LOCUS       Untitled_Sequence        3080 bp    DNA     circular SYN 28-FEB-2023
FEATURES             Location/Qualifiers
     CDS             932..1723
                     /label="AmpR"
                     /color="#ccffcc"
     rep_origin      2057..2645
                     /label="pUC"
                     /color="#ffff00"
     CDS             235..558
                     /label="lacZα"
                     /color="#993366"
     oriT            1913..2021
                     /label="oriT"
                     /color="#a6acb3"
     terminator      604..698
                     /label="lambda t0 terminator"
                     /color="#ffffff"
     promoter        771..862
                     /label="AmpR promoter"
                     /color="#ffffff"
     terminator      2988..3074
                     /label="rrnB T1 terminator"
                     /color="#ffffff"
     misc_feature    252..308
                     /label="MCS"
                     /color="#99ccff"
     terminator      1727..1766
                     /label="fd terminator"
                     /color="#ffffff"
     promoter        185..191
                     /label="lac promoter"
                     /color="#ffffff"
     protein_bind    125..146
                     /label="CAP binding site"
                     /color="#31849b"
     protein_bind    2933..2954
                     /label="CAP binding site"
                     /color="#31849b"
     primer_bind     223..239
                     /label="M13 rev"
                     /color="#a020f0"
     primer_bind     309..325
                     /label="M13 fwd"
                     /color="#a020f0"
ORIGIN      
        1 TTAATTAAGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA
       61 TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG
      121 CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG
      181 CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC AGCTATGACC
      241 ATGATTACGC CAAGCTTGCA TGCCTGCAGG TCGACTCTAG AGGATCCCCG GGTACCGAGC
      301 TCGAATTCAC TGGCCGTCGT TTTACAACGT CGTGACTGGG AAAACCCTGG CGTTACCCAA
      361 CTTAATCGCC TTGCAGCACA TCCCCCTTTC GCCAGCTGGC GTAATAGCGA AGAGGCCCGC
      421 ACCGATCGCC CTTCCCAACA GTTGCGCAGC CTGAATGGCG AATGGCGCCT GATGCGGTAT
      481 TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT GGTGCACTCT CAGTACAATC
      541 TGCTCTGATG CCGCATAGTT AAGCCAGCCC CGACACCCGC CAACACCCGC TGAACTAGTC
      601 TTGGACTCCT GTTGATAGAT CCAGTAATGA CCTCAGAACT CCATCTGGAT TTGTTCAGAA
      661 CGCTCGGTTG CCGCCGGGCG TTTTTTATTG GTGAGAATCC AGGGGTCCCC AATAATTACG
      721 ATTTAAATTA GTAGCCCGCC TAATGAGCGG GCTTTTTTTT AATTCCCCTA TTTGTTTATT
      781 TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA
      841 ATAATATTGA AAAAGGAAGA GTATGAGCAT TCAGCATTTT CGTGTGGCGC TGATTCCGTT
      901 TTTTGCGGCG TTTTGCCTGC CGGTGTTTGC GCATCCGGAA ACCCTGGTGA AAGTGAAAGA
      961 TGCGGAAGAT CAACTGGGTG CGCGCGTGGG CTATATTGAA CTGGATCTGA ACAGCGGCAA
     1021 AATTCTGGAA TCTTTTCGTC CGGAAGAACG TTTTCCGATG ATGAGCACCT TTAAAGTGCT
     1081 GCTGTGCGGT GCGGTTCTGA GCCGTGTGGA TGCGGGCCAG GAACAACTGG GCCGTCGTAT
     1141 TCATTATAGC CAGAACGATC TGGTGGAATA TAGCCCGGTG ACCGAAAAAC ATCTGACCGA
     1201 TGGCATGACC GTGCGTGAAC TGTGCAGCGC GGCGATTACC ATGAGCGATA ACACCGCGGC
     1261 GAACCTGCTG CTGACGACCA TTGGCGGTCC GAAAGAACTG ACCGCGTTTC TGCATAACAT
     1321 GGGCGATCAT GTGACCCGTC TGGATCGTTG GGAACCGGAA CTGAACGAAG CGATTCCGAA
     1381 CGATGAACGT GATACCACCA TGCCGGCAGC AATGGCGACC ACCCTGCGTA AACTGCTGAC
     1441 GGGTGAGCTG CTGACCCTGG CAAGCCGCCA GCAACTGATT GATTGGATGG AAGCGGATAA
     1501 AGTGGCGGGT CCGCTGCTGC GTAGCGCGCT GCCGGCTGGC TGGTTTATTG CGGATAAAAG
     1561 CGGTGCGGGC GAACGTGGCA GCCGTGGCAT TATTGCGGCG CTGGGCCCGG ATGGTAAACC
     1621 GAGCCGTATT GTGGTGATTT ATACCACCGG CAGCCAGGCG ACGATGGATG AACGTAACCG
     1681 TCAGATTGCG GAAATTGGCG CGAGCCTGAT TAAACATTGG TAAACCGATA CAATTAAAGG
     1741 CTCCTTTTGG AGCCTTTTTT TTTGGACGAC CCTTGTCCTT TTCCGCTGCA TAACCCTGCT
     1801 TCGGGGTCAT TATAGCGATT TTTTCGGTAT ATCCATCCTT TTTCGCACGA TATACAGGAT
     1861 TTTGCCAAAG GGTTCGTGTA GACTTTCCTT GGTGTATCCA ACGGCGTCAG CCGGGCAGGA
     1921 TAGGTGAAGT AGGCCCACCC GCGAGCGGGT GTTCCTTCTT CACTGTCCCT TATTCGCACC
     1981 TGGCGGTGCT CAACGGGAAT CCTGCTCTGC GAGGCTGGCC GTAGGCCGGC CCCGTAGAAA
     2041 AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA
     2101 AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC
     2161 CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT
     2221 AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC
     2281 TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC
     2341 GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA
     2401 GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTT TGAGAAAGCG
     2461 CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG
     2521 GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT
     2581 TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT
     2641 GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC
     2701 ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT
     2761 GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG
     2821 CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA
     2881 GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA
     2941 GTTAGCTCAC TCATTAGGCA GGCGCGCCCA GCTGTCTAGG GCGGCGGATT TGTCCTACTC
     3001 AGGAGAGCGT TCACCGACAA ACAACAGATA AAACGAAAGG CCCAGTCTTT CGACTGAGCC
     3061 TTTCGTTTTA TTTGATGCCT    
//
