGAA GATCAACTGG GTGCGCGCGT GGGCTATATT GAACTGGATC TGAACAGCGG
     1081 CAAAATTCTG GAATCTTTTC GTCCGGAAGA ACGTTTTCCG ATGATGAGCA CCTTTAAAGT
     1141 GCTGCTGTGC GGTGCGGTTC TGAGCCGTGT GGATGCGGGC CAGGAACAAC TGGGCCGTCG
     1201 TATTCATTAT AGCCAGAACG ATCTGGTGGA ATATAGCCCG GTGACCGAAA AACATCTGAC
     1261 CGATGGCATG ACCGTGCGTG AACTGTGCAG CGCGGCGATT ACCATGAGCG ATAACACCGC
     1321 GGCGAACCTG CTGCTGACGA CCATTGGCGG TCCGAAAGAA CTGACCGCGT TTCTGCATAA
     1381 CATGGGCGAT CATGTGACCC GTCTGGATCG TTGGGAACCG GAACTGAACG AAGCGATTCC
     1441 GAACGATGAA CGTGATACCA CCATGCCGGC AGCAATGGCG ACCACCCTGC GTAAACTGCT
     1501 GACGGGTGAG CTGCTGACCC TGGCAAGCCG CCAGCAACTG ATTGATTGGA TGGAAGCGGA
     1561 TAAAGTGGCG GGTCCGCTGC TGCGTAGCGC GCTGCCGGCT GGCTGGTTTA TTGCGGATAA
     1621 AAGCGGTGCG GGCGAACGTG GCAGCCGTGG CATTATTGCG GCGCTGGGCC CGGATGGTAA
     1681 ACCGAGCCGT ATTGTGGTGA TTTATACCAC CGGCAGCCAG GCGACGATGG ATGAACGTAA
     1741 CCGTCAGATT GCGGAAATTG GCGCGAGCCT GATTAAACAT TGGTAAACCG ATACAATTAA
     1801 AGGCTCCTTT TGGAGCCTTT TTTTTTGGAC GACCCTTGTC CTTTTCCGCT GCATAACCCT
     1861 GCTTCGGGGT CATTATAGCG ATTTTTTCGG TATATCCATC CTTTTTCGCA CGATATACAG
     1921 GATTTTGCCA AAGGGTTCGT GTAGACTTTC CTTGGTGTAT CCAACGGCGT CAGCCGGGCA
     1981 GGATAGGTGA AGTAGGCCCA CCCGCGAGCG GGTGTTCCTT CTTCACTGTC CCTTATTCGC
     2041 ACCTGGCGGT GCTCAACGGG AATCCTGCTC TGCGAGGCTG GCCGTAGGCC GGCCccgtag
     2101 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     2161 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     2221 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     2281 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     2341 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     2401 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     2461 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctttgagaaa
     2521 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     2581 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     2641 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     2701 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     2761 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     2821 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     2881 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     2941 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     3001 tgagttagct cactcattag gcaGGCGCGC CCAGCTGTCT AGGGCGGCGG ATTTGTCCTA
     3061 CTCAGGAGAG CGTTCACCGA CAAACAACAG ATAAAACGAA AGGCCCAGTC TTTCGACTGA
     3121 GCCTTTCGTT TTATTTGATG CCT   
//