LOCUS       Untitled_Sequence        3615 bp    DNA     circular SYN 28-FEB-2023
FEATURES             Location/Qualifiers
     rep_origin      2639..3410
                     /label="pBBR1 oriV"
                     /color="#ffff00"
     CDS             1976..2638
                     /label="pBBR1 Rep"
                     /color="#993366"
     CDS             1113..1646
                     /label="GmR"
                     /color="#ccffcc"
     CDS             255..578
                     /label="lacZα"
                     /color="#993366"
     oriT            1857..1966
                     /label="oriT"
                     /color="#a6acb3"
     terminator      771..865
                     /label="lambda t0 terminator"
                     /color="#ffffff"
     terminator      3525..3611
                     /label="rrnB T1 terminator"
                     /color="#ffffff"
     misc_feature    272..328
                     /label="MCS"
                     /color="#99ccff"
     promoter        205..211
                     /label="lac promoter"
                     /color="#ffffff"
     promoter        919..924
                     /label="Pc promoter"
                     /color="#ffffff"
     protein_bind    145..166
                     /label="CAP binding site"
                     /color="#31849b"
     protein_bind    219..235
                     /label="lac operator"
                     /color="#31849b"
     primer_bind     243..259
                     /label="M13 rev"
                     /color="#a020f0"
     primer_bind     329..345
                     /label="M13 fwd"
                     /color="#a020f0"
     misc_feature    87..90
                     /label="1"
                     /color="#99cc00"
     misc_feature    83..86
                     /label="B"
                     /color="#00ffff"
     misc_feature    675..678
                     /label="4"
                     /color="#99cc00"
     misc_feature    679..682
                     /label="C"
                     /color="#00ffff"
ORIGIN      
        1 TTAATTAAAG CGGATAACAA TTTCACACAG GAGGCCGCCT AGGCCGCGGC CGCGCGAATT
       61 CGAGCTCGGT ACCCGGGTCT CATACTTGCC TTGTCTTCAC GACAGGTTTG CCGACTGGAA
      121 AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC
      181 TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA
      241 CACAGGAAAC AGCTATGACC ATGATTACGC CAAGCTTGCA TGCCTGCAGG TCGACTCTAG
      301 AGGATCCCCG GGTACCGAGC TCGAATTCAC TGGCCGTCGT TTTACAACGT CGTGACTGGG
      361 AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA TCCCCCTTTC GCCAGCTGGC
      421 GTAATAGCGA AGAGGCCCGC ACCGATCGCC CTTCCCAACA GTTGCGCAGC CTGAATGGCG
      481 AATGGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT
      541 GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGCCC CGACACCCGC
      601 CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG
      661 CTGTGAGAAG ACAATTACCC ATTGAGACCC GGGGATCCTC TAGAGTCGAC CTGCAGGCAT
      721 GCAAGCTTGC GGCCGCGTCG TGACTGGGAA AACCCTGGCG ACTAGTCTTG GACTCCTGTT
      781 GATAGATCCA GTAATGACCT CAGAACTCCA TCTGGATTTG TTCAGAACGC TCGGTTGCCG
      841 CCGGGCGTTT TTTATTGGTG AGAATCCAGG GGTCCCCAAT AATTACGATT TAAATTTGAC
      901 ATAAGCCTGT TCGGTTCGTA AACTGTAATG CAAGTAGCGT ATGCGCTCAC GCAACTGGTC
      961 CAGAACCTTG ACCGAACGCA GCGGTGGTAA CGGCGCAGTG GCGGTTTTCA TGGCTTGTTA
     1021 TGACTGTTTT TTTGTACAGC CTATGCCTCG GGCATCCAAG CAGCAAGCGC GTTACGCCGT
     1081 GGGTCGATGT TTGATGTTAT GGAGCAGCAA CGATGTTACG CAGCAGCAAC GATGTTACGC
     1141 AGCAGGGCAG TCGCCCTAAA ACAAAGTTAG GTGGCTCAAG TATGGGCATC ATTCGCACAT
     1201 GTAGGCTCGG CCCTGACCAA GTCAAATCCA TGCGGGCTGC TCTTGATCTT TTCGGTCGTG
     1261 AGTTCGGAGA CGTAGCCACC TACTCCCAAC ATCAGCCGGA CTCCGATTAC CTCGGGAACT
     1321 TGCTCCGTAG TAAGACATTC ATCGCGCTTG CTGCCTTCGA CCAAGAAGCG GTTGTTGGCG
     1381 CTCTCGCGGC TTACGTTCTG CCCAAGTTTG AGCAGCCGCG TAGTGAGATC TATATCTATG
     1441 ATCTCGCAGT CTCCGGAGAG CACCGGAGGC AGGGCATTGC CACCGCGCTC ATCAATCTCC
     1501 TCAAGCATGA GGCCAACGCG CTTGGTGCTT ATGTGATCTA CGTGCAAGCA GATTACGGTG
     1561 ACGATCCCGC AGTGGCTCTC TATACAAAGT TGGGCATACG GGAAGAAGTG ATGCACTTTG
     1621 ATATCGACCC AAGTACCGCC ACCTAACAAT TCGTTCAAGC CGAGATCGGC TTCCCGGCCG
     1681 CGGAGTTGTT CGGTAAATTG GACAACGGTC CGCGCGTTGT CCTTTTCCGC TGCATAACCC
     1741 TGCTTCGGGG TCATTATAGC GATTTTTTCG GTATATCCAT CCTTTTTCGC ACGATATACA
     1801 GGATTTTGCC AAAGGGTTCG TGTAGACTTT CCTTGGTGTA TCCAACGGCG TCAGCCGGGC
     1861 AGGATAGGTG AAGTAGGCCC ACCCGCGAGC GGGTGTTCCT TCTTCACTGT CCCTTATTCG
     1921 CACCTGGCGG TGCTCAACGG GAATCCTGCT CTGCGAGGCT GGCCGTAGGC CGGCCCTACC
     1981 GGCGCGGCAG CGTTACCCGT GTCGGCGGCT CCAACGGCTC GCCATCGTCC AGAAAACACG
     2041 GCTCATCGGG CATCGGCAGG CGCTGCTGCC CGCGCCGTTC CCATTCCTCC GTTTCGGTCA
     2101 AGGCTGGCAG GTCTGGTTCC ATGCCCGGAA TGCCGGGCTG GCTGGGCGGC TCCTCGCCGG
     2161 GGCCGGTCGG TAGTTGCTGC TCGCCCGGAT ACAGGGTCGG GATGCGGCGC AGGTCGCCAT
     2221 GCCCCAACAG CGATTCGTCC TGGTCGTCGT GATCAACCAC CACGGCGGCA CTGAACACCG
     2281 ACAGGCGCAA CTGGTCGCGG GGCTGGCCCC ACGCCACGCG GTCATTGACC ACGTAGGCCG
     2341 ACACGGTGCC GGGGCCGTTG AGCTTCACGA CGGAGATCCA GCGCTCGGCC ACCAAGTCCT
     2401 TGACTGCGTA TTGGACCGTC CGCAAAGAAC GTCCGATGAG CTTGGAAAGT GTTTTCTGGC
     2461 TGACCACCAC GGCGTTCTGG TGGCCCATCT GCGCCACGAG GTGATGCAGC AGCATTGCCG
     2521 CCGTGGGTTT CCTCGCAATA AGCCCGGCCC ACGCCTCATG CGCTTTGCGT TCCGTTTGCA
     2581 CCCAGTGACC GGGCTTGTTC TTGGCTTGAA TGCCGATTTC TCTGGACTGC GTGGCCATGC
     2641 TTATCTCCAT GCGGTAGGGG TGCCGCACGG TTGCGGCACC ATGCGCAATC AGCTGCAACT
     2701 TTTCGGCAGC GCGACAACAA TTATGCGTTG CGTAAAAGTG GCAGTCAATT ACAGATTTTC
     2761 TTTAACCTAC GCAATGAGCT ATTGCGGGGG GTGCCGCAAT GAGCTGTTGC GTACCCCCCT
     2821 TTTTTAAGTT GTTGATTTTT AAGTCTTTCG CATTTCGCCC TATATCTAGT TCTTTGGTGC
     2881 CCAAAGAAGG GCACCCCTGC GGGGTTCCCC CACGCCTTCG GCGCGGCTCC CCCTCCGGCA
     2941 AAAAGTGGCC CCTCCGGGGC TTGTTGATCG ACTGCGCGGC CTTCGGCCTT GCCCAAGGTG
     3001 GCGCTGCCCC CTTGGAACCC CCGCACTCGC CGCCGTGAGG CTCGGGGGGC AGGCGGGCGG
     3061 GCTTCGCCCT TCGACTGCCC CCACTCGCAT AGGCTTGGGT CGTTCCAGGC GCGTCAAGGC
     3121 CAAGCCGCTG CGCGGTCGCT GCGCGAGCCT TGACCCGCCT TCCACTTGGT GTCCAACCGG
     3181 CAAGCGAAGC GCGCAGGCCG CAGGCCGGAG GCTTTTCCCC AGAGAAAATT AAAAAAATTG
     3241 ATGGGGCAAG GCCGCAGGCC GCGCAGTTGG AGCCGGTGGG TATGTGGTCG AAGGCTGGGT
     3301 AGCCGGTGGG CAATCCCTGT GGTCAAGCTC GTGGGCAGGC GCAGCCTGTC CATCAGCTTG
     3361 TCCAGCAGGG TTGTCCACGG GCCGAGCGAA GCGAGCCAGC CGGTGGCCGC TCGCGGCCAT
     3421 CGTCCACATA TCCACGGGCT GGCAAGGGAG CGCAGCGACC GCGCAGGGCG AAGCCCGGAG
     3481 AGCAAGCCCG TAGGGGGGGC GCGCCCAGCT GTCTAGGGCG GCGGATTTGT CCTACTCAGG
     3541 AGAGCGTTCA CCGACAAACA ACAGATAAAA CGAAAGGCCC AGTCTTTCGA CTGAGCCTTT
     3601 CGTTTTATTT GATGC    
//