LOCUS       Untitled_Sequence        3165 bp    DNA     circular SYN 28-FEB-2023
FEATURES             Location/Qualifiers
     primer_bind     394..410
                     /label="M13 fwd"
                     /color="#a020f0"
     primer_bind     223..239
                     /label="M13 rev"
                     /color="#a020f0"
     protein_bind    3018..3039
                     /label="CAP binding site"
                     /color="#31849b"
     protein_bind    125..146
                     /label="CAP binding site"
                     /color="#31849b"
     promoter        185..191
                     /label="lac promoter"
                     /color="#ffffff"
     terminator      1812..1851
                     /label="fd terminator"
                     /color="#ffffff"
     terminator      3073..3159
                     /label="rrnB T1 terminator"
                     /color="#ffffff"
     promoter        856..947
                     /label="AmpR promoter"
                     /color="#ffffff"
     terminator      689..783
                     /label="lambda t0 terminator"
                     /color="#ffffff"
     oriT            1998..2106
                     /label="oriT"
                     /color="#a6acb3"
     rep_origin      2142..2730
                     /label="ori"
                     /color="#ffff00"
     CDS             1017..1808
                     /label="AmpR"
                     /color="#ccffcc"
     sig_peptide     299..364
                     /label="pelB signal sequence"
                     /color="#993366"
     misc_feature    297..300
                     /label="C"
                     /color="#00ffff"
     misc_feature    364..367
                     /label="D"
                     /color="#00ffff"
     misc_feature    301..363
                     /label="N-PelB_CD"
                     /color="#ff0000"
ORIGIN      
        1 ttaattaaga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
       61 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
      121 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
      181 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
      241 atgattacgc caagcttgca tgcctgcagg tcgactctag aggatccccG GTCTCACCAT
      301 gaaatacctg ctgccgaccg ctgctgctgg tctgctgctc ctcgctgccc agccggcgat
      361 ggcAATGAGA GACCgggtac cgagctcgaa ttcactggcc gtcgttttac aacgtcgtga
      421 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
      481 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
      541 tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
      601 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
      661 cccgccaaca cccgctgaac tagtCTTGGA CTCCTGTTGA TAGATCCAGT AATGACCTCA
      721 GAACTCCATC TGGATTTGTT CAGAACGCTC GGTTGCCGCC GGGCGTTTTT TATTGGTGAG
      781 AATCCAGGGG TCCCCAATAA TTACGATTTA AATTAGTAGC CCGCCTAATG AGCGGGCTTT
      841 TTTTTAATTC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA
      901 GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGCATTCAGC
      961 ATTTTCGTGT GGCGCTGATT CCGTTTTTTG CGGCGTTTTG CCTGCCGGTG TTTGCGCATC
     1021 CGGAAACCCT GGTGAAAGTG AAAGATGCGG AAGATCAACT GGGTGCGCGC GTGGGCTATA
     1081 TTGAACTGGA TCTGAACAGC GGCAAAATTC TGGAATCTTT TCGTCCGGAA GAACGTTTTC
     1141 CGATGATGAG CACCTTTAAA GTGCTGCTGT GCGGTGCGGT TCTGAGCCGT GTGGATGCGG
     1201 GCCAGGAACA ACTGGGCCGT CGTATTCATT ATAGCCAGAA CGATCTGGTG GAATATAGCC
     1261 CGGTGACCGA AAAACATCTG ACCGATGGCA TGACCGTGCG TGAACTGTGC AGCGCGGCGA
     1321 TTACCATGAG CGATAACACC GCGGCGAACC TGCTGCTGAC GACCATTGGC GGTCCGAAAG
     1381 AACTGACCGC GTTTCTGCAT AACATGGGCG ATCATGTGAC CCGTCTGGAT CGTTGGGAAC
     1441 CGGAACTGAA CGAAGCGATT CCGAACGATG AACGTGATAC CACCATGCCG GCAGCAATGG
     1501 CGACCACCCT GCGTAAACTG CTGACGGGTG AGCTGCTGAC CCTGGCAAGC CGCCAGCAAC
     1561 TGATTGATTG GATGGAAGCG GATAAAGTGG CGGGTCCGCT GCTGCGTAGC GCGCTGCCGG
     1621 CTGGCTGGTT TATTGCGGAT AAAAGCGGTG CGGGCGAACG TGGCAGCCGT GGCATTATTG
     1681 CGGCGCTGGG CCCGGATGGT AAACCGAGCC GTATTGTGGT GATTTATACC ACCGGCAGCC
     1741 AGGCGACGAT GGATGAACGT AACCGTCAGA TTGCGGAAAT TGGCGCGAGC CTGATTAAAC
     1801 ATTGGTAAAC CGATACAATT AAAGGCTCCT TTTGGAGCCT TTTTTTTTGG ACGACCCTTG
     1861 TCCTTTTCCG CTGCATAACC CTGCTTCGGG GTCATTATAG CGATTTTTTC GGTATATCCA
     1921 TCCTTTTTCG CACGATATAC AGGATTTTGC CAAAGGGTTC GTGTAGACTT TCCTTGGTGT
     1981 ATCCAACGGC GTCAGCCGGG CAGGATAGGT GAAGTAGGCC CACCCGCGAG CGGGTGTTCC
     2041 TTCTTCACTG TCCCTTATTC GCACCTGGCG GTGCTCAACG GGAATCCTGC TCTGCGAGGC
     2101 TGGCCGTAGG CCGGCCccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2161 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     2221 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     2281 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     2341 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     2401 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     2461 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     2521 ctacagcgtg agctttgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     2581 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     2641 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     2701 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     2761 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     2821 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     2881 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     2941 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     3001 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcaGGCGC GCCCAGCTGT
     3061 CTAGGGCGGC GGATTTGTCC TACTCAGGAG AGCGTTCACC GACAAACAAC AGATAAAACG
     3121 AAAGGCCCAG TCTTTCGACT GAGCCTTTCG TTTTATTTGA TGCCT 
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