LOCUS       Lv._0_StrepTag_GH       2685 bp ds-DNA     circular     02-FEB-2023
DEFINITION  .
KEYWORDS    "marker:AmpR"
FEATURES             Location/Qualifiers
     primer_bind     9..32
                     /label="R24"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
                     /note="note: R24"
     primer          9..32
                     /label="R24"
                     /note="sequence: agcggataacaatttcacacagga"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    82..85
                     /label="G"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             91..114
                     /label="Strep-Tag II"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="codon_start: 1 product: peptide that binds Strep-Tactin®, an engineered form of streptavidin transl_table: 1 translation: WSHPQFEK"
     misc_feature    118..121
                     /label="H"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer_bind     175..198
                     /label="F24"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
                     /note="note: F24"
     primer          complement(175..198)
                     /label="F24"
                     /note="sequence: CGCCAGGGTTTTCCCAGTCACGAC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     terminator      complement(205..307)
                     /label="T0 terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: T0 terminator"
     primer          complement(263..279)
                     /label="PS2"
                     /note="sequence: GCGGCAACCGAGCGTTC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     CDS             468..1328
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="label: AmpR"
     misc_feature    complement(1401..1605)
                     /label="OriT"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: OriT"
     rep_origin      complement(1667..2534)
                     /label="pUC Ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: LEFT label: pUC Ori"
     terminator      complement(2580..2684)
                     /label="T1 terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: T1 terminator"
     primer          2583..2600
                     /label="PS1"
                     /note="sequence: AGGGCGGCGGATTTGTCC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
ORIGIN
        1 TTAATTAAAG CGGATAACAA TTTCACACAG GAGGCCGCCT AGGCCGCGGC CGCGCGAATT
       61 CGAGCTCGGT ACCCGGTCTC GGCTTCGGGT TGGTCCCATC CGCAGTTTGA AAAGTAAGGT
      121 ATGAGACCGG GGATCCTCTA GAGTCGACCT GCAGGCATGC AAGCTTGCGG CCGCGTCGTG
      181 ACTGGGAAAA CCCTGGCGAC TAGTCTTGGA CTCCTGTTGA TAGATCCAGT AATGACCTCA
      241 GAACTCCATC TGGATTTGTT CAGAACGCTC GGTTGCCGCC GGGCGTTTTT TATTGGTGAG
      301 AATCCAGGGG TCCCCAATAA TTACGATTTA AATTAGTAGC CCGCCTAATG AGCGGGCTTT
      361 TTTTTAATTC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA
      421 GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGCATTCAGC
      481 ATTTTCGTGT GGCGCTGATT CCGTTTTTTG CGGCGTTTTG CCTGCCGGTG TTTGCGCATC
      541 CGGAAACCCT GGTGAAAGTG AAAGATGCGG AAGATCAACT GGGTGCGCGC GTGGGCTATA
      601 TTGAACTGGA TCTGAACAGC GGCAAAATTC TGGAATCTTT TCGTCCGGAA GAACGTTTTC
      661 CGATGATGAG CACCTTTAAA GTGCTGCTGT GCGGTGCGGT TCTGAGCCGT GTGGATGCGG
      721 GCCAGGAACA ACTGGGCCGT CGTATTCATT ATAGCCAGAA CGATCTGGTG GAATATAGCC
      781 CGGTGACCGA AAAACATCTG ACCGATGGCA TGACCGTGCG TGAACTGTGC AGCGCGGCGA
      841 TTACCATGAG CGATAACACC GCGGCGAACC TGCTGCTGAC GACCATTGGC GGTCCGAAAG
      901 AACTGACCGC GTTTCTGCAT AACATGGGCG ATCATGTGAC CCGTCTGGAT CGTTGGGAAC
      961 CGGAACTGAA CGAAGCGATT CCGAACGATG AACGTGATAC CACCATGCCG GCAGCAATGG
     1021 CGACCACCCT GCGTAAACTG CTGACGGGTG AGCTGCTGAC CCTGGCAAGC CGCCAGCAAC
     1081 TGATTGATTG GATGGAAGCG GATAAAGTGG CGGGTCCGCT GCTGCGTAGC GCGCTGCCGG
     1141 CTGGCTGGTT TATTGCGGAT AAAAGCGGTG CGGGCGAACG TGGCAGCCGT GGCATTATTG
     1201 CGGCGCTGGG CCCGGATGGT AAACCGAGCC GTATTGTGGT GATTTATACC ACCGGCAGCC
     1261 AGGCGACGAT GGATGAACGT AACCGTCAGA TTGCGGAAAT TGGCGCGAGC CTGATTAAAC
     1321 ATTGGTAAAC CGATACAATT AAAGGCTCCT TTTGGAGCCT TTTTTTTTGG ACGACCCTTG
     1381 TCCTTTTCCG CTGCATAACC CTGCTTCGGG GTCATTATAG CGATTTTTTC GGTATATCCA
     1441 TCCTTTTTCG CACGATATAC AGGATTTTGC CAAAGGGTTC GTGTAGACTT TCCTTGGTGT
     1501 ATCCAACGGC GTCAGCCGGG CAGGATAGGT GAAGTAGGCC CACCCGCGAG CGGGTGTTCC
     1561 TTCTTCACTG TCCCTTATTC GCACCTGGCG GTGCTCAACG GGAATCCTGC TCTGCGAGGC
     1621 TGGCCGTAGG CCGGCCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG
     1681 CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG
     1741 GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA
     1801 AATACTGTTC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG
     1861 CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG
     1921 TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA
     1981 ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC
     2041 CTACAGCGTG AGCTTTGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT
     2101 CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC
     2161 TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA
     2221 TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC
     2281 CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG
     2341 GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG
     2401 CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC
     2461 GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC
     2521 AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCAGGCGC GCCCAGCTGT
     2581 CTAGGGCGGC GGATTTGTCC TACTCAGGAG AGCGTTCACC GACAAACAAC AGATAAAACG
     2641 AAAGGCCCAG TCTTTCGACT GAGCCTTTCG TTTTATTTGA TGCCT
//