LOCUS       Untitled_Sequence        4316 bp    DNA     circular SYN 28-FEB-2023
FEATURES             Location/Qualifiers
     CDS             2025..3173
                     /label="trfA"
                     /color="#993366"
     CDS             1112..1645
                     /label="GmR"
                     /color="#ccffcc"
     rep_origin      3566..4097
                     /label="oriV"
                     /color="#ffff00"
     oriT            1856..1965
                     /label="oriT"
                     /color="#a6acb3"
     terminator      770..864
                     /label="lambda t0 terminator"
                     /color="#ffffff"
     terminator      4224..4310
                     /label="rrnB T1 terminator"
                     /color="#ffffff"
     promoter        918..923
                     /label="Pc promoter"
                     /color="#ffffff"
     primer_bind     329..345
                     /label="M13 fwd"
                     /color="#a020f0"
     primer_bind     243..259
                     /label="M13 rev"
                     /color="#a020f0"
     protein_bind    219..235
                     /label="lac operator"
                     /color="#31849b"
     protein_bind    145..166
                     /label="CAP binding site"
                     /color="#31849b"
     promoter        205..211
                     /label="lac promoter"
                     /color="#ffffff"
     misc_feature    272..328
                     /label="MCS"
                     /color="#99ccff"
     CDS             255..578
                     /label="lacZα"
                     /color="#993366"
     misc_feature    87..90
                     /label="1"
                     /color="#99cc00"
     misc_feature    83..86
                     /label="D"
                     /color="#00ffff"
     misc_feature    675..678
                     /label="6"
                     /color="#99cc00"
     misc_feature    679..682
                     /label="E"
                     /color="#00ffff"
ORIGIN      
        1 TTAATTAAAG CGGATAACAA TTTCACACAG GAGGCCGCCT AGGCCGCGGC CGCGCGAATT
       61 CGAGCTCGGT ACCCGGGTCT CAAATGTGCC TTGTCTTCAC GACAGGTTTG CCGACTGGAA
      121 AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC
      181 TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA
      241 CACAGGAAAC AGCTATGACC ATGATTACGC CAAGCTTGCA TGCCTGCAGG TCGACTCTAG
      301 AGGATCCCCG GGTACCGAGC TCGAATTCAC TGGCCGTCGT TTTACAACGT CGTGACTGGG
      361 AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA TCCCCCTTTC GCCAGCTGGC
      421 GTAATAGCGA AGAGGCCCGC ACCGATCGCC CTTCCCAACA GTTGCGCAGC CTGAATGGCG
      481 AATGGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT
      541 GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGCCC CGACACCCGC
      601 CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG
      661 CTGTGAGAAG ACAATGTGAG GTTGAGACCG GGGATCCTCT AGAGTCGACC TGCAGGCATG
      721 CAAGCTTGCG GCCGCGTCGT GACTGGGAAA ACCCTGGCGA CTAGTCTTGG ACTCCTGTTG
      781 ATAGATCCAG TAATGACCTC AGAACTCCAT CTGGATTTGT TCAGAACGCT CGGTTGCCGC
      841 CGGGCGTTTT TTATTGGTGA GAATCCAGGG GTCCCCAATA ATTACGATTT AAATTTGACA
      901 TAAGCCTGTT CGGTTCGTAA ACTGTAATGC AAGTAGCGTA TGCGCTCACG CAACTGGTCC
      961 AGAACCTTGA CCGAACGCAG CGGTGGTAAC GGCGCAGTGG CGGTTTTCAT GGCTTGTTAT
     1021 GACTGTTTTT TTGTACAGCC TATGCCTCGG GCATCCAAGC AGCAAGCGCG TTACGCCGTG
     1081 GGTCGATGTT TGATGTTATG GAGCAGCAAC GATGTTACGC AGCAGCAACG ATGTTACGCA
     1141 GCAGGGCAGT CGCCCTAAAA CAAAGTTAGG TGGCTCAAGT ATGGGCATCA TTCGCACATG
     1201 TAGGCTCGGC CCTGACCAAG TCAAATCCAT GCGGGCTGCT CTTGATCTTT TCGGTCGTGA
     1261 GTTCGGAGAC GTAGCCACCT ACTCCCAACA TCAGCCGGAC TCCGATTACC TCGGGAACTT
     1321 GCTCCGTAGT AAGACATTCA TCGCGCTTGC TGCCTTCGAC CAAGAAGCGG TTGTTGGCGC
     1381 TCTCGCGGCT TACGTTCTGC CCAAGTTTGA GCAGCCGCGT AGTGAGATCT ATATCTATGA
     1441 TCTCGCAGTC TCCGGAGAGC ACCGGAGGCA GGGCATTGCC ACCGCGCTCA TCAATCTCCT
     1501 CAAGCATGAG GCCAACGCGC TTGGTGCTTA TGTGATCTAC GTGCAAGCAG ATTACGGTGA
     1561 CGATCCCGCA GTGGCTCTCT ATACAAAGTT GGGCATACGG GAAGAAGTGA TGCACTTTGA
     1621 TATCGACCCA AGTACCGCCA CCTAACAATT CGTTCAAGCC GAGATCGGCT TCCCGGCCGC
     1681 GGAGTTGTTC GGTAAATTGG ACAACGGTCC GCGCGTTGTC CTTTTCCGCT GCATAACCCT
     1741 GCTTCGGGGT CATTATAGCG ATTTTTTCGG TATATCCATC CTTTTTCGCA CGATATACAG
     1801 GATTTTGCCA AAGGGTTCGT GTAGACTTTC CTTGGTGTAT CCAACGGCGT CAGCCGGGCA
     1861 GGATAGGTGA AGTAGGCCCA CCCGCGAGCG GGTGTTCCTT CTTCACTGTC CCTTATTCGC
     1921 ACCTGGCGGT GCTCAACGGG AATCCTGCTC TGCGAGGCTG GCCGTAGGCC GGCCGCGATG
     1981 CAGGTGGCTG CTGAACCCCC AGCCGGAACT GACCCCACAA GGCCCTAGCG TTTGCAATGC
     2041 ACCAGGTCAT CATTGACCCA GGCGTGTTCC ACCAGGCCGC TGCCTCGCAA CTCTTCGCAG
     2101 GCTTCGCCGA CCTGCTCGCG CCACTTCTTC ACGCGGGTGG AATCCGATCC GCACATGAGG
     2161 CGGAAGGTTT CCAGCTTGAG CGGGTACGGC TCCCGGTGCG AGCTGAAATA GTCGAACATC
     2221 CGTCGGGCCG TCGGCGACAG CTTGCGGTAC TTCTCCCATA TGAATTTCGT GTAGTGGTCG
     2281 CCAGCAAACA GCACGACGAT TTCCTCGTCG ATCAGGACCT GGCAACGGGA CGTTTTCTTG
     2341 CCACGGTCCA GGACGCGGAA GCGGTGCAGC AGCGACACCG ATTCCAGGTG CCCAACGCGG
     2401 TCGGACGTGA AGCCCATCGC CGTCGCCTGT AGGCGCGACA GGCATTCCTC GGCCTTCGTG
     2461 TAATACCGGC CATTGATCGA CCAGCCCAGG TCCTGGCAAA GCTCGTAGAA CGTGAAGGTG
     2521 ATCGGCTCGC CGATAGGGGT GCGCTTCGCG TACTCCAACA CCTGCTGCCA CACCAGTTCG
     2581 TCATCGTCGG CCCGCAGCTC GACGCCGGTG TAGGTGATCT TCACGTCCTT GTTGACGTGG
     2641 AAAATGACCT TGTTTTGCAG CGCCTCGCGC GGGATTTTCT TGTTGCGCGT GGTGAACAGG
     2701 GCAGAGCGGG CCGTGTCGTT TGGCATCGCT CGCATCGTGT CCGGCCACGG CGCAATATCG
     2761 AACAAGGAAA GCTGCATTTC CTTGATCTGC TGCTTCGTGT GTTTCAGCAA CGCGGCCTGC
     2821 TTGGCTTCGC TGACCTGTTT TGCCAGGTCC TCGCCGGCGG TTTTTCGCTT CTTGGTCGTC
     2881 ATAGTTCCTC GCGTGTCGAT GGTCATCGAC TTCGCCAAAC CTGCCGCCTC CTGTTCGAGA
     2941 CGACGCGAAC GCTCCACGGC GGCCGATGGC GCGGGCAGGG CAGGGGGAGC CAGTTGCACG
     3001 CTGTCGCGCT CGATCTTGGC CGTAGCTTGC TGGACTATCG AGCCGACGGA CTGGAAGGTT
     3061 TCGCGGGGCG CACGCATGAC GGTGCGGCTT GCGATGGTTT CGGCATCCTC GGCGGAAAAC
     3121 CCCGCGTCGA TCAGTTCTTG CCTGTATGCC TTCCGGTCAA ACGTCCGATT CATTCACCCT
     3181 CCTTGCGGGA TTGCCCCGGA ATTAATTCCC CGGATCGATC CGTCGATCTT GATCCCCTGC
     3241 GCCATCAGAT CCTTGGCGGC AAGAAAGCCA TCCAGTTTAC TTTGCAGGGC TTCCCAACCT
     3301 TACCAGAGGG CGCCCCAGCT GGCAATTCCG GTTCGCTTGC TGTCCATAAA ACCGCCCAGT
     3361 CTAGCTATCG CCATGTAAGC CCACTGCAAG CTACCTGCTT TCTCTTTGCG CTTGCGTTTT
     3421 CCCTTGTCCA GATAGCCCAG TAGCTGACAT TCATCCGGGG TCAGCACCGT TTCTGCGGAC
     3481 TGGCTTTCTA CGTGGCTGCC ATTTTTGGGG TGAGGCCGTT CGCGGCCGAG GGGCGCAGCC
     3541 CCTGGGGGGA TGGGAGGCCC GCGTTAGCGG GCCGGGAGGG TTCGAGAAGG GGGGGCACCC
     3601 CCCTTCGGCG TGCGCGGTCA CGCGCACAGG GCGCAGCCCT GGTTAAAAAC AAGGTTTATA
     3661 AATATTGGTT TAAAAGCAGG TTAAAAGACA GGTTAGCGGT GGCCGAAAAA CGGGCGGAAA
     3721 CCCTTGCAAA TGCTGGATTT TCTGCCTGTG GACAGCCCCT CAAATGTCAA TAGGTGCGCC
     3781 CCTCATCTGT CAGCACTCTG CCCCTCAAGT GTCAAGGATC GCGCCCCTCA TCTGTCAGTA
     3841 GTCGCGCCCC TCAAGTGTCA ATACCGCAGG GCACTTATCC CCAGGCTTGT CCACATCATC
     3901 TGTGGGAAAC TCGCGTAAAA TCAGGCGTTT TCGCCGATTT GCGAGGCTGG CCAGCTCCAC
     3961 GTCGCCGGCC GAAATCGAGC CTGCCCCTCA TCTGTCAACG CCGCGCCGGG TGAGTCGGCC
     4021 CCTCAAGTGT CAACGTCCGC CCCTCATCTG TCAGTGAGGG CCAAGTTTTC CGCGAGGTAT
     4081 CCACAACGCC GGCGGCCCTA CATGGCTCTG CTGTAGTGAG TGGGTTGCGC TCCGGCAGCG
     4141 GTCCTGATCC CCCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGCG
     4201 CGCCCAGCTG TCTAGGGCGG CGGATTTGTC CTACTCAGGA GAGCGTTCAC CGACAAACAA
     4261 CAGATAAAAC GAAAGGCCCA GTCTTTCGAC TGAGCCTTTC GTTTTATTTG ATGCCT
//
