LOCUS       Untitled_Sequence        3132 bp    DNA     circular SYN 28-FEB-2023
FEATURES             Location/Qualifiers
     primer_bind     2756..2772
                     /label="M13 fwd"
                     /color="#a020f0"
     primer_bind     2894..2910
                     /label="M13 rev"
                     /color="#a020f0"
     protein_bind    127..148
                     /label="CAP binding site"
                     /color="#31849b"
     protein_bind    2987..3008
                     /label="CAP binding site"
                     /color="#31849b"
     promoter        2967..2972
                     /label="lac promoter"
                     /color="#ffffff"
     terminator      1315..1354
                     /label="fd terminator"
                     /color="#ffffff"
     terminator      7..93
                     /label="rrnB T1 terminator"
                     /color="#ffffff"
     promoter        2219..2310
                     /label="AmpR promoter"
                     /color="#ffffff"
     terminator      2383..2477
                     /label="lambda t0 terminator"
                     /color="#ffffff"
     oriT            1060..1168
                     /label="oriT"
                     /color="#a6acb3"
     rep_origin      436..1024
                     /label="ori"
                     /color="#ffff00"
     CDS             2150..2218
                     /label="AmpR"
                     /color="#ccffcc"
     CDS             2801..2827
                     /label="Strep-Tag II"
                     /color="#cc99b2"
     misc_feature    2799..2802
                     /label="C"
                     /color="#00ffff"
     misc_feature    2833..2836
                     /label="D"
                     /color="#00ffff"
     misc_feature    2803..2832
                     /label="N-Streptag_CD"
                     /color="#ff0000"
ORIGIN      
        1 AGGCATCAAA TAAAACGAAA GGCTCAGTCG AAAGACTGGG CCTTTCGTTT TATCTGTTGT
       61 TTGTCGGTGA ACGCTCTCCT GAGTAGGACA AATCCGCCGC CCTAGACAGC TGGGCGCGCC
      121 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
      181 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
      241 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
      301 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
      361 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      421 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
      481 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      541 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      601 tctcccttcg ggaagcgtgg cgctttctca aagctcacgc tgtaggtatc tcagttcggt
      661 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
      721 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
      781 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
      841 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
      901 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
      961 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     1021 tcaagaagat cctttgatct tttctacggG GCCGGCCTAC GGCCAGCCTC GCAGAGCAGG
     1081 ATTCCCGTTG AGCACCGCCA GGTGCGAATA AGGGACAGTG AAGAAGGAAC ACCCGCTCGC
     1141 GGGTGGGCCT ACTTCACCTA TCCTGCCCGG CTGACGCCGT TGGATACACC AAGGAAAGTC
     1201 TACACGAACC CTTTGGCAAA ATCCTGTATA TCGTGCGAAA AAGGATGGAT ATACCGAAAA
     1261 AATCGCTATA ATGACCCCGA AGCAGGGTTA TGCAGCGGAA AAGGACAAGG GTCGTCCAAA
     1321 AAAAAAGGCT CCAAAAGGAG CCTTTAATTG TATCGGTTTA CCAATGTTTA ATCAGGCTCG
     1381 CGCCAATTTC CGCAATCTGA CGGTTACGTT CATCCATCGT CGCCTGGCTG CCGGTGGTAT
     1441 AAATCACCAC AATACGGCTC GGTTTACCAT CCGGGCCCAG CGCCGCAATA ATGCCACGGC
     1501 TGCCACGTTC GCCCGCACCG CTTTTATCCG CAATAAACCA GCCAGCCGGC AGCGCGCTAC
     1561 GCAGCAGCGG ACCCGCCACT TTATCCGCTT CCATCCAATC AATCAGTTGC TGGCGGCTTG
     1621 CCAGGGTCAG CAGCTCACCC GTCAGCAGTT TACGCAGGGT GGTCGCCATT GCTGCCGGCA
     1681 TGGTGGTATC ACGTTCATCG TTCGGAATCG CTTCGTTCAG TTCCGGTTCC CAACGATCCA
     1741 GACGGGTCAC ATGATCGCCC ATGTTATGCA GAAACGCGGT CAGTTCTTTC GGACCGCCAA
     1801 TGGTCGTCAG CAGCAGGTTC GCCGCGGTGT TATCGCTCAT GGTAATCGCC GCGCTGCACA
     1861 GTTCACGCAC GGTCATGCCA TCGGTCAGAT GTTTTTCGGT CACCGGGCTA TATTCCACCA
     1921 GATCGTTCTG GCTATAATGA ATACGACGGC CCAGTTGTTC CTGGCCCGCA TCCACACGGC
     1981 TCAGAACCGC ACCGCACAGC AGCACTTTAA AGGTGCTCAT CATCGGAAAA CGTTCTTCCG
     2041 GACGAAAAGA TTCCAGAATT TTGCCGCTGT TCAGATCCAG TTCAATATAG CCCACGCGCG
     2101 CACCCAGTTG ATCTTCCGCA TCTTTCACTT TCACCAGGGT TTCCGGATGC GCAAACACCG
     2161 GCAGGCAAAA CGCCGCAAAA AACGGAATCA GCGCCACACG AAAATGCTGA ATGCTCATAC
     2221 TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA
     2281 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGAATT AAAAAAAAGC CCGCTCATTA
     2341 GGCGGGCTAC TAATTTAAAT CGTAATTATT GGGGACCCCT GGATTCTCAC CAATAAAAAA
     2401 CGCCCGGCGG CAACCGAGCG TTCTGAACAA ATCCAGATGG AGTTCTGAGG TCATTACTGG
     2461 ATCTATCAAC AGGAGTCCAA Gactagttca gcgggtgttg gcgggtgtcg gggctggctt
     2521 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
     2581 cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac
     2641 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
     2701 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
     2761 acgacggcca gtgaattcga gctcggtacc cGGTCTCACC ATgtggtccc atccgcagtt
     2821 tgaaaagggc gcAATGAGAG ACCggggatc ctctagagtc gacctgcagg catgcaagct
     2881 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     2941 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     3001 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     3061 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     3121 cttcttaatt aa    
//