LOCUS       pSEVA63g19[gD]          3616 bp ds-DNA     circular     22-SEP-2022
DEFINITION  .
KEYWORDS    "creator:Centro Nacional De Biotecnologia" "marker:GmR"
FEATURES             Location/Qualifiers
     terminator      22..108
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="gene: Escherichia coli rrnB
note: transcription terminator T1 from the E. coli rrnB gene"
     primer          190..232
                     /label="Primer D1"
                     /note="sequence: GGTCTCaAATGTGCCttGTCTTCacgacaggtttgccgactgg"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    197..200
                     /label="D"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    201..204
                     /label=1
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     protein_bind    259..280
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     promoter        295..300
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        301..318
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        319..325
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind    333..349
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     primer_bind     357..373
                     /label="M13 rev"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
     CDS             369..692
                     /label="lacZα"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     misc_feature    386..442
                     /label="MCS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     complement(443..459)
                     /label="M13 fwd"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
     primer          complement(756..803)
                     /label="Primer 6E"
                     /note="sequence: GGTCTCaACCTCACAttGTCTTCtcacagcttgtctgtaagcggatgc"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    789..792
                     /label=6
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    793..796
                     /label="E"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     terminator      884..978
                     /label="lambda t0 terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="gene: 
note: transcription terminator from phage lambda"
     promoter        1009..1014
                     /label="Pc promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="gene: intI1 (promoter lies within the coding sequence)
note: class 1 integron promoter"
     promoter        1015..1031
                     /label="Pc promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="gene: intI1 (promoter lies within the coding sequence)
note: class 1 integron promoter"
     promoter        1032..1037
                     /label="Pc promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="gene: intI1 (promoter lies within the coding sequence)
note: class 1 integron promoter"
     CDS             1226..1759
                     /label="GmR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="allele: 
codon_start: 1
gene: aacC1
note: confers resistance to gentamycin
product: gentamycin acetyltransferase
transl_table: 1
translation: MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALDLFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRSEIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIREEVMHFDIDPSTAT*"
     oriT            1970..2078
                     /label="oriT"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: incP origin of transfer"
     CDS             complement(2089..2751)
                     /label="pBBR1 Rep"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     misc_feature    2089..3610
                     /label="pBBR1* Ori"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     rep_origin      2752..3523
                     /label="pBBR1 oriV"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 CCCAGCTGTC TAGGGCGGCG GATTTGTCCT ACTCAGGAGA GCGTTCACCG ACAAACAACA
       61 GATAAAACGA AAGGCCCAGT CTTTCGACTG AGCCTTTCGT TTTATTTGAT GCCTTTAATT
      121 AAAGCGGATA ACAATTTCAC ACAGGAGGCC GCCTAGGCCG CGGCCGCGCG AATTCGAGCT
      181 CGGTACCCGG GTCTCAAATG TGCCTTGTCT TCACGACAGG TTTGCCGACT GGAAAGCGGG
      241 CAGTGAGCGC AACGCAATTA ATGTGAGTTA GCTCACTCAT TAGGCACCCC AGGCTTTACA
      301 CTTTATGCTT CCGGCTCGTA TGTTGTGTGG AATTGTGAGC GGATAACAAT TTCACACAGG
      361 AAACAGCTAT GACCATGATT ACGCCAAGCT TGCATGCCTG CAGGTCGACT CTAGAGGATC
      421 CCCGGGTACC GAGCTCGAAT TCACTGGCCG TCGTTTTACA ACGTCGTGAC TGGGAAAACC
      481 CTGGCGTTAC CCAACTTAAT CGCCTTGCAG CACATCCCCC TTTCGCCAGC TGGCGTAATA
      541 GCGAAGAGGC CCGCACCGAT CGCCCTTCCC AACAGTTGCG CAGCCTGAAT GGCGAATGGC
      601 GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATGGTGCA
      661 CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GCCCCGACAC CCGCCAACAC
      721 CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA
      781 GAAGACAATG TGAGGTTGAG ACCGGGGATC CTCTAGAGTC GACCTGCAGG CATGCAAGCT
      841 TGCGGCCGCG TCGTGACTGG GAAAACCCTG GCGACTAGTC TTGGACTCCT GTTGATAGAT
      901 CCAGTAATGA CCTCAGAACT CCATCTGGAT TTGTTCAGAA CGCTCGGTTG CCGCCGGGCG
      961 TTTTTTATTG GTGAGAATCC AGGGGTCCCC AATAATTACG ATTTAAATTT GACATAAGCC
     1021 TGTTCGGTTC GTAAACTGTA ATGCAAGTAG CGTATGCGCT CACGCAACTG GTCCAGAACC
     1081 TTGACCGAAC GCAGCGGTGG TAACGGCGCA GTGGCGGTTT TCATGGCTTG TTATGACTGT
     1141 TTTTTTGTAC AGCCTATGCC TCGGGCATCC AAGCAGCAAG CGCGTTACGC CGTGGGTCGA
     1201 TGTTTGATGT TATGGAGCAG CAACGATGTT ACGCAGCAGC AACGATGTTA CGCAGCAGGG
     1261 CAGTCGCCCT AAAACAAAGT TAGGTGGCTC AAGTATGGGC ATCATTCGCA CATGTAGGCT
     1321 CGGCCCTGAC CAAGTCAAAT CCATGCGGGC TGCTCTTGAT CTTTTCGGTC GTGAGTTCGG
     1381 AGACGTAGCC ACCTACTCCC AACATCAGCC GGACTCCGAT TACCTCGGGA ACTTGCTCCG
     1441 TAGTAAGACA TTCATCGCGC TTGCTGCCTT CGACCAAGAA GCGGTTGTTG GCGCTCTCGC
     1501 GGCTTACGTT CTGCCCAAGT TTGAGCAGCC GCGTAGTGAG ATCTATATCT ATGATCTCGC
     1561 AGTCTCCGGA GAGCACCGGA GGCAGGGCAT TGCCACCGCG CTCATCAATC TCCTCAAGCA
     1621 TGAGGCCAAC GCGCTTGGTG CTTATGTGAT CTACGTGCAA GCAGATTACG GTGACGATCC
     1681 CGCAGTGGCT CTCTATACAA AGTTGGGCAT ACGGGAAGAA GTGATGCACT TTGATATCGA
     1741 CCCAAGTACC GCCACCTAAC AATTCGTTCA AGCCGAGATC GGCTTCCCGG CCGCGGAGTT
     1801 GTTCGGTAAA TTGGACAACG GTCCGCGCGT TGTCCTTTTC CGCTGCATAA CCCTGCTTCG
     1861 GGGTCATTAT AGCGATTTTT TCGGTATATC CATCCTTTTT CGCACGATAT ACAGGATTTT
     1921 GCCAAAGGGT TCGTGTAGAC TTTCCTTGGT GTATCCAACG GCGTCAGCCG GGCAGGATAG
     1981 GTGAAGTAGG CCCACCCGCG AGCGGGTGTT CCTTCTTCAC TGTCCCTTAT TCGCACCTGG
     2041 CGGTGCTCAA CGGGAATCCT GCTCTGCGAG GCTGGCCGTA GGCCGGCCCT ACCGGCGCGG
     2101 CAGCGTTACC CGTGTCGGCG GCTCCAACGG CTCGCCATCG TCCAGAAAAC ACGGCTCATC
     2161 GGGCATCGGC AGGCGCTGCT GCCCGCGCCG TTCCCATTCC TCCGTTTCGG TCAAGGCTGG
     2221 CAGGTCTGGT TCCATGCCCG GAATGCCGGG CTGGCTGGGC GGCTCCTCGC CGGGGCCGGT
     2281 CGGTAGTTGC TGCTCGCCCG GATACAGGGT CGGGATGCGG CGCAGGTCGC CATGCCCCAA
     2341 CAGCGATTCG TCCTGGTCGT CGTGATCAAC CACCACGGCG GCACTGAACA CCGACAGGCG
     2401 CAACTGGTCG CGGGGCTGGC CCCACGCCAC GCGGTCATTG ACCACGTAGG CCGACACGGT
     2461 GCCGGGGCCG TTGAGCTTCA CGACGGAGAT CCAGCGCTCG GCCACCAAGT CCTTGACTGC
     2521 GTATTGGACC GTCCGCAAAG AACGTCCGAT GAGCTTGGAA AGTGTTTTCT GGCTGACCAC
     2581 CACGGCGTTC TGGTGGCCCA TCTGCGCCAC GAGGTGATGC AGCAGCATTG CCGCCGTGGG
     2641 TTTCCTCGCA ATAAGCCCGG CCCACGCCTC ATGCGCTTTG CGTTCCGTTT GCACCCAGTG
     2701 ACCGGGCTTG TTCTTGGCTT GAATGCCGAT TTCTCTGGAC TGCGTGGCCA TGCTTATCTC
     2761 CATGCGGTAG GGGTGCCGCA CGGTTGCGGC ACCATGCGCA ATCAGCTGCA ACTTTTCGGC
     2821 AGCGCGACAA CAATTATGCG TTGCGTAAAA GTGGCAGTCA ATTACAGATT TTCTTTAACC
     2881 TACGCAATGA GCTATTGCGG GGGGTGCCGC AATGAGCTGT TGCGTACCCC CCTTTTTTAA
     2941 GTTGTTGATT TTTAAGTCTT TCGCATTTCG CCCTATATCT AGTTCTTTGG TGCCCAAAGA
     3001 AGGGCACCCC TGCGGGGTTC CCCCACGCCT TCGGCGCGGC TCCCCCTCCG GCAAAAAGTG
     3061 GCCCCTCCGG GGCTTGTTGA TCGACTGCGC GGCCTTCGGC CTTGCCCAAG GTGGCGCTGC
     3121 CCCCTTGGAA CCCCCGCACT CGCCGCCGTG AGGCTCGGGG GGCAGGCGGG CGGGCTTCGC
     3181 CCTTCGACTG CCCCCACTCG CATAGGCTTG GGTCGTTCCA GGCGCGTCAA GGCCAAGCCG
     3241 CTGCGCGGTC GCTGCGCGAG CCTTGACCCG CCTTCCACTT GGTGTCCAAC CGGCAAGCGA
     3301 AGCGCGCAGG CCGCAGGCCG GAGGCTTTTC CCCAGAGAAA ATTAAAAAAA TTGATGGGGC
     3361 AAGGCCGCAG GCCGCGCAGT TGGAGCCGGT GGGTATGTGG TCGAAGGCTG GGTAGCCGGT
     3421 GGGCAATCCC TGTGGTCAAG CTCGTGGGCA GGCGCAGCCT GTCCATCAGC TTGTCCAGCA
     3481 GGGTTGTCCA CGGGCCGAGC GAAGCGAGCC AGCCGGTGGC CGCTCGCGGC CATCGTCCAC
     3541 ATATCCACGG GCTGGCAAGG GAGCGCAGCG ACCGCGCAGG GCGAAGCCCG GAGAGCAAGC
     3601 CCGTAGGGGG GGCGCG
//
