LOCUS       Untitled_Sequence        3613 bp    DNA     circular SYN 28-FEB-2023
DEFINITION  <div>CMC-MoCLo vector CMCv11</div><div>for MoClo Level 2 assembly</div><div>E17F</div>


FEATURES             Location/Qualifiers
     rep_origin      2637..3408
                     /label="pBBR1 oriV"
                     /color="#ffff00"
     CDS             1974..2636
                     /label="pBBR1 Rep"
                     /color="#993366"
     CDS             1111..1644
                     /label="GmR"
                     /color="#ccffcc"
     CDS             254..577
                     /label="lacZα"
                     /color="#993366"
     oriT            1855..1964
                     /label="oriT"
                     /color="#a6acb3"
     terminator      769..863
                     /label="lambda t0 terminator"
                     /color="#ffffff"
     terminator      3523..3609
                     /label="rrnB T1 terminator"
                     /color="#ffffff"
     misc_feature    271..327
                     /label="MCS"
                     /color="#99ccff"
     promoter        204..210
                     /label="lac promoter"
                     /color="#ffffff"
     promoter        917..922
                     /label="Pc promoter"
                     /color="#ffffff"
     protein_bind    144..165
                     /label="CAP binding site"
                     /color="#31849b"
     protein_bind    218..234
                     /label="lac operator"
                     /color="#31849b"
     primer_bind     242..258
                     /label="M13 rev"
                     /color="#a020f0"
     primer_bind     328..344
                     /label="M13 fwd"
                     /color="#a020f0"
     misc_feature    86..89
                     /label="1"
                     /color="#99cc00"
     misc_feature    674..677
                     /label="7"
                     /color="#99cc00"
     misc_feature    82..85
                     /label="E"
                     /color="#00ffff"
     misc_feature    678..681
                     /label="F"
                     /color="#00ffff"
ORIGIN      
        1 TTAATTAAAG CGGATAACAA TTTCACACAG GAGGCCGCCT AGGCCGCGGC CGCGCGAATT
       61 CGAGCTCGGT ACCCGGTCTC AAGGTTGCCT TGTCTTCACG ACAGGTTTGC CGACTGGAAA
      121 GCGGGCAGTG AGCGCAACGC AATTAATGTG AGTTAGCTCA CTCATTAGGC ACCCCAGGCT
      181 TTACACTTTA TGCTTCCGGC TCGTATGTTG TGTGGAATTG TGAGCGGATA ACAATTTCAC
      241 ACAGGAAACA GCTATGACCA TGATTACGCC AAGCTTGCAT GCCTGCAGGT CGACTCTAGA
      301 GGATCCCCGG GTACCGAGCT CGAATTCACT GGCCGTCGTT TTACAACGTC GTGACTGGGA
      361 AAACCCTGGC GTTACCCAAC TTAATCGCCT TGCAGCACAT CCCCCTTTCG CCAGCTGGCG
      421 TAATAGCGAA GAGGCCCGCA CCGATCGCCC TTCCCAACAG TTGCGCAGCC TGAATGGCGA
      481 ATGGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATG
      541 GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGCCCC GACACCCGCC
      601 AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC
      661 TGTGAGAAGA CAAGAGCTTC GTGAGACCGG GGATCCTCTA GAGTCGACCT GCAGGCATGC
      721 AAGCTTGCGG CCGCGTCGTG ACTGGGAAAA CCCTGGCGAC TAGTCTTGGA CTCCTGTTGA
      781 TAGATCCAGT AATGACCTCA GAACTCCATC TGGATTTGTT CAGAACGCTC GGTTGCCGCC
      841 GGGCGTTTTT TATTGGTGAG AATCCAGGGG TCCCCAATAA TTACGATTTA AATTTGACAT
      901 AAGCCTGTTC GGTTCGTAAA CTGTAATGCA AGTAGCGTAT GCGCTCACGC AACTGGTCCA
      961 GAACCTTGAC CGAACGCAGC GGTGGTAACG GCGCAGTGGC GGTTTTCATG GCTTGTTATG
     1021 ACTGTTTTTT TGTACAGCCT ATGCCTCGGG CATCCAAGCA GCAAGCGCGT TACGCCGTGG
     1081 GTCGATGTTT GATGTTATGG AGCAGCAACG ATGTTACGCA GCAGCAACGA TGTTACGCAG
     1141 CAGGGCAGTC GCCCTAAAAC AAAGTTAGGT GGCTCAAGTA TGGGCATCAT TCGCACATGT
     1201 AGGCTCGGCC CTGACCAAGT CAAATCCATG CGGGCTGCTC TTGATCTTTT CGGTCGTGAG
     1261 TTCGGAGACG TAGCCACCTA CTCCCAACAT CAGCCGGACT CCGATTACCT CGGGAACTTG
     1321 CTCCGTAGTA AGACATTCAT CGCGCTTGCT GCCTTCGACC AAGAAGCGGT TGTTGGCGCT
     1381 CTCGCGGCTT ACGTTCTGCC CAAGTTTGAG CAGCCGCGTA GTGAGATCTA TATCTATGAT
     1441 CTCGCAGTCT CCGGAGAGCA CCGGAGGCAG GGCATTGCCA CCGCGCTCAT CAATCTCCTC
     1501 AAGCATGAGG CCAACGCGCT TGGTGCTTAT GTGATCTACG TGCAAGCAGA TTACGGTGAC
     1561 GATCCCGCAG TGGCTCTCTA TACAAAGTTG GGCATACGGG AAGAAGTGAT GCACTTTGAT
     1621 ATCGACCCAA GTACCGCCAC CTAACAATTC GTTCAAGCCG AGATCGGCTT CCCGGCCGCG
     1681 GAGTTGTTCG GTAAATTGGA CAACGGTCCG CGCGTTGTCC TTTTCCGCTG CATAACCCTG
     1741 CTTCGGGGTC ATTATAGCGA TTTTTTCGGT ATATCCATCC TTTTTCGCAC GATATACAGG
     1801 ATTTTGCCAA AGGGTTCGTG TAGACTTTCC TTGGTGTATC CAACGGCGTC AGCCGGGCAG
     1861 GATAGGTGAA GTAGGCCCAC CCGCGAGCGG GTGTTCCTTC TTCACTGTCC CTTATTCGCA
     1921 CCTGGCGGTG CTCAACGGGA ATCCTGCTCT GCGAGGCTGG CCGTAGGCCG GCCCTACCGG
     1981 CGCGGCAGCG TTACCCGTGT CGGCGGCTCC AACGGCTCGC CATCGTCCAG AAAACACGGC
     2041 TCATCGGGCA TCGGCAGGCG CTGCTGCCCG CGCCGTTCCC ATTCCTCCGT TTCGGTCAAG
     2101 GCTGGCAGGT CTGGTTCCAT GCCCGGAATG CCGGGCTGGC TGGGCGGCTC CTCGCCGGGG
     2161 CCGGTCGGTA GTTGCTGCTC GCCCGGATAC AGGGTCGGGA TGCGGCGCAG GTCGCCATGC
     2221 CCCAACAGCG ATTCGTCCTG GTCGTCGTGA TCAACCACCA CGGCGGCACT GAACACCGAC
     2281 AGGCGCAACT GGTCGCGGGG CTGGCCCCAC GCCACGCGGT CATTGACCAC GTAGGCCGAC
     2341 ACGGTGCCGG GGCCGTTGAG CTTCACGACG GAGATCCAGC GCTCGGCCAC CAAGTCCTTG
     2401 ACTGCGTATT GGACCGTCCG CAAAGAACGT CCGATGAGCT TGGAAAGTGT TTTCTGGCTG
     2461 ACCACCACGG CGTTCTGGTG GCCCATCTGC GCCACGAGGT GATGCAGCAG CATTGCCGCC
     2521 GTGGGTTTCC TCGCAATAAG CCCGGCCCAC GCCTCATGCG CTTTGCGTTC CGTTTGCACC
     2581 CAGTGACCGG GCTTGTTCTT GGCTTGAATG CCGATTTCTC TGGACTGCGT GGCCATGCTT
     2641 ATCTCCATGC GGTAGGGGTG CCGCACGGTT GCGGCACCAT GCGCAATCAG CTGCAACTTT
     2701 TCGGCAGCGC GACAACAATT ATGCGTTGCG TAAAAGTGGC AGTCAATTAC AGATTTTCTT
     2761 TAACCTACGC AATGAGCTAT TGCGGGGGGT GCCGCAATGA GCTGTTGCGT ACCCCCCTTT
     2821 TTTAAGTTGT TGATTTTTAA GTCTTTCGCA TTTCGCCCTA TATCTAGTTC TTTGGTGCCC
     2881 AAAGAAGGGC ACCCCTGCGG GGTTCCCCCA CGCCTTCGGC GCGGCTCCCC CTCCGGCAAA
     2941 AAGTGGCCCC TCCGGGGCTT GTTGATCGAC TGCGCGGCCT TCGGCCTTGC CCAAGGTGGC
     3001 GCTGCCCCCT TGGAACCCCC GCACTCGCCG CCGTGAGGCT CGGGGGGCAG GCGGGCGGGC
     3061 TTCGCCCTTC GACTGCCCCC ACTCGCATAG GCTTGGGTCG TTCCAGGCGC GTCAAGGCCA
     3121 AGCCGCTGCG CGGTCGCTGC GCGAGCCTTG ACCCGCCTTC CACTTGGTGT CCAACCGGCA
     3181 AGCGAAGCGC GCAGGCCGCA GGCCGGAGGC TTTTCCCCAG AGAAAATTAA AAAAATTGAT
     3241 GGGGCAAGGC CGCAGGCCGC GCAGTTGGAG CCGGTGGGTA TGTGGTCGAA GGCTGGGTAG
     3301 CCGGTGGGCA ATCCCTGTGG TCAAGCTCGT GGGCAGGCGC AGCCTGTCCA TCAGCTTGTC
     3361 CAGCAGGGTT GTCCACGGGC CGAGCGAAGC GAGCCAGCCG GTGGCCGCTC GCGGCCATCG
     3421 TCCACATATC CACGGGCTGG CAAGGGAGCG CAGCGACCGC GCAGGGCGAA GCCCGGAGAG
     3481 CAAGCCCGTA GGGGGGGCGC GCCCAGCTGT CTAGGGCGGC GGATTTGTCC TACTCAGGAG
     3541 AGCGTTCACC GACAAACAAC AGATAAAACG AAAGGCCCAG TCTTTCGACT GAGCCTTTCG
     3601 TTTTATTTGA TGC    
//