LOCUS       Untitled_Sequence        3135 bp    DNA     circular SYN 28-FEB-2023
FEATURES             Location/Qualifiers
     CDS             987..1778
                     /label="AmpR"
                     /color="#ccffcc"
     rep_origin      2112..2700
                     /label="ori"
                     /color="#ffff00"
     oriT            1968..2077
                     /label="oriT"
                     /color="#a6acb3"
     terminator      659..753
                     /label="lambda t0 terminator"
                     /color="#ffffff"
     promoter        826..917
                     /label="AmpR promoter"
                     /color="#ffffff"
     terminator      3043..3129
                     /label="rrnB T1 terminator"
                     /color="#ffffff"
     terminator      1782..1821
                     /label="fd terminator"
                     /color="#ffffff"
     promoter        185..191
                     /label="lac promoter"
                     /color="#ffffff"
     RBS             306..328
                     /label="RBS"
                     /color="#a6acb3"
     protein_bind    125..146
                     /label="CAP binding site"
                     /color="#31849b"
     protein_bind    2988..3009
                     /label="CAP binding site"
                     /color="#31849b"
     protein_bind    199..215
                     /label="lac operator"
                     /color="#31849b"
     primer_bind     223..239
                     /label="M13 rev"
                     /color="#a020f0"
     primer_bind     364..380
                     /label="M13 fwd"
                     /color="#a020f0"
     misc_feature    297..300
                     /label="B"
                     /color="#00ffff"
     misc_feature    334..337
                     /label="D"
                     /color="#00ffff"
     misc_feature    301..333
                     /label="T7-RBS"
                     /color="#ff0000"
ORIGIN      
        1 TTAATTAAGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA
       61 TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG
      121 CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG
      181 CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC AGCTATGACC
      241 ATGATTACGC CAAGCTTGCA TGCCTGCAGG TCGACTCTAG AGGATCCCCG GTCTCATACT
      301 ATAATTTTGT TTAACTTTAA GAAGGAGATA TACAATGAGA GACCGGGTAC CGAGCTCGAA
      361 TTCACTGGCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC CCTGGCGTTA CCCAACTTAA
      421 TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA
      481 TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG CGCCTGATGC GGTATTTTCT
      541 CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATGGTGC ACTCTCAGTA CAATCTGCTC
      601 TGATGCCGCA TAGTTAAGCC AGCCCCGACA CCCGCCAACA CCCGCTGAAC TAGTCTTGGA
      661 CTCCTGTTGA TAGATCCAGT AATGACCTCA GAACTCCATC TGGATTTGTT CAGAACGCTC
      721 GGTTGCCGCC GGGCGTTTTT TATTGGTGAG AATCCAGGGG TCCCCAATAA TTACGATTTA
      781 AATTAGTAGC CCGCCTAATG AGCGGGCTTT TTTTTAATTC CCCTATTTGT TTATTTTTCT
      841 AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT
      901 ATTGAAAAAG GAAGAGTATG AGCATTCAGC ATTTTCGTGT GGCGCTGATT CCGTTTTTTG
      961 CGGCGTTTTG CCTGCCGGTG TTTGCGCATC CGGAAACCCT GGTGAAAGTG AAAGATGCGG
     1021 AAGATCAACT GGGTGCGCGC GTGGGCTATA TTGAACTGGA TCTGAACAGC GGCAAAATTC
     1081 TGGAATCTTT TCGTCCGGAA GAACGTTTTC CGATGATGAG CACCTTTAAA GTGCTGCTGT
     1141 GCGGTGCGGT TCTGAGCCGT GTGGATGCGG GCCAGGAACA ACTGGGCCGT CGTATTCATT
     1201 ATAGCCAGAA CGATCTGGTG GAATATAGCC CGGTGACCGA AAAACATCTG ACCGATGGCA
     1261 TGACCGTGCG TGAACTGTGC AGCGCGGCGA TTACCATGAG CGATAACACC GCGGCGAACC
     1321 TGCTGCTGAC GACCATTGGC GGTCCGAAAG AACTGACCGC GTTTCTGCAT AACATGGGCG
     1381 ATCATGTGAC CCGTCTGGAT CGTTGGGAAC CGGAACTGAA CGAAGCGATT CCGAACGATG
     1441 AACGTGATAC CACCATGCCG GCAGCAATGG CGACCACCCT GCGTAAACTG CTGACGGGTG
     1501 AGCTGCTGAC CCTGGCAAGC CGCCAGCAAC TGATTGATTG GATGGAAGCG GATAAAGTGG
     1561 CGGGTCCGCT GCTGCGTAGC GCGCTGCCGG CTGGCTGGTT TATTGCGGAT AAAAGCGGTG
     1621 CGGGCGAACG TGGCAGCCGT GGCATTATTG CGGCGCTGGG CCCGGATGGT AAACCGAGCC
     1681 GTATTGTGGT GATTTATACC ACCGGCAGCC AGGCGACGAT GGATGAACGT AACCGTCAGA
     1741 TTGCGGAAAT TGGCGCGAGC CTGATTAAAC ATTGGTAAAC CGATACAATT AAAGGCTCCT
     1801 TTTGGAGCCT TTTTTTTTGG ACGACCCTTG TCCTTTTCCG CTGCATAACC CTGCTTCGGG
     1861 GTCATTATAG CGATTTTTTC GGTATATCCA TCCTTTTTCG CACGATATAC AGGATTTTGC
     1921 CAAAGGGTTC GTGTAGACTT TCCTTGGTGT ATCCAACGGC GTCAGCCGGG CAGGATAGGT
     1981 GAAGTAGGCC CACCCGCGAG CGGGTGTTCC TTCTTCACTG TCCCTTATTC GCACCTGGCG
     2041 GTGCTCAACG GGAATCCTGC TCTGCGAGGC TGGCCGTAGG CCGGCCCCGT AGAAAAGATC
     2101 AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA
     2161 CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG
     2221 GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTTC TTCTAGTGTA GCCGTAGTTA
     2281 GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA
     2341 CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG
     2401 TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG
     2461 GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTTTGAGA AAGCGCCACG
     2521 CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG
     2581 CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC
     2641 CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA
     2701 AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG
     2761 TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT
     2821 GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA
     2881 GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG
     2941 CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTGAGTTAG
     3001 CTCACTCATT AGGCAGGCGC GCCCAGCTGT CTAGGGCGGC GGATTTGTCC TACTCAGGAG
     3061 AGCGTTCACC GACAAACAAC AGATAAAACG AAAGGCCCAG TCTTTCGACT GAGCCTTTCG
     3121 TTTTATTTGA TGCCT    
//
