LOCUS       Untitled_Sequence        3156 bp    DNA     circular SYN 29-JUL-2022
FEATURES             Location/Qualifiers
     primer_bind     339..355
                     /label="M13 fwd"
                     /color="#a020f0"
     primer_bind     253..269
                     /label="M13 rev"
                     /color="#a020f0"
     protein_bind    229..245
                     /label="lac operator"
                     /color="#31849b"
     protein_bind    3009..3030
                     /label="CAP binding site"
                     /color="#31849b"
     protein_bind    155..176
                     /label="CAP binding site"
                     /color="#31849b"
     promoter        215..221
                     /label="lac promoter"
                     /color="#ffffff"
     misc_feature    282..338
                     /label="MCS"
                     /color="#99ccff"
     terminator      3064..3150
                     /label="rrnB T1 terminator"
                     /color="#ffffff"
     terminator      781..875
                     /label="lambda t0 terminator"
                     /color="#ffffff"
     oriT            1989..2097
                     /label="oriT"
                     /color="#a6acb3"
     CDS             265..588
                     /label="lacZα"
                     /color="#993366"
     rep_origin      2133..2721
                     /label="ori"
                     /color="#ffff00"
     CDS             1007..1822
                     /label="KanR"
                     /color="#ccffcc"
ORIGIN      
        1 TTAATTAAAG CGGATAACAA TTTCACACAG GAGGCCGCCT AGGCCGCGGC CGCGCGAATT
       61 CGAGCTCGGT ACCCGGGAAG ACAATGCCAG CGTGAGACCG CAGCAGGCAC GACAGGTTTG
      121 CCGACTGGAA AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG
      181 CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT
      241 AACAATTTCA CACAGGAAAC AGCTATGACC ATGATTACGC CAAGCTTGCA TGCCTGCAGG
      301 TCGACTCTAG AGGATCCCCG GGTACCGAGC TCGAATTCAC TGGCCGTCGT TTTACAACGT
      361 CGTGACTGGG AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA TCCCCCTTTC
      421 GCCAGCTGGC GTAATAGCGA AGAGGCCCGC ACCGATCGCC CTTCCCAACA GTTGCGCAGC
      481 CTGAATGGCG AATGGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA
      541 CACCGCATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGCCC
      601 CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT
      661 TACAGACAAG CTGTGACGGT CTCACTCCGC AATTGTCTTC GGGGATCCTC TAGAGTCGAC
      721 CTGCAGGCAT GCAAGCTTGC GGCCGCGTCG TGACTGGGAA AACCCTGGCG ACTAGTCTTG
      781 GACTCCTGTT GATAGATCCA GTAATGACCT CAGAACTCCA TCTGGATTTG TTCAGAACGC
      841 TCGGTTGCCG CCGGGCGTTT TTTATTGGTG AGAATCCAGG GGTCCCCAAT AATTACGATT
      901 TAAATTTGTG TCTCAAAATC TCTGATGTTA CATTGCACAA GATAAAAATA TATCATCATG
      961 AACAATAAAA CTGTCTGCTT ACATAAACAG TAATACAAGG GGTGTTATGA GCCATATTCA
     1021 GCGTGAAACG AGCTGTAGCC GTCCGCGTCT GAACAGCAAC ATGGATGCGG ATCTGTATGG
     1081 CTATAAATGG GCGCGTGATA ACGTGGGTCA GAGCGGCGCG ACCATTTATC GTCTGTATGG
     1141 CAAACCGGAT GCGCCGGAAC TGTTTCTGAA ACATGGCAAA GGCAGCGTGG CGAACGATGT
     1201 GACCGATGAA ATGGTGCGTC TGAACTGGCT GACCGAATTT ATGCCGCTGC CGACCATTAA
     1261 ACATTTTATT CGCACCCCGG ATGATGCGTG GCTGCTGACC ACCGCGATTC CGGGCAAAAC
     1321 CGCGTTTCAG GTGCTGGAAG AATATCCGGA TAGCGGCGAA AACATTGTGG ATGCGCTGGC
     1381 CGTGTTTCTG CGTCGTCTGC ATAGCATTCC GGTGTGCAAC TGCCCGTTTA ACAGCGATCG
     1441 TGTGTTTCGT CTGGCCCAGG CGCAGAGCCG TATGAACAAC GGCCTGGTGG ATGCGAGCGA
     1501 TTTTGATGAT GAACGTAACG GCTGGCCGGT GGAACAGGTG TGGAAAGAAA TGCATAAACT
     1561 GCTGCCGTTT AGCCCGGATA GCGTGGTGAC CCACGGCGAT TTTAGCCTGG ATAACCTGAT
     1621 TTTCGATGAA GGCAAACTGA TTGGCTGCAT TGATGTGGGC CGTGTGGGCA TTGCGGATCG
     1681 TTATCAGGAT CTGGCCATTC TGTGGAACTG CCTGGGCGAA TTTAGCCCGA GCCTGCAAAA
     1741 ACGTCTGTTT CAGAAATATG GCATTGATAA TCCGGATATG AACAAACTGC AATTTCATCT
     1801 GATGCTGGAT GAATTTTTCT AATAATTAAT TGGACCGCGG TCCGCGCGTT GTCCTTTTCC
     1861 GCTGCATAAC CCTGCTTCGG GGTCATTATA GCGATTTTTT CGGTATATCC ATCCTTTTTC
     1921 GCACGATATA CAGGATTTTG CCAAAGGGTT CGTGTAGACT TTCCTTGGTG TATCCAACGG
     1981 CGTCAGCCGG GCAGGATAGG TGAAGTAGGC CCACCCGCGA GCGGGTGTTC CTTCTTCACT
     2041 GTCCCTTATT CGCACCTGGC GGTGCTCAAC GGGAATCCTG CTCTGCGAGG CTGGCCGTAG
     2101 GCCGGCCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC
     2161 TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG
     2221 CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTT
     2281 CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC
     2341 CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC
     2401 GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT
     2461 TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT
     2521 GAGCTTTGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC
     2581 GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT
     2641 TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA
     2701 GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT
     2761 TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT
     2821 ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG
     2881 TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA ATACGCAAAC CGCCTCTCCC CGCGCGTTGG
     2941 CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT GGAAAGCGGG CAGTGAGCGC
     3001 AACGCAATTA ATGTGAGTTA GCTCACTCAT TAGGCAGGCG CGCCCAGCTG TCTAGGGCGG
     3061 CGGATTTGTC CTACTCAGGA GAGCGTTCAC CGACAAACAA CAGATAAAAC GAAAGGCCCA
     3121 GTCTTTCGAC TGAGCCTTTC GTTTTATTTG ATGCCT  
//
