LOCUS       Untitled_Sequence        3156 bp    DNA     circular SYN 29-JUL-2022
FEATURES             Location/Qualifiers
     CDS             1125..1940
                     /label="KanR"
                     /color="#ccffcc"
                     /color="#ffff00"
     CDS             383..706
                     /label="lacZα"
                     /color="#993366"
     terminator      899..993
                     /label="lambda t0 terminator"
                     /color="#ffffff"
     terminator      26..112
                     /label="rrnB T1 terminator"
                     /color="#ffffff"
     misc_feature    400..456
                     /label="MCS"
                     /color="#99ccff"
     promoter        333..339
                     /label="lac promoter"
                     /color="#ffffff"
     protein_bind    273..294
                     /label="CAP binding site"
                     /color="#31849b"
     protein_bind    3127..3148
                     /label="CAP binding site"
                     /color="#31849b"
     protein_bind    347..363
                     /label="lac operator"
                     /color="#31849b"
     primer_bind     371..387
                     /label="M13 rev"
                     /color="#a020f0"
     primer_bind     457..473
                     /label="M13 fwd"
                     /color="#a020f0"
ORIGIN      
        1 CGCGCCCAGC TGTCTAGGGC GGCGGATTTG TCCTACTCAG GAGAGCGTTC ACCGACAAAC
       61 AACAGATAAA ACGAAAGGCC CAGTCTTTCG ACTGAGCCTT TCGTTTTATT TGATGCCTTT
      121 AATTAAAGCG GATAACAATT TCACACAGGA GGCCGCCTAG GCCGCGGCCG CGCGAATTCG
      181 AGCTCGGTAC CCGGGAAGAC AACAGAGGAG TGAGACCGCA GCTGGCACGA CAGGTTTCCC
      241 GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA
      301 CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA
      361 CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGCCA AGCTTGCATG CCTGCAGGTC
      421 GACTCTAGAG GATCCCCGGG TACCGAGCTC GAATTCACTG GCCGTCGTTT TACAACGTCG
      481 TGACTGGGAA AACCCTGGCG TTACCCAACT TAATCGCCTT GCAGCACATC CCCCTTTCGC
      541 CAGCTGGCGT AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT TGCGCAGCCT
      601 GAATGGCGAA TGGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA
      661 CCGCATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGCCCCG
      721 ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA
      781 CAGACAAGCT GTGACGGTCT CACGCTTGTG TTGTCTTCGG GGATCCTCTA GAGTCGACCT
      841 GCAGGCATGC AAGCTTGCGG CCGCGTCGTG ACTGGGAAAA CCCTGGCGAC TAGTCTTGGA
      901 CTCCTGTTGA TAGATCCAGT AATGACCTCA GAACTCCATC TGGATTTGTT CAGAACGCTC
      961 GGTTGCCGCC GGGCGTTTTT TATTGGTGAG AATCCAGGGG TCCCCAATAA TTACGATTTA
     1021 AATTTGTGTC TCAAAATCTC TGATGTTACA TTGCACAAGA TAAAAATATA TCATCATGAA
     1081 CAATAAAACT GTCTGCTTAC ATAAACAGTA ATACAAGGGG TGTTATGAGC CATATTCAGC
     1141 GTGAAACGAG CTGTAGCCGT CCGCGTCTGA ACAGCAACAT GGATGCGGAT CTGTATGGCT
     1201 ATAAATGGGC GCGTGATAAC GTGGGTCAGA GCGGCGCGAC CATTTATCGT CTGTATGGCA
     1261 AACCGGATGC GCCGGAACTG TTTCTGAAAC ATGGCAAAGG CAGCGTGGCG AACGATGTGA
     1321 CCGATGAAAT GGTGCGTCTG AACTGGCTGA CCGAATTTAT GCCGCTGCCG ACCATTAAAC
     1381 ATTTTATTCG CACCCCGGAT GATGCGTGGC TGCTGACCAC CGCGATTCCG GGCAAAACCG
     1441 CGTTTCAGGT GCTGGAAGAA TATCCGGATA GCGGCGAAAA CATTGTGGAT GCGCTGGCCG
     1501 TGTTTCTGCG TCGTCTGCAT AGCATTCCGG TGTGCAACTG CCCGTTTAAC AGCGATCGTG
     1561 TGTTTCGTCT GGCCCAGGCG CAGAGCCGTA TGAACAACGG CCTGGTGGAT GCGAGCGATT
     1621 TTGATGATGA ACGTAACGGC TGGCCGGTGG AACAGGTGTG GAAAGAAATG CATAAACTGC
     1681 TGCCGTTTAG CCCGGATAGC GTGGTGACCC ACGGCGATTT TAGCCTGGAT AACCTGATTT
     1741 TCGATGAAGG CAAACTGATT GGCTGCATTG ATGTGGGCCG TGTGGGCATT GCGGATCGTT
     1801 ATCAGGATCT GGCCATTCTG TGGAACTGCC TGGGCGAATT TAGCCCGAGC CTGCAAAAAC
     1861 GTCTGTTTCA GAAATATGGC ATTGATAATC CGGATATGAA CAAACTGCAA TTTCATCTGA
     1921 TGCTGGATGA ATTTTTCTAA TAATTAATTG GACCGCGGTC CGCGCGTTGT CCTTTTCCGC
     1981 TGCATAACCC TGCTTCGGGG TCATTATAGC GATTTTTTCG GTATATCCAT CCTTTTTCGC
     2041 ACGATATACA GGATTTTGCC AAAGGGTTCG TGTAGACTTT CCTTGGTGTA TCCAACGGCG
     2101 TCAGCCGGGC AGGATAGGTG AAGTAGGCCC ACCCGCGAGC GGGTGTTCCT TCTTCACTGT
     2161 CCCTTATTCG CACCTGGCGG TGCTCAACGG GAATCCTGCT CTGCGAGGCT GGCCGTAGGC
     2221 CGGCCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG
     2281 CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT
     2341 ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTTCT
     2401 TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT
     2461 CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG
     2521 GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC
     2581 GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA
     2641 GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG
     2701 CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA
     2761 TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG
     2821 GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG
     2881 CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT
     2941 TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC
     3001 AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC
     3061 GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA
     3121 CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCAGG  
//
