LOCUS       Untitled_Sequence        3614 bp    DNA     circular SYN 28-FEB-2023
FEATURES             Location/Qualifiers
     primer_bind     329..345
                     /label="M13 fwd"
                     /color="#a020f0"
     primer_bind     243..259
                     /label="M13 rev"
                     /color="#a020f0"
     protein_bind    219..235
                     /label="lac operator"
                     /color="#31849b"
     protein_bind    145..166
                     /label="CAP binding site"
                     /color="#31849b"
     promoter        918..923
                     /label="Pc promoter"
                     /color="#ffffff"
     promoter        205..211
                     /label="lac promoter"
                     /color="#ffffff"
     misc_feature    272..328
                     /label="MCS"
                     /color="#99ccff"
     terminator      3524..3610
                     /label="rrnB T1 terminator"
                     /color="#ffffff"
     terminator      770..864
                     /label="lambda t0 terminator"
                     /color="#ffffff"
     oriT            1856..1964
                     /label="oriT"
                     /color="#a6acb3"
     CDS             255..578
                     /label="lacZα"
                     /color="#993366"
     CDS             1112..1645
                     /label="GmR"
                     /color="#ccffcc"
     CDS             1975..2637
                     /label="pBBR1 Rep"
                     /color="#993366"
     rep_origin      2638..3409
                     /label="pBBR1 oriV"
                     /color="#ffff00"
     misc_feature    87..90
                     /label="1"
                     /color="#99cc00"
     misc_feature    83..86
                     /label="C"
                     /color="#00ffff"
     misc_feature    675..678
                     /label="5"
                     /color="#99cc00"
     misc_feature    679..682
                     /label="D"
                     /color="#00ffff"
ORIGIN      
        1 TTAATTAAAG CGGATAACAA TTTCACACAG GAGGCCGCCT AGGCCGCGGC CGCGCGAATT
       61 CGAGCTCGGT ACCCGGGTCT CACCATTGCC TTGTCTTCAC GACAGGTTTG CCGACTGGAA
      121 AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC
      181 TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA
      241 CACAGGAAAC AGCTATGACC ATGATTACGC CAAGCTTGCA TGCCTGCAGG TCGACTCTAG
      301 AGGATCCCCG GGTACCGAGC TCGAATTCAC TGGCCGTCGT TTTACAACGT CGTGACTGGG
      361 AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA TCCCCCTTTC GCCAGCTGGC
      421 GTAATAGCGA AGAGGCCCGC ACCGATCGCC CTTCCCAACA GTTGCGCAGC CTGAATGGCG
      481 AATGGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT
      541 GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGCCC CGACACCCGC
      601 CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG
      661 CTGTGAGAAG ACAACAGAAA TGTGAGACCG GGGATCCTCT AGAGTCGACC TGCAGGCATG
      721 CAAGCTTGCG GCCGCGTCGT GACTGGGAAA ACCCTGGCGA CTAGTCTTGG ACTCCTGTTG
      781 ATAGATCCAG TAATGACCTC AGAACTCCAT CTGGATTTGT TCAGAACGCT CGGTTGCCGC
      841 CGGGCGTTTT TTATTGGTGA GAATCCAGGG GTCCCCAATA ATTACGATTT AAATTTGACA
      901 TAAGCCTGTT CGGTTCGTAA ACTGTAATGC AAGTAGCGTA TGCGCTCACG CAACTGGTCC
      961 AGAACCTTGA CCGAACGCAG CGGTGGTAAC GGCGCAGTGG CGGTTTTCAT GGCTTGTTAT
     1021 GACTGTTTTT TTGTACAGCC TATGCCTCGG GCATCCAAGC AGCAAGCGCG TTACGCCGTG
     1081 GGTCGATGTT TGATGTTATG GAGCAGCAAC GATGTTACGC AGCAGCAACG ATGTTACGCA
     1141 GCAGGGCAGT CGCCCTAAAA CAAAGTTAGG TGGCTCAAGT ATGGGCATCA TTCGCACATG
     1201 TAGGCTCGGC CCTGACCAAG TCAAATCCAT GCGGGCTGCT CTTGATCTTT TCGGTCGTGA
     1261 GTTCGGAGAC GTAGCCACCT ACTCCCAACA TCAGCCGGAC TCCGATTACC TCGGGAACTT
     1321 GCTCCGTAGT AAGACATTCA TCGCGCTTGC TGCCTTCGAC CAAGAAGCGG TTGTTGGCGC
     1381 TCTCGCGGCT TACGTTCTGC CCAAGTTTGA GCAGCCGCGT AGTGAGATCT ATATCTATGA
     1441 TCTCGCAGTC TCCGGAGAGC ACCGGAGGCA GGGCATTGCC ACCGCGCTCA TCAATCTCCT
     1501 CAAGCATGAG GCCAACGCGC TTGGTGCTTA TGTGATCTAC GTGCAAGCAG ATTACGGTGA
     1561 CGATCCCGCA GTGGCTCTCT ATACAAAGTT GGGCATACGG GAAGAAGTGA TGCACTTTGA
     1621 TATCGACCCA AGTACCGCCA CCTAACAATT CGTTCAAGCC GAGATCGGCT TCCCGGCCGC
     1681 GGAGTTGTTC GGTAAATTGG ACAACGGTCC GCGCGTTGTC CTTTTCCGCT GCATAACCCT
     1741 GCTTCGGGGT CATTATAGCG ATTTTTTCGG TATATCCATC CTTTTTCGCA CGATATACAG
     1801 GATTTTGCCA AAGGGTTCGT GTAGACTTTC CTTGGTGTAT CCAACGGCGT CAGCCGGGCA
     1861 GGATAGGTGA AGTAGGCCCA CCCGCGAGCG GGTGTTCCTT CTTCACTGTC CCTTATTCGC
     1921 ACCTGGCGGT GCTCAACGGG AATCCTGCTC TGCGAGGCTG GCCGTAGGCC GGCCCTACCG
     1981 GCGCGGCAGC GTTACCCGTG TCGGCGGCTC CAACGGCTCG CCATCGTCCA GAAAACACGG
     2041 CTCATCGGGC ATCGGCAGGC GCTGCTGCCC GCGCCGTTCC CATTCCTCCG TTTCGGTCAA
     2101 GGCTGGCAGG TCTGGTTCCA TGCCCGGAAT GCCGGGCTGG CTGGGCGGCT CCTCGCCGGG
     2161 GCCGGTCGGT AGTTGCTGCT CGCCCGGATA CAGGGTCGGG ATGCGGCGCA GGTCGCCATG
     2221 CCCCAACAGC GATTCGTCCT GGTCGTCGTG ATCAACCACC ACGGCGGCAC TGAACACCGA
     2281 CAGGCGCAAC TGGTCGCGGG GCTGGCCCCA CGCCACGCGG TCATTGACCA CGTAGGCCGA
     2341 CACGGTGCCG GGGCCGTTGA GCTTCACGAC GGAGATCCAG CGCTCGGCCA CCAAGTCCTT
     2401 GACTGCGTAT TGGACCGTCC GCAAAGAACG TCCGATGAGC TTGGAAAGTG TTTTCTGGCT
     2461 GACCACCACG GCGTTCTGGT GGCCCATCTG CGCCACGAGG TGATGCAGCA GCATTGCCGC
     2521 CGTGGGTTTC CTCGCAATAA GCCCGGCCCA CGCCTCATGC GCTTTGCGTT CCGTTTGCAC
     2581 CCAGTGACCG GGCTTGTTCT TGGCTTGAAT GCCGATTTCT CTGGACTGCG TGGCCATGCT
     2641 TATCTCCATG CGGTAGGGGT GCCGCACGGT TGCGGCACCA TGCGCAATCA GCTGCAACTT
     2701 TTCGGCAGCG CGACAACAAT TATGCGTTGC GTAAAAGTGG CAGTCAATTA CAGATTTTCT
     2761 TTAACCTACG CAATGAGCTA TTGCGGGGGG TGCCGCAATG AGCTGTTGCG TACCCCCCTT
     2821 TTTTAAGTTG TTGATTTTTA AGTCTTTCGC ATTTCGCCCT ATATCTAGTT CTTTGGTGCC
     2881 CAAAGAAGGG CACCCCTGCG GGGTTCCCCC ACGCCTTCGG CGCGGCTCCC CCTCCGGCAA
     2941 AAAGTGGCCC CTCCGGGGCT TGTTGATCGA CTGCGCGGCC TTCGGCCTTG CCCAAGGTGG
     3001 CGCTGCCCCC TTGGAACCCC CGCACTCGCC GCCGTGAGGC TCGGGGGGCA GGCGGGCGGG
     3061 CTTCGCCCTT CGACTGCCCC CACTCGCATA GGCTTGGGTC GTTCCAGGCG CGTCAAGGCC
     3121 AAGCCGCTGC GCGGTCGCTG CGCGAGCCTT GACCCGCCTT CCACTTGGTG TCCAACCGGC
     3181 AAGCGAAGCG CGCAGGCCGC AGGCCGGAGG CTTTTCCCCA GAGAAAATTA AAAAAATTGA
     3241 TGGGGCAAGG CCGCAGGCCG CGCAGTTGGA GCCGGTGGGT ATGTGGTCGA AGGCTGGGTA
     3301 GCCGGTGGGC AATCCCTGTG GTCAAGCTCG TGGGCAGGCG CAGCCTGTCC ATCAGCTTGT
     3361 CCAGCAGGGT TGTCCACGGG CCGAGCGAAG CGAGCCAGCC GGTGGCCGCT CGCGGCCATC
     3421 GTCCACATAT CCACGGGCTG GCAAGGGAGC GCAGCGACCG CGCAGGGCGA AGCCCGGAGA
     3481 GCAAGCCCGT AGGGGGGGCG CGCCCAGCTG TCTAGGGCGG CGGATTTGTC CTACTCAGGA
     3541 GAGCGTTCAC CGACAAACAA CAGATAAAAC GAAAGGCCCA GTCTTTCGAC TGAGCCTTTC
     3601 GTTTTATTTG ATGC    
//