LOCUS       r05        3186 bp DNA     circular SYN 26-JUL-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 26, 2023 from SnapGene Viewer 6.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3186
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    125..146
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        161..191
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    199..215
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     204..227
                     /label=R24
     primer_bind     223..239
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    297..300
                     /label=D
     RBS             complement(301..384)
                     /label=BCD2
     RBS             306..314
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     misc_feature    385..388
                     /label=B
     primer_bind     complement(415..431)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(435..458)
                     /label=F24
     terminator      710..804
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        877..968
                     /gene="bla"
                     /label=AmpR promoter
     CDS             969..1829
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1833..1872
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            2019..2127
                     /note="incP origin of transfer"
     rep_origin      2163..2751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3039..3060
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      3094..3180
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaaga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
       61 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
      121 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
      181 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
      241 atgattacgc caagcttgca tgcctgcagg tcgactctag aggatccccg gtctctcatt
      301 agaaaacctc cttagcatga ttaagatgtt tcagtacgaa aattgctttc attgttgatc
      361 tcctttttaa gtgaacttgg gcccagtatg agaccgggta ccgagctcga attcactggc
      421 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
      481 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
      541 ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca
      601 tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc
      661 atagttaagc cagccccgac acccgccaac acccgctgaa ctagtcttgg actcctgttg
      721 atagatccag taatgacctc agaactccat ctggatttgt tcagaacgct cggttgccgc
      781 cgggcgtttt ttattggtga gaatccaggg gtccccaata attacgattt aaattagtag
      841 cccgcctaat gagcgggctt ttttttaatt cccctatttg tttatttttc taaatacatt
      901 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      961 ggaagagtat gagcattcag cattttcgtg tggcgctgat tccgtttttt gcggcgtttt
     1021 gcctgccggt gtttgcgcat ccggaaaccc tggtgaaagt gaaagatgcg gaagatcaac
     1081 tgggtgcgcg cgtgggctat attgaactgg atctgaacag cggcaaaatt ctggaatctt
     1141 ttcgtccgga agaacgtttt ccgatgatga gcacctttaa agtgctgctg tgcggtgcgg
     1201 ttctgagccg tgtggatgcg ggccaggaac aactgggccg tcgtattcat tatagccaga
     1261 acgatctggt ggaatatagc ccggtgaccg aaaaacatct gaccgatggc atgaccgtgc
     1321 gtgaactgtg cagcgcggcg attaccatga gcgataacac cgcggcgaac ctgctgctga
     1381 cgaccattgg cggtccgaaa gaactgaccg cgtttctgca taacatgggc gatcatgtga
     1441 cccgtctgga tcgttgggaa ccggaactga acgaagcgat tccgaacgat gaacgtgata
     1501 ccaccatgcc ggcagcaatg gcgaccaccc tgcgtaaact gctgacgggt gagctgctga
     1561 ccctggcaag ccgccagcaa ctgattgatt ggatggaagc ggataaagtg gcgggtccgc
     1621 tgctgcgtag cgcgctgccg gctggctggt ttattgcgga taaaagcggt gcgggcgaac
     1681 gtggcagccg tggcattatt gcggcgctgg gcccggatgg taaaccgagc cgtattgtgg
     1741 tgatttatac caccggcagc caggcgacga tggatgaacg taaccgtcag attgcggaaa
     1801 ttggcgcgag cctgattaaa cattggtaaa ccgatacaat taaaggctcc ttttggagcc
     1861 tttttttttg gacgaccctt gtccttttcc gctgcataac cctgcttcgg ggtcattata
     1921 gcgatttttt cggtatatcc atcctttttc gcacgatata caggattttg ccaaagggtt
     1981 cgtgtagact ttccttggtg tatccaacgg cgtcagccgg gcaggatagg tgaagtaggc
     2041 ccacccgcga gcgggtgttc cttcttcact gtcccttatt cgcacctggc ggtgctcaac
     2101 gggaatcctg ctctgcgagg ctggccgtag gccggccccg tagaaaagat caaaggatct
     2161 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     2221 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     2281 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     2341 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     2401 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     2461 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     2521 acctacaccg aactgagata cctacagcgt gagctttgag aaagcgccac gcttcccgaa
     2581 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     2641 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     2701 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     2761 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     2821 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     2881 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     2941 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     3001 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     3061 taggcaggcg cgcccagctg tctagggcgg cggatttgtc ctactcagga gagcgttcac
     3121 cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc gttttatttg
     3181 atgcct
//
