LOCUS       pSEVA6819[gD]           3023 bp ds-DNA     circular     22-SEP-2022
DEFINITION  .
KEYWORDS    "creator:Centro Nacional De Biotecnologia" "marker:GmR"
FEATURES             Location/Qualifiers
     misc_feature    73..1001
                     /label="pUC Ori"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    98..686
                     /label="pUC Ori"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     protein_bind    974..995
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: E. coli catabolite activator protein
note: CAP binding activates transcription in the presence of cAMP."
     terminator      1029..1115
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="gene: Escherichia coli rrnB
note: transcription terminator T1 from the E. coli rrnB gene"
     primer          1197..1239
                     /label="Primer D1"
                     /note="sequence: GGTCTCaAATGTGCCttGTCTTCacgacaggtttgccgactgg"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    1204..1207
                     /label="D"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1208..1211
                     /label=1
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     protein_bind    1266..1287
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     promoter        1302..1307
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        1308..1325
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        1326..1332
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind    1340..1356
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     primer_bind     1364..1380
                     /label="M13 rev"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
     CDS             1376..1699
                     /label="lacZα"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     misc_feature    1393..1449
                     /label="MCS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     complement(1450..1466)
                     /label="M13 fwd"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
     primer          complement(1763..1810)
                     /label="Primer 6E"
                     /note="sequence: GGTCTCaACCTCACAttGTCTTCtcacagcttgtctgtaagcggatgc"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    1796..1799
                     /label=6
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    1800..1803
                     /label="E"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     terminator      1891..1985
                     /label="lambda t0 terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="gene: 
note: transcription terminator from phage lambda"
     promoter        2016..2021
                     /label="Pc promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="gene: intI1 (promoter lies within the coding sequence)
note: class 1 integron promoter"
     promoter        2022..2038
                     /label="Pc promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="gene: intI1 (promoter lies within the coding sequence)
note: class 1 integron promoter"
     promoter        2039..2044
                     /label="Pc promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="gene: intI1 (promoter lies within the coding sequence)
note: class 1 integron promoter"
     CDS             2233..2766
                     /label="GmR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="allele: 
codon_start: 1
gene: aacC1
note: confers resistance to gentamycin
product: gentamycin acetyltransferase
transl_table: 1
translation: MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALDLFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRSEIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIREEVMHFDIDPSTAT*"
     oriT            2977..62
                     /label="oriT"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: incP origin of transfer"
ORIGIN
        1 TCACTGTCCC TTATTCGCAC CTGGCGGTGC TCAACGGGAA TCCTGCTCTG CGAGGCTGGC
       61 CGTAGGCCGG CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG
      121 TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC
      181 AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA
      241 CTGTTCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA
      301 CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC
      361 TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG
      421 GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC
      481 AGCGTGAGCT TTGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG
      541 TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT
      601 ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT
      661 CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG
      721 CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA
      781 ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA
      841 GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCCAATACG CAAACCGCCT CTCCCCGCGC
      901 GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA GCGGGCAGTG
      961 AGCGCAACGC AATTAATGTG AGTTAGCTCA CTCATTAGGC AGGCGCGCCC AGCTGTCTAG
     1021 GGCGGCGGAT TTGTCCTACT CAGGAGAGCG TTCACCGACA AACAACAGAT AAAACGAAAG
     1081 GCCCAGTCTT TCGACTGAGC CTTTCGTTTT ATTTGATGCC TTTAATTAAA GCGGATAACA
     1141 ATTTCACACA GGAGGCCGCC TAGGCCGCGG CCGCGCGAAT TCGAGCTCGG TACCCgGGTC
     1201 TCaAATGTGC CttGTCTTCa cgacaggttt gccgactgga aagcgggcag tgagcgcaac
     1261 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     1321 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     1381 catgattacg ccaagcttgc atgcctgcag gtcgactcta gaggatcccc gggtaccgag
     1441 ctcgaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     1501 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     1561 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta
     1621 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat
     1681 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     1741 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaGAA GACaaTGTGA
     1801 GGTtGAGACC GGGGATCCTC TAGAGTCGAC CTGCAGGCAT GCAAGCTTGC GGCCGCGTCG
     1861 TGACTGGGAA AACCCTGGCG ACTAGTCTTG GACTCCTGTT GATAGATCCA GTAATGACCT
     1921 CAGAACTCCA TCTGGATTTG TTCAGAACGC TCGGTTGCCG CCGGGCGTTT TTTATTGGTG
     1981 AGAATCCAGG GGTCCCCAAT AATTACGATT TAAATTTGAC ATAAGCCTGT TCGGTTCGTA
     2041 AACTGTAATG CAAGTAGCGT ATGCGCTCAC GCAACTGGTC CAGAACCTTG ACCGAACGCA
     2101 GCGGTGGTAA CGGCGCAGTG GCGGTTTTCA TGGCTTGTTA TGACTGTTTT TTTGTACAGC
     2161 CTATGCCTCG GGCATCCAAG CAGCAAGCGC GTTACGCCGT GGGTCGATGT TTGATGTTAT
     2221 GGAGCAGCAA CGATGTTACG CAGCAGCAAC GATGTTACGC AGCAGGGCAG TCGCCCTAAA
     2281 ACAAAGTTAG GTGGCTCAAG TATGGGCATC ATTCGCACAT GTAGGCTCGG CCCTGACCAA
     2341 GTCAAATCCA TGCGGGCTGC TCTTGATCTT TTCGGTCGTG AGTTCGGAGA CGTAGCCACC
     2401 TACTCCCAAC ATCAGCCGGA CTCCGATTAC CTCGGGAACT TGCTCCGTAG TAAGACATTC
     2461 ATCGCGCTTG CTGCCTTCGA CCAAGAAGCG GTTGTTGGCG CTCTCGCGGC TTACGTTCTG
     2521 CCCAAGTTTG AGCAGCCGCG TAGTGAGATC TATATCTATG ATCTCGCAGT CTCCGGAGAG
     2581 CACCGGAGGC AGGGCATTGC CACCGCGCTC ATCAATCTCC TCAAGCATGA GGCCAACGCG
     2641 CTTGGTGCTT ATGTGATCTA CGTGCAAGCA GATTACGGTG ACGATCCCGC AGTGGCTCTC
     2701 TATACAAAGT TGGGCATACG GGAAGAAGTG ATGCACTTTG ATATCGACCC AAGTACCGCC
     2761 ACCTAACAAT TCGTTCAAGC CGAGATCGGC TTCCCGGCCG CGGAGTTGTT CGGTAAATTG
     2821 GACAACGGTC CGCGCGTTGT CCTTTTCCGC TGCATAACCC TGCTTCGGGG TCATTATAGC
     2881 GATTTTTTCG GTATATCCAT CCTTTTTCGC ACGATATACA GGATTTTGCC AAAGGGTTCG
     2941 TGTAGACTTT CCTTGGTGTA TCCAACGGCG TCAGCCGGGC AGGATAGGTG AAGTAGGCCC
     3001 ACCCGCGAGC GGGTGTTCCT TCT
//