LOCUS       Untitled_Sequence        3021 bp    DNA     circular SYN 28-FEB-2023
FEATURES             Location/Qualifiers
     primer_bind     329..345
                     /label="M13 fwd"
                     /color="#a020f0"
     primer_bind     243..259
                     /label="M13 rev"
                     /color="#a020f0"
     protein_bind    219..235
                     /label="lac operator"
                     /color="#31849b"
     protein_bind    2876..2897
                     /label="CAP binding site"
                     /color="#31849b"
     protein_bind    145..166
                     /label="CAP binding site"
                     /color="#31849b"
     promoter        918..923
                     /label="Pc promoter"
                     /color="#ffffff"
     promoter        205..211
                     /label="lac promoter"
                     /color="#ffffff"
     misc_feature    272..328
                     /label="MCS"
                     /color="#99ccff"
     terminator      2931..3017
                     /label="rrnB T1 terminator"
                     /color="#ffffff"
     terminator      770..864
                     /label="lambda t0 terminator"
                     /color="#ffffff"
     oriT            1856..1964
                     /label="oriT"
                     /color="#a6acb3"
     CDS             255..578
                     /label="lacZα"
                     /color="#993366"
     CDS             1112..1645
                     /label="GmR"
                     /color="#ccffcc"
     rep_origin      2000..2588
                     /label="ori"
                     /color="#ffff00"
     misc_feature    87..90
                     /label="1"
                     /color="#99cc00"
     misc_feature    83..86
                     /label="E"
                     /color="#00ffff"
     misc_feature    675..678
                     /label="7"
                     /color="#99cc00"
     misc_feature    679..682
                     /label="F"
                     /color="#00ffff"
ORIGIN      
        1 TTAATTAAAG CGGATAACAA TTTCACACAG GAGGCCGCCT AGGCCGCGGC CGCGCGAATT
       61 CGAGCTCGGT ACCCgGGTCT CaAGGTTGCC ttGTCTTCac gacaggtttg ccgactggAA
      121 AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC
      181 TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA
      241 CACAGGAAAC AGCTATGACC ATGATTACGC CAAGCTTGCA TGCCTGCAGG TCGACTCTAG
      301 AGGATCCCCG GGTACCGAGC TCGAATTCAC TGGCCGTCGT TTTACAACGT CGTGACTGGG
      361 AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA TCCCCCTTTC GCCAGCTGGC
      421 GTAATAGCGA AGAGGCCCGC ACCGATCGCC CTTCCCAACA GTTGCGCAGC CTGAATGGCG
      481 AATGGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT
      541 GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGCCC CGACACCCGC
      601 CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG
      661 CTGTGAGAAG ACAAGAGCTT CGTGAGACCG GGGATCCTCT AGAGTCGACC TGCAGGCATG
      721 CAAGCTTGCG GCCGCGTCGT GACTGGGAAA ACCCTGGCGA CTAGTCTTGG ACTCCTGTTG
      781 ATAGATCCAG TAATGACCTC AGAACTCCAT CTGGATTTGT TCAGAACGCT CGGTTGCCGC
      841 CGGGCGTTTT TTATTGGTGA GAATCCAGGG GTCCCCAATA ATTACGATTT AAATTTGACA
      901 TAAGCCTGTT CGGTTCGTAA ACTGTAATGC AAGTAGCGTA TGCGCTCACG CAACTGGTCC
      961 AGAACCTTGA CCGAACGCAG CGGTGGTAAC GGCGCAGTGG CGGTTTTCAT GGCTTGTTAT
     1021 GACTGTTTTT TTGTACAGCC TATGCCTCGG GCATCCAAGC AGCAAGCGCG TTACGCCGTG
     1081 GGTCGATGTT TGATGTTATG GAGCAGCAAC GATGTTACGC AGCAGCAACG ATGTTACGCA
     1141 GCAGGGCAGT CGCCCTAAAA CAAAGTTAGG TGGCTCAAGT ATGGGCATCA TTCGCACATG
     1201 TAGGCTCGGC CCTGACCAAG TCAAATCCAT GCGGGCTGCT CTTGATCTTT TCGGTCGTGA
     1261 GTTCGGAGAC GTAGCCACCT ACTCCCAACA TCAGCCGGAC TCCGATTACC TCGGGAACTT
     1321 GCTCCGTAGT AAGACATTCA TCGCGCTTGC TGCCTTCGAC CAAGAAGCGG TTGTTGGCGC
     1381 TCTCGCGGCT TACGTTCTGC CCAAGTTTGA GCAGCCGCGT AGTGAGATCT ATATCTATGA
     1441 TCTCGCAGTC TCCGGAGAGC ACCGGAGGCA GGGCATTGCC ACCGCGCTCA TCAATCTCCT
     1501 CAAGCATGAG GCCAACGCGC TTGGTGCTTA TGTGATCTAC GTGCAAGCAG ATTACGGTGA
     1561 CGATCCCGCA GTGGCTCTCT ATACAAAGTT GGGCATACGG GAAGAAGTGA TGCACTTTGA
     1621 TATCGACCCA AGTACCGCCA CCTAACAATT CGTTCAAGCC GAGATCGGCT TCCCGGCCGC
     1681 GGAGTTGTTC GGTAAATTGG ACAACGGTCC GCGCGTTGTC CTTTTCCGCT GCATAACCCT
     1741 GCTTCGGGGT CATTATAGCG ATTTTTTCGG TATATCCATC CTTTTTCGCA CGATATACAG
     1801 GATTTTGCCA AAGGGTTCGT GTAGACTTTC CTTGGTGTAT CCAACGGCGT CAGCCGGGCA
     1861 GGATAGGTGA AGTAGGCCCA CCCGCGAGCG GGTGTTCCTT CTTCACTGTC CCTTATTCGC
     1921 ACCTGGCGGT GCTCAACGGG AATCCTGCTC TGCGAGGCTG GCCGTAGGCC GGCCccgtag
     1981 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     2041 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     2101 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     2161 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     2221 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     2281 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     2341 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctttgagaaa
     2401 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     2461 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     2521 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     2581 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     2641 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     2701 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     2761 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     2821 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2881 tgagttagct cactcattag gcaGGCGCGC CCAGCTGTCT AGGGCGGCGG ATTTGTCCTA
     2941 CTCAGGAGAG CGTTCACCGA CAAACAACAG ATAAAACGAA AGGCCCAGTC TTTCGACTGA
     3001 GCCTTTCGTT TTATTTGATG C   
//