LOCUS       Untitled_Sequence        3056 bp    DNA     circular SYN 28-FEB-2023
FEATURES             Location/Qualifiers
     CDS             2068..2928
                     /label="AmpR"
                     /color="#993366"
     rep_origin      1253..1920
                     /label="Col E1 origin"
                     /color="#ffff00"
     CDS             379..1032
                     /label="GST"
                     /color="#cc99b2"
     promoter        2929..3033
                     /label="AmpR promoter"
                     /color="#ffffff"
     CDS             1039..1062
                     /label="HRV 3C site"
                     /color="#cc99b2"
     promoter        320..338
                     /label="T7 promoter"
                     /color="#ffffff"
     primer_bind     293..309
                     /label="M13 fwd"
                     /color="#a020f0"
     misc_feature    377..380
                     /label="C"
                     /color="#00ffff"
     misc_feature    1074..1077
                     /label="D"
                     /color="#00ffff"
     misc_feature    381..1073
                     /label="N-GST HRV3C tag_CD"
                     /color="#ff0000"
ORIGIN      
        1 CTAAATTGTA AGCGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC
       61 ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA
      121 GATAGGGTTG AGTGGCCGCT ACAGGGCGCT CCCATTCGCC ATTCAGGCTG CGCAACTGTT
      181 GGGAAGGGCG TTTCGGTGCG GGCCTCTTCG CTATTACGCC AGCTGGCGAA AGGGGGATGT
      241 GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG
      301 ACGGCCAGTG AGCGCGACGT AATACGACTC ACTATAGGGC GAATTGGCGG AAGGCCGTCA
      361 AGGCCGCATG GTCTCACCAT GAGTCCGATT TTAGGTTACT GGAAAATCAA AGGTCTGGTT
      421 CAGCCGACAC GTCTGCTGCT GGAATATCTG GAAGAAAAAT ATGAGGAACA CCTGTATGAA
      481 CGCGACGAAG GTGATAAATG GCGTAACAAA AAATTCGAAC TGGGCCTTGA ATTTCCGAAT
      541 CTGCCGTATT ATATCGATGG TGATGTTAAA CTGACCCAGA GCATGGCAAT TATTCGTTAT
      601 ATTGCCGACA AGCATAACAT GCTTGGTGGT TGTCCGAAAG AACGTGCAGA AATTAGCATG
      661 CTGGAAGGTG CAGTTCTGGA TATTCGCTAT GGTGTTAGCC GTATTGCATA TAGCAAAGAT
      721 TTCGAAACCC TGAAAGTGGA CTTTCTGAGC AAACTGCCGG AAATGCTGAA AATGTTTGAA
      781 GATCGTCTGT GCCACAAAAC CTATCTGAAT GGTGATCATG TTACCCATCC GGATTTTATG
      841 CTGTATGACG CACTGGATGT TGTGCTGTAT ATGGATCCGA TGTGTCTGGA TGCATTTCCG
      901 AAACTGGTTT GCTTCAAAAA ACGCATTGAA GCCATTCCGC AGATCGACAA ATATCTGAAA
      961 AGCAGCAAAT ATATCGCATG GCCTCTGCAA GGTTGGCAGG CAACCTTTGG TGGTGGCGAT
     1021 CATCCGCCTA AAAGCGATCT GGAAGTTCTG TTTCAGGGTC CGCTGGGTAG CGCAATGAGA
     1081 GACCCTGGGC CTCATGGGCC TTCCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT
     1141 GCCAGCTGCA TTAACATGGT CATAGCTGTT TCCTTGCGTA TTGGGCGCTC TCCGCTTCCT
     1201 CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGGTAAAGCC TGGGGTGCCT AATGAGCAAA
     1261 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT
     1321 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC
     1381 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC
     1441 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC
     1501 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG
     1561 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA
     1621 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG
     1681 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA
     1741 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG
     1801 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG
     1861 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC
     1921 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC
     1981 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG
     2041 TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC
     2101 AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC
     2161 GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG AACCACGCTC
     2221 ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG
     2281 TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG
     2341 TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC
     2401 ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC
     2461 ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG
     2521 AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC
     2581 TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG
     2641 AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC
     2701 GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT
     2761 CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG
     2821 ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA
     2881 TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT
     2941 TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG
     3001 TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCAC
//