LOCUS       Untitled_Sequence        4449 bp    DNA     circular SYN 29-JUL-2022
FEATURES             Location/Qualifiers
     primer_bind     339..355
                     /label="M13 fwd"
                     /color="#a020f0"
     primer_bind     253..269
                     /label="M13 rev"
                     /color="#a020f0"
     protein_bind    229..245
                     /label="lac operator"
                     /color="#31849b"
     protein_bind    155..176
                     /label="CAP binding site"
                     /color="#31849b"
     promoter        215..221
                     /label="lac promoter"
                     /color="#ffffff"
     misc_feature    282..338
                     /label="MCS"
                     /color="#99ccff"
     terminator      781..875
                     /label="lambda t0 terminator"
                     /color="#ffffff"
     oriT            1989..2097
                     /label="oriT"
                     /color="#a6acb3"
     CDS             265..588
                     /label="lacZα"
                     /color="#993366"
     CDS             1007..1822
                     /label="KanR"
                     /color="#ccffcc"
     CDS             2158..3306
                     /label="trfA"
                     /color="#993366"
ORIGIN      
        1 TTAATTAAAG CGGATAACAA TTTCACACAG GAGGCCGCCT AGGCCGCGGC CGCGCGAATT
       61 CGAGCTCGGT ACCCGGGAAG ACAATGCCGG AGTGAGACCG CAGCTGGCAC GACAGGTTTC
      121 CCGACTGGAA AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG
      181 CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT
      241 AACAATTTCA CACAGGAAAC AGCTATGACC ATGATTACGC CAAGCTTGCA TGCCTGCAGG
      301 TCGACTCTAG AGGATCCCCG GGTACCGAGC TCGAATTCAC TGGCCGTCGT TTTACAACGT
      361 CGTGACTGGG AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA TCCCCCTTTC
      421 GCCAGCTGGC GTAATAGCGA AGAGGCCCGC ACCGATCGCC CTTCCCAACA GTTGCGCAGC
      481 CTGAATGGCG AATGGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA
      541 CACCGCATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGCCC
      601 CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT
      661 TACAGACAAG CTGTGACGGT CTCACGCTGC AATTGTCTTC GGGGATCCTC TAGAGTCGAC
      721 CTGCAGGCAT GCAAGCTTGC GGCCGCGTCG TGACTGGGAA AACCCTGGCG ACTAGTCTTG
      781 GACTCCTGTT GATAGATCCA GTAATGACCT CAGAACTCCA TCTGGATTTG TTCAGAACGC
      841 TCGGTTGCCG CCGGGCGTTT TTTATTGGTG AGAATCCAGG GGTCCCCAAT AATTACGATT
      901 TAAATTTGTG TCTCAAAATC TCTGATGTTA CATTGCACAA GATAAAAATA TATCATCATG
      961 AACAATAAAA CTGTCTGCTT ACATAAACAG TAATACAAGG GGTGTTATGA GCCATATTCA
     1021 GCGTGAAACG AGCTGTAGCC GTCCGCGTCT GAACAGCAAC ATGGATGCGG ATCTGTATGG
     1081 CTATAAATGG GCGCGTGATA ACGTGGGTCA GAGCGGCGCG ACCATTTATC GTCTGTATGG
     1141 CAAACCGGAT GCGCCGGAAC TGTTTCTGAA ACATGGCAAA GGCAGCGTGG CGAACGATGT
     1201 GACCGATGAA ATGGTGCGTC TGAACTGGCT GACCGAATTT ATGCCGCTGC CGACCATTAA
     1261 ACATTTTATT CGCACCCCGG ATGATGCGTG GCTGCTGACC ACCGCGATTC CGGGCAAAAC
     1321 CGCGTTTCAG GTGCTGGAAG AATATCCGGA TAGCGGCGAA AACATTGTGG ATGCGCTGGC
     1381 CGTGTTTCTG CGTCGTCTGC ATAGCATTCC GGTGTGCAAC TGCCCGTTTA ACAGCGATCG
     1441 TGTGTTTCGT CTGGCCCAGG CGCAGAGCCG TATGAACAAC GGCCTGGTGG ATGCGAGCGA
     1501 TTTTGATGAT GAACGTAACG GCTGGCCGGT GGAACAGGTG TGGAAAGAAA TGCATAAACT
     1561 GCTGCCGTTT AGCCCGGATA GCGTGGTGAC CCACGGCGAT TTTAGCCTGG ATAACCTGAT
     1621 TTTCGATGAA GGCAAACTGA TTGGCTGCAT TGATGTGGGC CGTGTGGGCA TTGCGGATCG
     1681 TTATCAGGAT CTGGCCATTC TGTGGAACTG CCTGGGCGAA TTTAGCCCGA GCCTGCAAAA
     1741 ACGTCTGTTT CAGAAATATG GCATTGATAA TCCGGATATG AACAAACTGC AATTTCATCT
     1801 GATGCTGGAT GAATTTTTCT AATAATTAAT TGGACCGCGG TCCGCGCGTT GTCCTTTTCC
     1861 GCTGCATAAC CCTGCTTCGG GGTCATTATA GCGATTTTTT CGGTATATCC ATCCTTTTTC
     1921 GCACGATATA CAGGATTTTG CCAAAGGGTT CGTGTAGACT TTCCTTGGTG TATCCAACGG
     1981 CGTCAGCCGG GCAGGATAGG TGAAGTAGGC CCACCCGCGA GCGGGTGTTC CTTCTTCACT
     2041 GTCCCTTATT CGCACCTGGC GGTGCTCAAC GGGAATCCTG CTCTGCGAGG CTGGCCGTAG
     2101 GCCGGCCGCG ATGCAGGTGG CTGCTGAACC CCCAGCCGGA ACTGACCCCA CAAGGCCCTA
     2161 GCGTTTGCAA TGCACCAGGT CATCATTGAC CCAGGCGTGT TCCACCAGGC CGCTGCCTCG
     2221 CAACTCTTCG CAGGCTTCGC CGACCTGCTC GCGCCACTTC TTCACGCGGG TGGAATCCGA
     2281 TCCGCACATG AGGCGGAAGG TTTCCAGCTT GAGCGGGTAC GGCTCCCGGT GCGAGCTGAA
     2341 ATAGTCGAAC ATCCGTCGGG CCGTCGGCGA CAGCTTGCGG TACTTCTCCC ATATGAATTT
     2401 CGTGTAGTGG TCGCCAGCAA ACAGCACGAC GATTTCCTCG TCGATCAGGA CCTGGCAACG
     2461 GGACGTTTTC TTGCCACGGT CCAGGACGCG GAAGCGGTGC AGCAGCGACA CCGATTCCAG
     2521 GTGCCCAACG CGGTCGGACG TGAAGCCCAT CGCCGTCGCC TGTAGGCGCG ACAGGCATTC
     2581 CTCGGCCTTC GTGTAATACC GGCCATTGAT CGACCAGCCC AGGTCCTGGC AAAGCTCGTA
     2641 GAACGTGAAG GTGATCGGCT CGCCGATAGG GGTGCGCTTC GCGTACTCCA ACACCTGCTG
     2701 CCACACCAGT TCGTCATCGT CGGCCCGCAG CTCGACGCCG GTGTAGGTGA TCTTCACGTC
     2761 CTTGTTGACG TGGAAAATGA CCTTGTTTTG CAGCGCCTCG CGCGGGATTT TCTTGTTGCG
     2821 CGTGGTGAAC AGGGCAGAGC GGGCCGTGTC GTTTGGCATC GCTCGCATCG TGTCCGGCCA
     2881 CGGCGCAATA TCGAACAAGG AAAGCTGCAT TTCCTTGATC TGCTGCTTCG TGTGTTTCAG
     2941 CAACGCGGCC TGCTTGGCTT CGCTGACCTG TTTTGCCAGG TCCTCGCCGG CGGTTTTTCG
     3001 CTTCTTGGTC GTCATAGTTC CTCGCGTGTC GATGGTCATC GACTTCGCCA AACCTGCCGC
     3061 CTCCTGTTCG AGACGACGCG AACGCTCCAC GGCGGCCGAT GGCGCGGGCA GGGCAGGGGG
     3121 AGCCAGTTGC ACGCTGTCGC GCTCGATCTT GGCCGTAGCT TGCTGGACTA TCGAGCCGAC
     3181 GGACTGGAAG GTTTCGCGGG GCGCACGCAT GACGGTGCGG CTTGCGATGG TTTCGGCATC
     3241 CTCGGCGGAA AACCCCGCGT CGATCAGTTC TTGCCTGTAT GCCTTCCGGT CAAACGTCCG
     3301 ATTCATTCAC CCTCCTTGCG GGATTGCCCC GGAATTAATT CCCCGGATCG ATCCGTCGAT
     3361 CTTGATCCCC TGCGCCATCA GATCCTTGGC GGCAAGAAAG CCATCCAGTT TACTTTGCAG
     3421 GGCTTCCCAA CCTTACCAGA GGGCGCCCCA GCTGGCAATT CCGGTTCGCT TGCTGTCCAT
     3481 AAAACCGCCC AGTCTAGCTA TCGCCATGTA AGCCCACTGC AAGCTACCTG CTTTCTCTTT
     3541 GCGCTTGCGT TTTCCCTTGT CCAGATAGCC CAGTAGCTGA CATTCATCCG GGGTCAGCAC
     3601 CGTTTCTGCG GACTGGCTTT CTACGTGGCT GCCATTTTTG GGGTGAGGCC GTTCGCGGCC
     3661 GAGGGGCGCA GCCCCTGGGG GGATGGGAGG CCCGCGTTAG CGGGCCGGGA GGGTTCGAGA
     3721 AGGGGGGGCA CCCCCCTTCG GCGTGCGCGG TCACGCGCAC AGGGCGCAGC CCTGGTTAAA
     3781 AACAAGGTTT ATAAATATTG GTTTAAAAGC AGGTTAAAAG ACAGGTTAGC GGTGGCCGAA
     3841 AAACGGGCGG AAACCCTTGC AAATGCTGGA TTTTCTGCCT GTGGACAGCC CCTCAAATGT
     3901 CAATAGGTGC GCCCCTCATC TGTCAGCACT CTGCCCCTCA AGTGTCAAGG ATCGCGCCCC
     3961 TCATCTGTCA GTAGTCGCGC CCCTCAAGTG TCAATACCGC AGGGCACTTA TCCCCAGGCT
     4021 TGTCCACATC ATCTGTGGGA AACTCGCGTA AAATCAGGCG TTTTCGCCGA TTTGCGAGGC
     4081 TGGCCAGCTC CACGTCGCCG GCCGAAATCG AGCCTGCCCC TCATCTGTCA ACGCCGCGCC
     4141 GGGTGAGTCG GCCCCTCAAG TGTCAACGTC CGCCCCTCAT CTGTCAGTGA GGGCCAAGTT
     4201 TTCCGCGAGG TATCCACAAC GCCGGCGGCC CTACATGGCT CTGCTGTAGT GAGTGGGTTG
     4261 CGCTCCGGCA GCGGTCCTGA TCCCCCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT
     4321 CTTTTCTACG GCGCGCCCAG CTGTCTAGGG CGGCGGATTT GTCCTACTCA GGAGAGCGTT
     4381 CACCGACAAA CAACAGATAA AACGAAAGGC CCAGTCTTTC GACTGAGCCT TTCGTTTTAT
     4441 TTGATGCCT     
//
