LOCUS       Untitled_Sequence        3022 bp    DNA     circular SYN 28-FEB-2023
FEATURES             Location/Qualifiers
     primer_bind     329..345
                     /label="M13 fwd"
                     /color="#a020f0"
     primer_bind     243..259
                     /label="M13 rev"
                     /color="#a020f0"
     protein_bind    219..235
                     /label="lac operator"
                     /color="#31849b"
     protein_bind    2877..2898
                     /label="CAP binding site"
                     /color="#31849b"
     protein_bind    145..166
                     /label="CAP binding site"
                     /color="#31849b"
     promoter        919..924
                     /label="Pc promoter"
                     /color="#ffffff"
     promoter        205..211
                     /label="lac promoter"
                     /color="#ffffff"
     misc_feature    272..328
                     /label="MCS"
                     /color="#99ccff"
     terminator      771..865
                     /label="lambda t0 terminator"
                     /color="#ffffff"
     oriT            1857..1965
                     /label="oriT"
                     /color="#a6acb3"
     CDS             255..578
                     /label="lacZα"
                     /color="#993366"
     CDS             1113..1646
                     /label="GmR"
                     /color="#ccffcc"
     rep_origin      2001..2589
                     /label="ori"
                     /color="#ffff00"
     terminator      2932..3018
                     /label="rrnB T1 terminator"
                     /color="#ffffff"
     misc_feature    87..90
                     /label="1"
                     /color="#99cc00"
     misc_feature    83..86
                     /label="A"
                     /color="#00ffff"
     misc_feature    675..678
                     /label="3"
                     /color="#99cc00"
     misc_feature    679..682
                     /label="B"
                     /color="#00ffff"
ORIGIN      
        1 TTAATTAAAG CGGATAACAA TTTCACACAG GAGGCCGCCT AGGCCGCGGC CGCGCGAATT
       61 CGAGCTCGGT ACCCGGGTCT CAGGAGTGCC TTGTCTTCAC GACAGGTTTG CCGACTGGAA
      121 AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC
      181 TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA
      241 CACAGGAAAC AGCTATGACC ATGATTACGC CAAGCTTGCA TGCCTGCAGG TCGACTCTAG
      301 AGGATCCCCG GGTACCGAGC TCGAATTCAC TGGCCGTCGT TTTACAACGT CGTGACTGGG
      361 AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA TCCCCCTTTC GCCAGCTGGC
      421 GTAATAGCGA AGAGGCCCGC ACCGATCGCC CTTCCCAACA GTTGCGCAGC CTGAATGGCG
      481 AATGGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT
      541 GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGCCC CGACACCCGC
      601 CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG
      661 CTGTGAGAAG ACAAACTATA CTTGAGACCC GGGGATCCTC TAGAGTCGAC CTGCAGGCAT
      721 GCAAGCTTGC GGCCGCGTCG TGACTGGGAA AACCCTGGCG ACTAGTCTTG GACTCCTGTT
      781 GATAGATCCA GTAATGACCT CAGAACTCCA TCTGGATTTG TTCAGAACGC TCGGTTGCCG
      841 CCGGGCGTTT TTTATTGGTG AGAATCCAGG GGTCCCCAAT AATTACGATT TAAATTTGAC
      901 ATAAGCCTGT TCGGTTCGTA AACTGTAATG CAAGTAGCGT ATGCGCTCAC GCAACTGGTC
      961 CAGAACCTTG ACCGAACGCA GCGGTGGTAA CGGCGCAGTG GCGGTTTTCA TGGCTTGTTA
     1021 TGACTGTTTT TTTGTACAGC CTATGCCTCG GGCATCCAAG CAGCAAGCGC GTTACGCCGT
     1081 GGGTCGATGT TTGATGTTAT GGAGCAGCAA CGATGTTACG CAGCAGCAAC GATGTTACGC
     1141 AGCAGGGCAG TCGCCCTAAA ACAAAGTTAG GTGGCTCAAG TATGGGCATC ATTCGCACAT
     1201 GTAGGCTCGG CCCTGACCAA GTCAAATCCA TGCGGGCTGC TCTTGATCTT TTCGGTCGTG
     1261 AGTTCGGAGA CGTAGCCACC TACTCCCAAC ATCAGCCGGA CTCCGATTAC CTCGGGAACT
     1321 TGCTCCGTAG TAAGACATTC ATCGCGCTTG CTGCCTTCGA CCAAGAAGCG GTTGTTGGCG
     1381 CTCTCGCGGC TTACGTTCTG CCCAAGTTTG AGCAGCCGCG TAGTGAGATC TATATCTATG
     1441 ATCTCGCAGT CTCCGGAGAG CACCGGAGGC AGGGCATTGC CACCGCGCTC ATCAATCTCC
     1501 TCAAGCATGA GGCCAACGCG CTTGGTGCTT ATGTGATCTA CGTGCAAGCA GATTACGGTG
     1561 ACGATCCCGC AGTGGCTCTC TATACAAAGT TGGGCATACG GGAAGAAGTG ATGCACTTTG
     1621 ATATCGACCC AAGTACCGCC ACCTAACAAT TCGTTCAAGC CGAGATCGGC TTCCCGGCCG
     1681 CGGAGTTGTT CGGTAAATTG GACAACGGTC CGCGCGTTGT CCTTTTCCGC TGCATAACCC
     1741 TGCTTCGGGG TCATTATAGC GATTTTTTCG GTATATCCAT CCTTTTTCGC ACGATATACA
     1801 GGATTTTGCC AAAGGGTTCG TGTAGACTTT CCTTGGTGTA TCCAACGGCG TCAGCCGGGC
     1861 AGGATAGGTG AAGTAGGCCC ACCCGCGAGC GGGTGTTCCT TCTTCACTGT CCCTTATTCG
     1921 CACCTGGCGG TGCTCAACGG GAATCCTGCT CTGCGAGGCT GGCCGTAGGC CGGCCCCGTA
     1981 GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA
     2041 ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT
     2101 TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTTCT TCTAGTGTAG
     2161 CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA
     2221 ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA
     2281 AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG
     2341 CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTTTGAGAA
     2401 AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA
     2461 ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC
     2521 GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC
     2581 CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT
     2641 GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT
     2701 GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG
     2761 GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA
     2821 TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT
     2881 GTGAGTTAGC TCACTCATTA GGCAGGCGCG CCCAGCTGTC TAGGGCGGCG GATTTGTCCT
     2941 ACTCAGGAGA GCGTTCACCG ACAAACAACA GATAAAACGA AAGGCCCAGT CTTTCGACTG
     3001 AGCCTTTCGT TTTATTTGAT GC   
//