LOCUS       Untitled_Sequence        3120 bp    DNA     circular SYN 28-FEB-2023
FEATURES             Location/Qualifiers
     RBS             306..314
                     /label="Shine-Dalgarno sequence"
                     /color="#a6acb3"
     primer_bind     349..365
                     /label="M13 fwd"
                     /color="#a020f0"
     primer_bind     223..239
                     /label="M13 rev"
                     /color="#a020f0"
     protein_bind    199..215
                     /label="lac operator"
                     /color="#31849b"
     protein_bind    2973..2994
                     /label="CAP binding site"
                     /color="#31849b"
     protein_bind    125..146
                     /label="CAP binding site"
                     /color="#31849b"
     promoter        185..191
                     /label="lac promoter"
                     /color="#ffffff"
     terminator      1767..1806
                     /label="fd terminator"
                     /color="#ffffff"
     terminator      3028..3114
                     /label="rrnB T1 terminator"
                     /color="#ffffff"
     promoter        811..902
                     /label="AmpR promoter"
                     /color="#ffffff"
     terminator      644..738
                     /label="lambda t0 terminator"
                     /color="#ffffff"
     oriT            1953..2061
                     /label="oriT"
                     /color="#a6acb3"
     rep_origin      2097..2685
                     /label="ori"
                     /color="#ffff00"
     CDS             972..1763
                     /label="AmpR"
                     /color="#ccffcc"
     misc_feature    297..300
                     /label="B"
                     /color="#00ffff"
     misc_feature    319..322
                     /label="D"
                     /color="#00ffff"
     promoter        301..318
                     /label="RBS-ST"
                     /color="#ff0000"
ORIGIN      
        1 TTAATTAAGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA
       61 TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG
      121 CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG
      181 CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC AGCTATGACC
      241 ATGATTACGC CAAGCTTGCA TGCCTGCAGG TCGACTCTAG AGGATCCCCG GTCTCTTACT
      301 TGACCTAAGG AGGTAAATAA TGAGAGACCG GGTACCGAGC TCGAATTCAC TGGCCGTCGT
      361 TTTACAACGT CGTGACTGGG AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA
      421 TCCCCCTTTC GCCAGCTGGC GTAATAGCGA AGAGGCCCGC ACCGATCGCC CTTCCCAACA
      481 GTTGCGCAGC CTGAATGGCG AATGGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG
      541 CGGTATTTCA CACCGCATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT
      601 AAGCCAGCCC CGACACCCGC CAACACCCGC TGAACTAGTC TTGGACTCCT GTTGATAGAT
      661 CCAGTAATGA CCTCAGAACT CCATCTGGAT TTGTTCAGAA CGCTCGGTTG CCGCCGGGCG
      721 TTTTTTATTG GTGAGAATCC AGGGGTCCCC AATAATTACG ATTTAAATTA GTAGCCCGCC
      781 TAATGAGCGG GCTTTTTTTT AATTCCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA
      841 TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA
      901 GTATGAGCAT TCAGCATTTT CGTGTGGCGC TGATTCCGTT TTTTGCGGCG TTTTGCCTGC
      961 CGGTGTTTGC GCATCCGGAA ACCCTGGTGA AAGTGAAAGA TGCGGAAGAT CAACTGGGTG
     1021 CGCGCGTGGG CTATATTGAA CTGGATCTGA ACAGCGGCAA AATTCTGGAA TCTTTTCGTC
     1081 CGGAAGAACG TTTTCCGATG ATGAGCACCT TTAAAGTGCT GCTGTGCGGT GCGGTTCTGA
     1141 GCCGTGTGGA TGCGGGCCAG GAACAACTGG GCCGTCGTAT TCATTATAGC CAGAACGATC
     1201 TGGTGGAATA TAGCCCGGTG ACCGAAAAAC ATCTGACCGA TGGCATGACC GTGCGTGAAC
     1261 TGTGCAGCGC GGCGATTACC ATGAGCGATA ACACCGCGGC GAACCTGCTG CTGACGACCA
     1321 TTGGCGGTCC GAAAGAACTG ACCGCGTTTC TGCATAACAT GGGCGATCAT GTGACCCGTC
     1381 TGGATCGTTG GGAACCGGAA CTGAACGAAG CGATTCCGAA CGATGAACGT GATACCACCA
     1441 TGCCGGCAGC AATGGCGACC ACCCTGCGTA AACTGCTGAC GGGTGAGCTG CTGACCCTGG
     1501 CAAGCCGCCA GCAACTGATT GATTGGATGG AAGCGGATAA AGTGGCGGGT CCGCTGCTGC
     1561 GTAGCGCGCT GCCGGCTGGC TGGTTTATTG CGGATAAAAG CGGTGCGGGC GAACGTGGCA
     1621 GCCGTGGCAT TATTGCGGCG CTGGGCCCGG ATGGTAAACC GAGCCGTATT GTGGTGATTT
     1681 ATACCACCGG CAGCCAGGCG ACGATGGATG AACGTAACCG TCAGATTGCG GAAATTGGCG
     1741 CGAGCCTGAT TAAACATTGG TAAACCGATA CAATTAAAGG CTCCTTTTGG AGCCTTTTTT
     1801 TTTGGACGAC CCTTGTCCTT TTCCGCTGCA TAACCCTGCT TCGGGGTCAT TATAGCGATT
     1861 TTTTCGGTAT ATCCATCCTT TTTCGCACGA TATACAGGAT TTTGCCAAAG GGTTCGTGTA
     1921 GACTTTCCTT GGTGTATCCA ACGGCGTCAG CCGGGCAGGA TAGGTGAAGT AGGCCCACCC
     1981 GCGAGCGGGT GTTCCTTCTT CACTGTCCCT TATTCGCACC TGGCGGTGCT CAACGGGAAT
     2041 CCTGCTCTGC GAGGCTGGCC GTAGGCCGGC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA
     2101 GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG
     2161 GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC
     2221 AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG
     2281 AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC
     2341 AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG
     2401 CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC
     2461 ACCGAACTGA GATACCTACA GCGTGAGCTT TGAGAAAGCG CCACGCTTCC CGAAGGGAGA
     2521 AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT
     2581 CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG
     2641 CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG
     2701 GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA
     2761 TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC
     2821 AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC
     2881 AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC
     2941 GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA
     3001 GGCGCGCCCA GCTGTCTAGG GCGGCGGATT TGTCCTACTC AGGAGAGCGT TCACCGACAA
     3061 ACAACAGATA AAACGAAAGG CCCAGTCTTT CGACTGAGCC TTTCGTTTTA TTTGATGCCT
//
