LOCUS       Untitled_Sequence        4317 bp    DNA     circular SYN 28-FEB-2023
FEATURES             Location/Qualifiers
     CDS             2026..3174
                     /label="trfA"
                     /color="#993366"
     CDS             1113..1646
                     /label="GmR"
                     /color="#ccffcc"
     rep_origin      3567..4098
                     /label="oriV"
                     /color="#ffff00"
     oriT            1857..1966
                     /label="oriT"
                     /color="#a6acb3"
     terminator      771..865
                     /label="lambda t0 terminator"
                     /color="#ffffff"
     terminator      4225..4311
                     /label="rrnB T1 terminator"
                     /color="#ffffff"
     promoter        919..924
                     /label="Pc promoter"
                     /color="#ffffff"
     primer_bind     329..345
                     /label="M13 fwd"
                     /color="#a020f0"
     primer_bind     243..259
                     /label="M13 rev"
                     /color="#a020f0"
     protein_bind    219..235
                     /label="lac operator"
                     /color="#31849b"
     protein_bind    145..166
                     /label="CAP binding site"
                     /color="#31849b"
     promoter        205..211
                     /label="lac promoter"
                     /color="#ffffff"
     misc_feature    272..328
                     /label="MCS"
                     /color="#99ccff"
     CDS             255..578
                     /label="lacZα"
                     /color="#993366"
     misc_feature    83..86
                     /label="A"
                     /color="#00ffff"
     misc_feature    87..90
                     /label="1"
                     /color="#99cc00"
     misc_feature    675..678
                     /label="3"
                     /color="#99cc00"
     misc_feature    679..682
                     /label="B"
                     /color="#00ffff"
ORIGIN      
        1 TTAATTAAAG CGGATAACAA TTTCACACAG GAGGCCGCCT AGGCCGCGGC CGCGCGAATT
       61 CGAGCTCGGT ACCCGGGTCT CAGGAGTGCC TTGTCTTCAC GACAGGTTTG CCGACTGGAA
      121 AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC
      181 TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA
      241 CACAGGAAAC AGCTATGACC ATGATTACGC CAAGCTTGCA TGCCTGCAGG TCGACTCTAG
      301 AGGATCCCCG GGTACCGAGC TCGAATTCAC TGGCCGTCGT TTTACAACGT CGTGACTGGG
      361 AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA TCCCCCTTTC GCCAGCTGGC
      421 GTAATAGCGA AGAGGCCCGC ACCGATCGCC CTTCCCAACA GTTGCGCAGC CTGAATGGCG
      481 AATGGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT
      541 GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGCCC CGACACCCGC
      601 CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG
      661 CTGTGAGAAG ACAAACTATA CTTGAGACCC GGGGATCCTC TAGAGTCGAC CTGCAGGCAT
      721 GCAAGCTTGC GGCCGCGTCG TGACTGGGAA AACCCTGGCG ACTAGTcttg gactcctgtt
      781 gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc tcggttgccg
      841 ccgggcgttt tttattggtg agaatccagg ggtccccaat aattacgatt taaatttgac
      901 ataagcctgt tcggttcgta aactgtaatg caagtagcgt atgcgctcac gcaactggtc
      961 cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta
     1021 tgactgtttt tttgtacagc ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt
     1081 gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagcaac gatgttacgc
     1141 agcagggcag tcgccctaaa acaaagttag gtggctcaag tatgggcatc attcgcacat
     1201 gtaggctcgg ccctgaccaa gtcaaatcca tgcgggctgc tcttgatctt ttcggtcgtg
     1261 agttcggaga cgtagccacc tactcccaac atcagccgga ctccgattac ctcgggaact
     1321 tgctccgtag taagacattc atcgcgcttg ctgccttcga ccaagaagcg gttgttggcg
     1381 ctctcgcggc ttacgttctg cccaagtttg agcagccgcg tagtgagatc tatatctatg
     1441 atctcgcagt ctccggagag caccggaggc agggcattgc caccgcgctc atcaatctcc
     1501 tcaagcatga ggccaacgcg cttggtgctt atgtgatcta cgtgcaagca gattacggtg
     1561 acgatcccgc agtggctctc tatacaaagt tgggcatacg ggaagaagtg atgcactttg
     1621 atatcgaccc aagtaccgcc acctaacaat tcgttcaagc cgagatcggc ttcccggccg
     1681 cggagttgtt cggtaaattg gacaacggtc cgcgcgttgt ccttttccgc tgcataaccc
     1741 tgcttcgggg tcattatagc gattttttcg gtatatccat cctttttcgc acgatataca
     1801 ggattttgcc aaagggttcg tgtagacttt ccttggtgta tccaacggcg tcagccgggc
     1861 aggataggtg aagtaggccc acccgcgagc gggtgttcct tcttcactgt cccttattcg
     1921 cacctggcgg tgctcaacgg gaatcctgct ctgcgaggct ggccgtaggc cggccgcgat
     1981 gcaggtggct gctgaacccc cagccggaac tgaccccaca aggccctagc gtttgcaatg
     2041 caccaggtca tcattgaccc aggcgtgttc caccaggccg ctgcctcgca actcttcgca
     2101 ggcttcgccg acctgctcgc gccacttctt cacgcgggtg gaatccgatc cgcacatgag
     2161 gcggaaggtt tccagcttga gcgggtacgg ctcccggtgc gagctgaaat agtcgaacat
     2221 ccgtcgggcc gtcggcgaca gcttgcggta cttctcccat atgaatttcg tgtagtggtc
     2281 gccagcaaac agcacgacga tttcctcgtc gatcaggacc tggcaacggg acgttttctt
     2341 gccacggtcc aggacgcgga agcggtgcag cagcgacacc gattccaggt gcccaacgcg
     2401 gtcggacgtg aagcccatcg ccgtcgcctg taggcgcgac aggcattcct cggccttcgt
     2461 gtaataccgg ccattgatcg accagcccag gtcctggcaa agctcgtaga acgtgaaggt
     2521 gatcggctcg ccgatagggg tgcgcttcgc gtactccaac acctgctgcc acaccagttc
     2581 gtcatcgtcg gcccgcagct cgacgccggt gtaggtgatc ttcacgtcct tgttgacgtg
     2641 gaaaatgacc ttgttttgca gcgcctcgcg cgggattttc ttgttgcgcg tggtgaacag
     2701 ggcagagcgg gccgtgtcgt ttggcatcgc tcgcatcgtg tccggccacg gcgcaatatc
     2761 gaacaaggaa agctgcattt ccttgatctg ctgcttcgtg tgtttcagca acgcggcctg
     2821 cttggcttcg ctgacctgtt ttgccaggtc ctcgccggcg gtttttcgct tcttggtcgt
     2881 catagttcct cgcgtgtcga tggtcatcga cttcgccaaa cctgccgcct cctgttcgag
     2941 acgacgcgaa cgctccacgg cggccgatgg cgcgggcagg gcagggggag ccagttgcac
     3001 gctgtcgcgc tcgatcttgg ccgtagcttg ctggactatc gagccgacgg actggaaggt
     3061 ttcgcggggc gcacgcatga cggtgcggct tgcgatggtt tcggcatcct cggcggaaaa
     3121 ccccgcgtcg atcagttctt gcctgtatgc cttccggtca aacgtccgat tcattcaccc
     3181 tccttgcggg attgccccgg aattaattcc ccggatcgat ccgtcgatct tgatcccctg
     3241 cgccatcaga tccttggcgg caagaaagcc atccagttta ctttgcaggg cttcccaacc
     3301 ttaccagagg gcgccccagc tggcaattcc ggttcgcttg ctgtccataa aaccgcccag
     3361 tctagctatc gccatgtaag cccactgcaa gctacctgct ttctctttgc gcttgcgttt
     3421 tcccttgtcc agatagccca gtagctgaca ttcatccggg gtcagcaccg tttctgcgga
     3481 ctggctttct acgtggctgc catttttggg gtgaggccgt tcgcggccga ggggcgcagc
     3541 ccctgggggg atgggaggcc cgcgttagcg ggccgggagg gttcgagaag ggggggcacc
     3601 ccccttcggc gtgcgcggtc acgcgcacag ggcgcagccc tggttaaaaa caaggtttat
     3661 aaatattggt ttaaaagcag gttaaaagac aggttagcgg tggccgaaaa acgggcggaa
     3721 acccttgcaa atgctggatt ttctgcctgt ggacagcccc tcaaatgtca ataggtgcgc
     3781 ccctcatctg tcagcactct gcccctcaag tgtcaaggat cgcgcccctc atctgtcagt
     3841 agtcgcgccc ctcaagtgtc aataccgcag ggcacttatc cccaggcttg tccacatcat
     3901 ctgtgggaaa ctcgcgtaaa atcaggcgtt ttcgccgatt tgcgaggctg gccagctcca
     3961 cgtcgccggc cgaaatcgag cctgcccctc atctgtcaac gccgcgccgg gtgagtcggc
     4021 ccctcaagtg tcaacgtccg cccctcatct gtcagtgagg gccaagtttt ccgcgaggta
     4081 tccacaacgc cggcggccct acatggctct gctgtagtga gtgggttgcg ctccggcagc
     4141 ggtcctgatc ccccgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggc
     4201 gcgcccagct gtctagggcg gcggatttgt cctactcagg agagcgttca ccgacaaaca
     4261 acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt gatgcct
//