LOCUS       Untitled_Sequence        3839 bp    DNA     circular SYN 28-FEB-2023
FEATURES             Location/Qualifiers
     primer_bind     2756..2772
                     /label="M13 fwd"
                     /color="#a020f0"
     primer_bind     3601..3617
                     /label="M13 rev"
                     /color="#a020f0"
     protein_bind    127..148
                     /label="CAP binding site"
                     /color="#31849b"
     protein_bind    3694..3715
                     /label="CAP binding site"
                     /color="#31849b"
     promoter        3674..3679
                     /label="lac promoter"
                     /color="#ffffff"
     terminator      1315..1354
                     /label="fd terminator"
                     /color="#ffffff"
     terminator      7..93
                     /label="rrnB T1 terminator"
                     /color="#ffffff"
     promoter        2219..2310
                     /label="AmpR promoter"
                     /color="#ffffff"
     terminator      2383..2477
                     /label="lambda t0 terminator"
                     /color="#ffffff"
     oriT            1060..1168
                     /label="oriT"
                     /color="#a6acb3"
     rep_origin      436..1024
                     /label="ori"
                     /color="#ffff00"
     CDS             2150..2218
                     /label="AmpR"
                     /color="#ccffcc"
     CDS             2820..3536
                     /label="GFP"
                     /color="#05fd14"
     misc_feature    3540..3543
                     /label="G"
                     /color="#00ffff"
     misc_feature    2799..2802
                     /label="F"
                     /color="#00ffff"
     misc_feature    2803..3539
                     /label="C-LinkGFP_FG"
                     /color="#ff0000"
ORIGIN      
        1 AGGCATCAAA TAAAACGAAA GGCTCAGTCG AAAGACTGGG CCTTTCGTTT TATCTGTTGT
       61 TTGTCGGTGA ACGCTCTCCT GAGTAGGACA AATCCGCCGC CCTAGACAGC TGGGCGCGCC
      121 TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC
      181 GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT
      241 GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT
      301 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA
      361 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC
      421 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG
      481 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG
      541 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT
      601 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA AAGCTCACGC TGTAGGTATC TCAGTTCGGT
      661 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG
      721 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT
      781 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT
      841 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT
      901 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC
      961 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC
     1021 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GCCGGCCTAC GGCCAGCCTC GCAGAGCAGG
     1081 ATTCCCGTTG AGCACCGCCA GGTGCGAATA AGGGACAGTG AAGAAGGAAC ACCCGCTCGC
     1141 GGGTGGGCCT ACTTCACCTA TCCTGCCCGG CTGACGCCGT TGGATACACC AAGGAAAGTC
     1201 TACACGAACC CTTTGGCAAA ATCCTGTATA TCGTGCGAAA AAGGATGGAT ATACCGAAAA
     1261 AATCGCTATA ATGACCCCGA AGCAGGGTTA TGCAGCGGAA AAGGACAAGG GTCGTCCAAA
     1321 AAAAAAGGCT CCAAAAGGAG CCTTTAATTG TATCGGTTTA CCAATGTTTA ATCAGGCTCG
     1381 CGCCAATTTC CGCAATCTGA CGGTTACGTT CATCCATCGT CGCCTGGCTG CCGGTGGTAT
     1441 AAATCACCAC AATACGGCTC GGTTTACCAT CCGGGCCCAG CGCCGCAATA ATGCCACGGC
     1501 TGCCACGTTC GCCCGCACCG CTTTTATCCG CAATAAACCA GCCAGCCGGC AGCGCGCTAC
     1561 GCAGCAGCGG ACCCGCCACT TTATCCGCTT CCATCCAATC AATCAGTTGC TGGCGGCTTG
     1621 CCAGGGTCAG CAGCTCACCC GTCAGCAGTT TACGCAGGGT GGTCGCCATT GCTGCCGGCA
     1681 TGGTGGTATC ACGTTCATCG TTCGGAATCG CTTCGTTCAG TTCCGGTTCC CAACGATCCA
     1741 GACGGGTCAC ATGATCGCCC ATGTTATGCA GAAACGCGGT CAGTTCTTTC GGACCGCCAA
     1801 TGGTCGTCAG CAGCAGGTTC GCCGCGGTGT TATCGCTCAT GGTAATCGCC GCGCTGCACA
     1861 GTTCACGCAC GGTCATGCCA TCGGTCAGAT GTTTTTCGGT CACCGGGCTA TATTCCACCA
     1921 GATCGTTCTG GCTATAATGA ATACGACGGC CCAGTTGTTC CTGGCCCGCA TCCACACGGC
     1981 TCAGAACCGC ACCGCACAGC AGCACTTTAA AGGTGCTCAT CATCGGAAAA CGTTCTTCCG
     2041 GACGAAAAGA TTCCAGAATT TTGCCGCTGT TCAGATCCAG TTCAATATAG CCCACGCGCG
     2101 CACCCAGTTG ATCTTCCGCA TCTTTCACTT TCACCAGGGT TTCCGGATGC GCAAACACCG
     2161 GCAGGCAAAA CGCCGCAAAA AACGGAATCA GCGCCACACG AAAATGCTGA ATGCTCATAC
     2221 TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA
     2281 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGAATT AAAAAAAAGC CCGCTCATTA
     2341 GGCGGGCTAC TAATTTAAAT CGTAATTATT GGGGACCCCT GGATTCTCAC CAATAAAAAA
     2401 CGCCCGGCGG CAACCGAGCG TTCTGAACAA ATCCAGATGG AGTTCTGAGG TCATTACTGG
     2461 ATCTATCAAC AGGAGTCCAA GACTAGTTCA GCGGGTGTTG GCGGGTGTCG GGGCTGGCTT
     2521 AACTATGCGG CATCAGAGCA GATTGTACTG AGAGTGCACC ATATGCGGTG TGAAATACCG
     2581 CACAGATGCG TAAGGAGAAA ATACCGCATC AGGCGCCATT CGCCATTCAG GCTGCGCAAC
     2641 TGTTGGGAAG GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA
     2701 TGTGCTGCAA GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA
     2761 ACGACGGCCA GTGAATTCGA GCTCGGTACC CGGTCTCATT CGGTACCAGC GCGGCGGCGA
     2821 TGCGTAAAGG AGAAGAACTT TTCACTGGAG TTGTCCCAAT TCTTGTTGAA TTAGATGGTG
     2881 ATGTTAATGG GCACAAATTT TCTGTCAGTG GAGAGGGTGA AGGTGATGCA ACATACGGAA
     2941 AACTTACCCT TAAATTTATT TGCACTACTG GAAAACTACC TGTTCCATGG CCAACACTTG
     3001 TCACTACTTT CGGTTATGGT GTTCAATGCT TTGCGAGATA CCCAGATCAT ATGAAACAGC
     3061 ATGACTTTTT CAAGAGTGCC ATGCCCGAAG GTTATGTACA GGAAAGAACT ATATTTTTCA
     3121 AAGATGACGG GAACTACAAG ACACGTGCTG AAGTCAAGTT TGAAGGTGAT ACCCTTGTTA
     3181 ATAGAATCGA GTTAAAAGGT ATTGATTTTA AAGAAGATGG AAACATTCTT GGACACAAAT
     3241 TGGAATACAA CTATAACTCA CACAATGTAT ACATCATGGC AGACAAACAA AAGAATGGAA
     3301 TCAAAGTTAA CTTCAAAATT AGACACAACA TTGAAGATGG AAGCGTTCAA CTAGCAGACC
     3361 ATTATCAACA AAATACTCCA ATTGGCGATG GCCCTGTCCT TTTACCAGAC AACCATTACC
     3421 TGTCCACACA ATCTGCCCTT TCGAAAGATC CCAACGAAAA GAGAGATCAC ATGGTCCTTC
     3481 TTGAGTTTGT AACAGCTGCT GGGATTACAC ATGGCATGGA TGAACTATAC AAATAATAAG
     3541 CTTAGAGACC GGGGATCCTC TAGAGTCGAC CTGCAGGCAT GCAAGCTTGG CGTAATCATG
     3601 GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC
     3661 CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC
     3721 GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT
     3781 CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CTTAATTAA
//
