LOCUS       te11_BBa_B1006_H        3141 bp DNA     circular SYN 26-JUL-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   SBG
  TITLE     Direct Submission
  JOURNAL   Exported Sep 26, 2023 from SnapGene Viewer 6.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3141
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    125..146
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        161..191
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    199..215
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     223..239
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    297..300
                     /label=H
     terminator      301..339
                     /label=BBa_B1006
     misc_feature    340..343
                     /label=I
     primer_bind     complement(370..386)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      665..759
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        832..923
                     /gene="bla"
                     /label=AmpR promoter
     CDS             924..1784
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1788..1827
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            1974..2082
                     /note="incP origin of transfer"
     rep_origin      2118..2706
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2994..3015
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      3049..3135
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaaga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
       61 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
      121 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
      181 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
      241 atgattacgc caagcttgca tgcctgcagg tcgactctag aggatccccg gtctcaggta
      301 aaaaaaaaac cccgcccctg acagggcggg gttttttttc gcttgagacc gggtaccgag
      361 ctcgaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
      421 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
      481 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta
      541 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat
      601 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgaactagt
      661 cttggactcc tgttgataga tccagtaatg acctcagaac tccatctgga tttgttcaga
      721 acgctcggtt gccgccgggc gttttttatt ggtgagaatc caggggtccc caataattac
      781 gatttaaatt agtagcccgc ctaatgagcg ggcttttttt taattcccct atttgtttat
      841 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
      901 aataatattg aaaaaggaag agtatgagca ttcagcattt tcgtgtggcg ctgattccgt
      961 tttttgcggc gttttgcctg ccggtgtttg cgcatccgga aaccctggtg aaagtgaaag
     1021 atgcggaaga tcaactgggt gcgcgcgtgg gctatattga actggatctg aacagcggca
     1081 aaattctgga atcttttcgt ccggaagaac gttttccgat gatgagcacc tttaaagtgc
     1141 tgctgtgcgg tgcggttctg agccgtgtgg atgcgggcca ggaacaactg ggccgtcgta
     1201 ttcattatag ccagaacgat ctggtggaat atagcccggt gaccgaaaaa catctgaccg
     1261 atggcatgac cgtgcgtgaa ctgtgcagcg cggcgattac catgagcgat aacaccgcgg
     1321 cgaacctgct gctgacgacc attggcggtc cgaaagaact gaccgcgttt ctgcataaca
     1381 tgggcgatca tgtgacccgt ctggatcgtt gggaaccgga actgaacgaa gcgattccga
     1441 acgatgaacg tgataccacc atgccggcag caatggcgac caccctgcgt aaactgctga
     1501 cgggtgagct gctgaccctg gcaagccgcc agcaactgat tgattggatg gaagcggata
     1561 aagtggcggg tccgctgctg cgtagcgcgc tgccggctgg ctggtttatt gcggataaaa
     1621 gcggtgcggg cgaacgtggc agccgtggca ttattgcggc gctgggcccg gatggtaaac
     1681 cgagccgtat tgtggtgatt tataccaccg gcagccaggc gacgatggat gaacgtaacc
     1741 gtcagattgc ggaaattggc gcgagcctga ttaaacattg gtaaaccgat acaattaaag
     1801 gctccttttg gagccttttt ttttggacga cccttgtcct tttccgctgc ataaccctgc
     1861 ttcggggtca ttatagcgat tttttcggta tatccatcct ttttcgcacg atatacagga
     1921 ttttgccaaa gggttcgtgt agactttcct tggtgtatcc aacggcgtca gccgggcagg
     1981 ataggtgaag taggcccacc cgcgagcggg tgttccttct tcactgtccc ttattcgcac
     2041 ctggcggtgc tcaacgggaa tcctgctctg cgaggctggc cgtaggccgg ccccgtagaa
     2101 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     2161 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     2221 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     2281 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     2341 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     2401 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     2461 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct ttgagaaagc
     2521 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     2581 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     2641 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     2701 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     2761 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     2821 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     2881 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     2941 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     3001 agttagctca ctcattaggc aggcgcgccc agctgtctag ggcggcggat ttgtcctact
     3061 caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt tcgactgagc
     3121 ctttcgtttt atttgatgcc t
//
