LOCUS       Untitled_Sequence        3749 bp    DNA     circular SYN 29-JUL-2022
FEATURES             Location/Qualifiers
     primer_bind     339..355
                     /label="M13 fwd"
                     /color="#a020f0"
     primer_bind     253..269
                     /label="M13 rev"
                     /color="#a020f0"
     protein_bind    229..245
                     /label="lac operator"
                     /color="#31849b"
     protein_bind    155..176
                     /label="CAP binding site"
                     /color="#31849b"
     promoter        215..221
                     /label="lac promoter"
                     /color="#ffffff"
     misc_feature    282..338
                     /label="MCS"
                     /color="#99ccff"
     terminator      3657..3743
                     /label="rrnB T1 terminator"
                     /color="#ffffff"
     terminator      781..875
                     /label="lambda t0 terminator"
                     /color="#ffffff"
     oriT            1989..2097
                     /label="oriT"
                     /color="#a6acb3"
     CDS             265..588
                     /label="lacZα"
                     /color="#993366"
     CDS             2108..2770
                     /label="pBBR1 Rep"
                     /color="#993366"
     rep_origin      2771..3542
                     /label="pBBR1 oriV"
                     /color="#ffff00"
     CDS             1007..1822
                     /label="KanR"
                     /color="#ccffcc"
ORIGIN      
        1 TTAATTAAAG CGGATAACAA TTTCACACAG GAGGCCGCCT AGGCCGCGGC CGCGCGAATT
       61 CGAGCTCGGT ACCCGGGAAG ACAATGCCAG CGTGAGACCG CAGCAGGCAC GACAGGTTTG
      121 CCGACTGGAA AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG
      181 CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT
      241 AACAATTTCA CACAGGAAAC AGCTATGACC ATGATTACGC CAAGCTTGCA TGCCTGCAGG
      301 TCGACTCTAG AGGATCCCCG GGTACCGAGC TCGAATTCAC TGGCCGTCGT TTTACAACGT
      361 CGTGACTGGG AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA TCCCCCTTTC
      421 GCCAGCTGGC GTAATAGCGA AGAGGCCCGC ACCGATCGCC CTTCCCAACA GTTGCGCAGC
      481 CTGAATGGCG AATGGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA
      541 CACCGCATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGCCC
      601 CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT
      661 TACAGACAAG CTGTGACGGT CTCACTCCGC AATTGTCTTC GGGGATCCTC TAGAGTCGAC
      721 CTGCAGGCAT GCAAGCTTGC GGCCGCGTCG TGACTGGGAA AACCCTGGCG ACTAGTCTTG
      781 GACTCCTGTT GATAGATCCA GTAATGACCT CAGAACTCCA TCTGGATTTG TTCAGAACGC
      841 TCGGTTGCCG CCGGGCGTTT TTTATTGGTG AGAATCCAGG GGTCCCCAAT AATTACGATT
      901 TAAATTTGTG TCTCAAAATC TCTGATGTTA CATTGCACAA GATAAAAATA TATCATCATG
      961 AACAATAAAA CTGTCTGCTT ACATAAACAG TAATACAAGG GGTGTTATGA GCCATATTCA
     1021 GCGTGAAACG AGCTGTAGCC GTCCGCGTCT GAACAGCAAC ATGGATGCGG ATCTGTATGG
     1081 CTATAAATGG GCGCGTGATA ACGTGGGTCA GAGCGGCGCG ACCATTTATC GTCTGTATGG
     1141 CAAACCGGAT GCGCCGGAAC TGTTTCTGAA ACATGGCAAA GGCAGCGTGG CGAACGATGT
     1201 GACCGATGAA ATGGTGCGTC TGAACTGGCT GACCGAATTT ATGCCGCTGC CGACCATTAA
     1261 ACATTTTATT CGCACCCCGG ATGATGCGTG GCTGCTGACC ACCGCGATTC CGGGCAAAAC
     1321 CGCGTTTCAG GTGCTGGAAG AATATCCGGA TAGCGGCGAA AACATTGTGG ATGCGCTGGC
     1381 CGTGTTTCTG CGTCGTCTGC ATAGCATTCC GGTGTGCAAC TGCCCGTTTA ACAGCGATCG
     1441 TGTGTTTCGT CTGGCCCAGG CGCAGAGCCG TATGAACAAC GGCCTGGTGG ATGCGAGCGA
     1501 TTTTGATGAT GAACGTAACG GCTGGCCGGT GGAACAGGTG TGGAAAGAAA TGCATAAACT
     1561 GCTGCCGTTT AGCCCGGATA GCGTGGTGAC CCACGGCGAT TTTAGCCTGG ATAACCTGAT
     1621 TTTCGATGAA GGCAAACTGA TTGGCTGCAT TGATGTGGGC CGTGTGGGCA TTGCGGATCG
     1681 TTATCAGGAT CTGGCCATTC TGTGGAACTG CCTGGGCGAA TTTAGCCCGA GCCTGCAAAA
     1741 ACGTCTGTTT CAGAAATATG GCATTGATAA TCCGGATATG AACAAACTGC AATTTCATCT
     1801 GATGCTGGAT GAATTTTTCT AATAATTAAT TGGACCGCGG TCCGCGCGTT GTCCTTTTCC
     1861 GCTGCATAAC CCTGCTTCGG GGTCATTATA GCGATTTTTT CGGTATATCC ATCCTTTTTC
     1921 GCACGATATA CAGGATTTTG CCAAAGGGTT CGTGTAGACT TTCCTTGGTG TATCCAACGG
     1981 CGTCAGCCGG GCAGGATAGG TGAAGTAGGC CCACCCGCGA GCGGGTGTTC CTTCTTCACT
     2041 GTCCCTTATT CGCACCTGGC GGTGCTCAAC GGGAATCCTG CTCTGCGAGG CTGGCCGTAG
     2101 GCCGGCCCTA CCGGCGCGGC AGCGTTACCC GTGTCGGCGG CTCCAACGGC TCGCCATCGT
     2161 CCAGAAAACA CGGCTCATCG GGCATCGGCA GGCGCTGCTG CCCGCGCCGT TCCCATTCCT
     2221 CCGTTTCGGT CAAGGCTGGC AGGTCTGGTT CCATGCCCGG AATGCCGGGC TGGCTGGGCG
     2281 GCTCCTCGCC GGGGCCGGTC GGTAGTTGCT GCTCGCCCGG ATACAGGGTC GGGATGCGGC
     2341 GCAGGTCGCC ATGCCCCAAC AGCGATTCGT CCTGGTCGTC GTGATCAACC ACCACGGCGG
     2401 CACTGAACAC CGACAGGCGC AACTGGTCGC GGGGCTGGCC CCACGCCACG CGGTCATTGA
     2461 CCACGTAGGC CGACACGGTG CCGGGGCCGT TGAGCTTCAC GACGGAGATC CAGCGCTCGG
     2521 CCACCAAGTC CTTGACTGCG TATTGGACCG TCCGCAAAGA ACGTCCGATG AGCTTGGAAA
     2581 GTGTTTTCTG GCTGACCACC ACGGCGTTCT GGTGGCCCAT CTGCGCCACG AGGTGATGCA
     2641 GCAGCATTGC CGCCGTGGGT TTCCTCGCAA TAAGCCCGGC CCACGCCTCA TGCGCTTTGC
     2701 GTTCCGTTTG CACCCAGTGA CCGGGCTTGT TCTTGGCTTG AATGCCGATT TCTCTGGACT
     2761 GCGTGGCCAT GCTTATCTCC ATGCGGTAGG GGTGCCGCAC GGTTGCGGCA CCATGCGCAA
     2821 TCAGCTGCAA CTTTTCGGCA GCGCGACAAC AATTATGCGT TGCGTAAAAG TGGCAGTCAA
     2881 TTACAGATTT TCTTTAACCT ACGCAATGAG CTATTGCGGG GGGTGCCGCA ATGAGCTGTT
     2941 GCGTACCCCC CTTTTTTAAG TTGTTGATTT TTAAGTCTTT CGCATTTCGC CCTATATCTA
     3001 GTTCTTTGGT GCCCAAAGAA GGGCACCCCT GCGGGGTTCC CCCACGCCTT CGGCGCGGCT
     3061 CCCCCTCCGG CAAAAAGTGG CCCCTCCGGG GCTTGTTGAT CGACTGCGCG GCCTTCGGCC
     3121 TTGCCCAAGG TGGCGCTGCC CCCTTGGAAC CCCCGCACTC GCCGCCGTGA GGCTCGGGGG
     3181 GCAGGCGGGC GGGCTTCGCC CTTCGACTGC CCCCACTCGC ATAGGCTTGG GTCGTTCCAG
     3241 GCGCGTCAAG GCCAAGCCGC TGCGCGGTCG CTGCGCGAGC CTTGACCCGC CTTCCACTTG
     3301 GTGTCCAACC GGCAAGCGAA GCGCGCAGGC CGCAGGCCGG AGGCTTTTCC CCAGAGAAAA
     3361 TTAAAAAAAT TGATGGGGCA AGGCCGCAGG CCGCGCAGTT GGAGCCGGTG GGTATGTGGT
     3421 CGAAGGCTGG GTAGCCGGTG GGCAATCCCT GTGGTCAAGC TCGTGGGCAG GCGCAGCCTG
     3481 TCCATCAGCT TGTCCAGCAG GGTTGTCCAC GGGCCGAGCG AAGCGAGCCA GCCGGTGGCC
     3541 GCTCGCGGCC ATCGTCCACA TATCCACGGG CTGGCAAGGG AGCGCAGCGA CCGCGCAGGG
     3601 CGAAGCCCGG AGAGCAAGCC CGTAGGGGGG GCGCGCCCAG CTGTCTAGGG CGGCGGATTT
     3661 GTCCTACTCA GGAGAGCGTT CACCGACAAA CAACAGATAA AACGAAAGGC CCAGTCTTTC
     3721 GACTGAGCCT TTCGTTTTAT TTGATGCCT   
//
