LOCUS       r03        3123 bp DNA     circular SYN 18-JUL-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 26, 2023 from SnapGene Viewer 6.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3123
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    125..146
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        161..191
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    199..215
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     204..227
                     /label=R24
     primer_bind     223..239
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    297..300
                     /label=D
     RBS             complement(301..321)
                     /label=B0034_BD
     RBS             306..317
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    322..325
                     /label=B
     primer_bind     complement(352..368)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(372..395)
                     /label=F24
     terminator      647..741
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        814..905
                     /gene="bla"
                     /label=AmpR promoter
     CDS             906..1766
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1770..1809
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            1956..2064
                     /note="incP origin of transfer"
     rep_origin      2100..2688
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2976..2997
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      3031..3117
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaaga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
       61 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
      121 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
      181 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
      241 atgattacgc caagcttgca tgcctgcagg tcgactctag aggatccccg gtctctcatt
      301 tagtatttct cctctttctc tagtatgaga ccgggtaccg agctcgaatt cactggccgt
      361 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
      421 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
      481 acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatct
      541 gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata
      601 gttaagccag ccccgacacc cgccaacacc cgctgaacta gtcttggact cctgttgata
      661 gatccagtaa tgacctcaga actccatctg gatttgttca gaacgctcgg ttgccgccgg
      721 gcgtttttta ttggtgagaa tccaggggtc cccaataatt acgatttaaa ttagtagccc
      781 gcctaatgag cgggcttttt tttaattccc ctatttgttt atttttctaa atacattcaa
      841 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      901 agagtatgag cattcagcat tttcgtgtgg cgctgattcc gttttttgcg gcgttttgcc
      961 tgccggtgtt tgcgcatccg gaaaccctgg tgaaagtgaa agatgcggaa gatcaactgg
     1021 gtgcgcgcgt gggctatatt gaactggatc tgaacagcgg caaaattctg gaatcttttc
     1081 gtccggaaga acgttttccg atgatgagca cctttaaagt gctgctgtgc ggtgcggttc
     1141 tgagccgtgt ggatgcgggc caggaacaac tgggccgtcg tattcattat agccagaacg
     1201 atctggtgga atatagcccg gtgaccgaaa aacatctgac cgatggcatg accgtgcgtg
     1261 aactgtgcag cgcggcgatt accatgagcg ataacaccgc ggcgaacctg ctgctgacga
     1321 ccattggcgg tccgaaagaa ctgaccgcgt ttctgcataa catgggcgat catgtgaccc
     1381 gtctggatcg ttgggaaccg gaactgaacg aagcgattcc gaacgatgaa cgtgatacca
     1441 ccatgccggc agcaatggcg accaccctgc gtaaactgct gacgggtgag ctgctgaccc
     1501 tggcaagccg ccagcaactg attgattgga tggaagcgga taaagtggcg ggtccgctgc
     1561 tgcgtagcgc gctgccggct ggctggttta ttgcggataa aagcggtgcg ggcgaacgtg
     1621 gcagccgtgg cattattgcg gcgctgggcc cggatggtaa accgagccgt attgtggtga
     1681 tttataccac cggcagccag gcgacgatgg atgaacgtaa ccgtcagatt gcggaaattg
     1741 gcgcgagcct gattaaacat tggtaaaccg atacaattaa aggctccttt tggagccttt
     1801 ttttttggac gacccttgtc cttttccgct gcataaccct gcttcggggt cattatagcg
     1861 attttttcgg tatatccatc ctttttcgca cgatatacag gattttgcca aagggttcgt
     1921 gtagactttc cttggtgtat ccaacggcgt cagccgggca ggataggtga agtaggccca
     1981 cccgcgagcg ggtgttcctt cttcactgtc ccttattcgc acctggcggt gctcaacggg
     2041 aatcctgctc tgcgaggctg gccgtaggcc ggccccgtag aaaagatcaa aggatcttct
     2101 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     2161 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     2221 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     2281 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     2341 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     2401 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     2461 tacaccgaac tgagatacct acagcgtgag ctttgagaaa gcgccacgct tcccgaaggg
     2521 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     2581 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     2641 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     2701 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     2761 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     2821 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     2881 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     2941 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     3001 gcaggcgcgc ccagctgtct agggcggcgg atttgtccta ctcaggagag cgttcaccga
     3061 caaacaacag ataaaacgaa aggcccagtc tttcgactga gcctttcgtt ttatttgatg
     3121 cct
//
