LOCUS       Untitled_Sequence        3125 bp    DNA     circular SYN 28-FEB-2023
FEATURES             Location/Qualifiers
     primer_bind     354..370
                     /label="M13 fwd"
                     /color="#a020f0"
     primer_bind     223..239
                     /label="M13 rev"
                     /color="#a020f0"
     protein_bind    199..215
                     /label="lac operator"
                     /color="#31849b"
     CDS             303..320
                     /label="6xHis"
                     /color="#cc99b2"
     protein_bind    2978..2999
                     /label="CAP binding site"
                     /color="#31849b"
     protein_bind    125..146
                     /label="CAP binding site"
                     /color="#31849b"
     promoter        185..191
                     /label="lac promoter"
                     /color="#ffffff"
     terminator      1772..1811
                     /label="fd terminator"
                     /color="#ffffff"
     terminator      3033..3119
                     /label="rrnB T1 terminator"
                     /color="#ffffff"
     promoter        816..907
                     /label="AmpR promoter"
                     /color="#ffffff"
     terminator      649..743
                     /label="lambda t0 terminator"
                     /color="#ffffff"
     oriT            1958..2066
                     /label="oriT"
                     /color="#a6acb3"
     rep_origin      2102..2690
                     /label="ori"
                     /color="#ffff00"
     CDS             977..1768
                     /label="AmpR"
                     /color="#ccffcc"
     misc_feature    324..327
                     /label="G"
                     /color="#00ffff"
     misc_feature    297..300
                     /label="F"
                     /color="#00ffff"
     misc_feature    301..323
                     /label="C-6xHistag_FG"
                     /color="#ff0000"
ORIGIN      
        1 TTAATTAAGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA
       61 TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG
      121 CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG
      181 CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC AGCTATGACC
      241 ATGATTACGC CAAGCTTGCA TGCCTGCAGG TCGACTCTAG AGGATCCCCG GTCTCATTCG
      301 GCCATCATCA CCATCATCAC TAAGCTTAGA GACCGGGTAC CGAGCTCGAA TTCACTGGCC
      361 GTCGTTTTAC AACGTCGTGA CTGGGAAAAC CCTGGCGTTA CCCAACTTAA TCGCCTTGCA
      421 GCACATCCCC CTTTCGCCAG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA TCGCCCTTCC
      481 CAACAGTTGC GCAGCCTGAA TGGCGAATGG CGCCTGATGC GGTATTTTCT CCTTACGCAT
      541 CTGTGCGGTA TTTCACACCG CATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA
      601 TAGTTAAGCC AGCCCCGACA CCCGCCAACA CCCGCTGAAC TAGTCTTGGA CTCCTGTTGA
      661 TAGATCCAGT AATGACCTCA GAACTCCATC TGGATTTGTT CAGAACGCTC GGTTGCCGCC
      721 GGGCGTTTTT TATTGGTGAG AATCCAGGGG TCCCCAATAA TTACGATTTA AATTAGTAGC
      781 CCGCCTAATG AGCGGGCTTT TTTTTAATTC CCCTATTTGT TTATTTTTCT AAATACATTC
      841 AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG
      901 GAAGAGTATG AGCATTCAGC ATTTTCGTGT GGCGCTGATT CCGTTTTTTG CGGCGTTTTG
      961 CCTGCCGGTG TTTGCGCATC CGGAAACCCT GGTGAAAGTG AAAGATGCGG AAGATCAACT
     1021 GGGTGCGCGC GTGGGCTATA TTGAACTGGA TCTGAACAGC GGCAAAATTC TGGAATCTTT
     1081 TCGTCCGGAA GAACGTTTTC CGATGATGAG CACCTTTAAA GTGCTGCTGT GCGGTGCGGT
     1141 TCTGAGCCGT GTGGATGCGG GCCAGGAACA ACTGGGCCGT CGTATTCATT ATAGCCAGAA
     1201 CGATCTGGTG GAATATAGCC CGGTGACCGA AAAACATCTG ACCGATGGCA TGACCGTGCG
     1261 TGAACTGTGC AGCGCGGCGA TTACCATGAG CGATAACACC GCGGCGAACC TGCTGCTGAC
     1321 GACCATTGGC GGTCCGAAAG AACTGACCGC GTTTCTGCAT AACATGGGCG ATCATGTGAC
     1381 CCGTCTGGAT CGTTGGGAAC CGGAACTGAA CGAAGCGATT CCGAACGATG AACGTGATAC
     1441 CACCATGCCG GCAGCAATGG CGACCACCCT GCGTAAACTG CTGACGGGTG AGCTGCTGAC
     1501 CCTGGCAAGC CGCCAGCAAC TGATTGATTG GATGGAAGCG GATAAAGTGG CGGGTCCGCT
     1561 GCTGCGTAGC GCGCTGCCGG CTGGCTGGTT TATTGCGGAT AAAAGCGGTG CGGGCGAACG
     1621 TGGCAGCCGT GGCATTATTG CGGCGCTGGG CCCGGATGGT AAACCGAGCC GTATTGTGGT
     1681 GATTTATACC ACCGGCAGCC AGGCGACGAT GGATGAACGT AACCGTCAGA TTGCGGAAAT
     1741 TGGCGCGAGC CTGATTAAAC ATTGGTAAAC CGATACAATT AAAGGCTCCT TTTGGAGCCT
     1801 TTTTTTTTGG ACGACCCTTG TCCTTTTCCG CTGCATAACC CTGCTTCGGG GTCATTATAG
     1861 CGATTTTTTC GGTATATCCA TCCTTTTTCG CACGATATAC AGGATTTTGC CAAAGGGTTC
     1921 GTGTAGACTT TCCTTGGTGT ATCCAACGGC GTCAGCCGGG CAGGATAGGT GAAGTAGGCC
     1981 CACCCGCGAG CGGGTGTTCC TTCTTCACTG TCCCTTATTC GCACCTGGCG GTGCTCAACG
     2041 GGAATCCTGC TCTGCGAGGC TGGCCGTAGG CCGGCCCCGT AGAAAAGATC AAAGGATCTT
     2101 CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC
     2161 CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT
     2221 TCAGCAGAGC GCAGATACCA AATACTGTTC TTCTAGTGTA GCCGTAGTTA GGCCACCACT
     2281 TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG
     2341 CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA
     2401 AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA
     2461 CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTTTGAGA AAGCGCCACG CTTCCCGAAG
     2521 GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG
     2581 AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC
     2641 TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA
     2701 ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG
     2761 CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC
     2821 GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA
     2881 TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT
     2941 TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT
     3001 AGGCAGGCGC GCCCAGCTGT CTAGGGCGGC GGATTTGTCC TACTCAGGAG AGCGTTCACC
     3061 GACAAACAAC AGATAAAACG AAAGGCCCAG TCTTTCGACT GAGCCTTTCG TTTTATTTGA
     3121 TGCCT     
//