LOCUS       Untitled_Sequence        3751 bp    DNA     circular SYN 28-FEB-2023
FEATURES             Location/Qualifiers
     primer_bind     339..355
                     /label="M13 fwd"
                     /color="#a020f0"
     primer_bind     253..269
                     /label="M13 rev"
                     /color="#a020f0"
     protein_bind    229..245
                     /label="lac operator"
                     /color="#31849b"
     protein_bind    155..176
                     /label="CAP binding site"
                     /color="#31849b"
     promoter        215..221
                     /label="lac promoter"
                     /color="#ffffff"
     misc_feature    282..338
                     /label="MCS"
                     /color="#99ccff"
     terminator      3659..3745
                     /label="rrnB T1 terminator"
                     /color="#ffffff"
     terminator      783..877
                     /label="lambda t0 terminator"
                     /color="#ffffff"
     oriT            1991..2099
                     /label="oriT"
                     /color="#a6acb3"
     CDS             265..588
                     /label="lacZα"
                     /color="#993366"
     CDS             2110..2772
                     /label="pBBR1 Rep"
                     /color="#993366"
     rep_origin      2773..3544
                     /label="pBBR1 oriV"
                     /color="#ffff00"
     CDS             1009..1824
                     /label="KanR"
                     /color="#ccffcc"
     misc_feature    89..92
                     /label="A"
                     /color="#00ffff"
     misc_feature    85..88
                     /label="3"
                     /color="#99cc00"
     misc_feature    685..688
                     /label="E"
                     /color="#00ffff"
     misc_feature    689..692
                     /label="4"
                     /color="#99cc00"
ORIGIN      
        1 TTAATTAAAG CGGATAACAA TTTCACACAG GAGGCCGCCT AGGCCGCGGC CGCGCGAATT
       61 CGAGCTCGGT ACCCGGGAAG ACAAACTAGG AGTGAGACCG CAGCTGGCAC GACAGGTTTG
      121 CCGACTGGAA AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG
      181 CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT
      241 AACAATTTCA CACAGGAAAC AGCTATGACC ATGATTACGC CAAGCTTGCA TGCCTGCAGG
      301 TCGACTCTAG AGGATCCCCG GGTACCGAGC TCGAATTCAC TGGCCGTCGT TTTACAACGT
      361 CGTGACTGGG AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA TCCCCCTTTC
      421 GCCAGCTGGC GTAATAGCGA AGAGGCCCGC ACCGATCGCC CTTCCCAACA GTTGCGCAGC
      481 CTGAATGGCG AATGGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA
      541 CACCGCATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGCCC
      601 CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT
      661 TACAGACAAG CTGTGACGGT CTCAAGGTTT ACTTGTCTTC GGGGGGATCC TCTAGAGTCG
      721 ACCTGCAGGC ATGCAAGCTT GCGGCCGCGT CGTGACTGGG AAAACCCTGG CGACTAGTCT
      781 TGGACTCCTG TTGATAGATC CAGTAATGAC CTCAGAACTC CATCTGGATT TGTTCAGAAC
      841 GCTCGGTTGC CGCCGGGCGT TTTTTATTGG TGAGAATCCA GGGGTCCCCA ATAATTACGA
      901 TTTAAATTTG TGTCTCAAAA TCTCTGATGT TACATTGCAC AAGATAAAAA TATATCATCA
      961 TGAACAATAA AACTGTCTGC TTACATAAAC AGTAATACAA GGGGTGTTAT GAGCCATATT
     1021 CAGCGTGAAA CGAGCTGTAG CCGTCCGCGT CTGAACAGCA ACATGGATGC GGATCTGTAT
     1081 GGCTATAAAT GGGCGCGTGA TAACGTGGGT CAGAGCGGCG CGACCATTTA TCGTCTGTAT
     1141 GGCAAACCGG ATGCGCCGGA ACTGTTTCTG AAACATGGCA AAGGCAGCGT GGCGAACGAT
     1201 GTGACCGATG AAATGGTGCG TCTGAACTGG CTGACCGAAT TTATGCCGCT GCCGACCATT
     1261 AAACATTTTA TTCGCACCCC GGATGATGCG TGGCTGCTGA CCACCGCGAT TCCGGGCAAA
     1321 ACCGCGTTTC AGGTGCTGGA AGAATATCCG GATAGCGGCG AAAACATTGT GGATGCGCTG
     1381 GCCGTGTTTC TGCGTCGTCT GCATAGCATT CCGGTGTGCA ACTGCCCGTT TAACAGCGAT
     1441 CGTGTGTTTC GTCTGGCCCA GGCGCAGAGC CGTATGAACA ACGGCCTGGT GGATGCGAGC
     1501 GATTTTGATG ATGAACGTAA CGGCTGGCCG GTGGAACAGG TGTGGAAAGA AATGCATAAA
     1561 CTGCTGCCGT TTAGCCCGGA TAGCGTGGTG ACCCACGGCG ATTTTAGCCT GGATAACCTG
     1621 ATTTTCGATG AAGGCAAACT GATTGGCTGC ATTGATGTGG GCCGTGTGGG CATTGCGGAT
     1681 CGTTATCAGG ATCTGGCCAT TCTGTGGAAC TGCCTGGGCG AATTTAGCCC GAGCCTGCAA
     1741 AAACGTCTGT TTCAGAAATA TGGCATTGAT AATCCGGATA TGAACAAACT GCAATTTCAT
     1801 CTGATGCTGG ATGAATTTTT CTAATAATTA ATTGGACCGC GGTCCGCGCG TTGTCCTTTT
     1861 CCGCTGCATA ACCCTGCTTC GGGGTCATTA TAGCGATTTT TTCGGTATAT CCATCCTTTT
     1921 TCGCACGATA TACAGGATTT TGCCAAAGGG TTCGTGTAGA CTTTCCTTGG TGTATCCAAC
     1981 GGCGTCAGCC GGGCAGGATA GGTGAAGTAG GCCCACCCGC GAGCGGGTGT TCCTTCTTCA
     2041 CTGTCCCTTA TTCGCACCTG GCGGTGCTCA ACGGGAATCC TGCTCTGCGA GGCTGGCCGT
     2101 AGGCCGGCCC TACCGGCGCG GCAGCGTTAC CCGTGTCGGC GGCTCCAACG GCTCGCCATC
     2161 GTCCAGAAAA CACGGCTCAT CGGGCATCGG CAGGCGCTGC TGCCCGCGCC GTTCCCATTC
     2221 CTCCGTTTCG GTCAAGGCTG GCAGGTCTGG TTCCATGCCC GGAATGCCGG GCTGGCTGGG
     2281 CGGCTCCTCG CCGGGGCCGG TCGGTAGTTG CTGCTCGCCC GGATACAGGG TCGGGATGCG
     2341 GCGCAGGTCG CCATGCCCCA ACAGCGATTC GTCCTGGTCG TCGTGATCAA CCACCACGGC
     2401 GGCACTGAAC ACCGACAGGC GCAACTGGTC GCGGGGCTGG CCCCACGCCA CGCGGTCATT
     2461 GACCACGTAG GCCGACACGG TGCCGGGGCC GTTGAGCTTC ACGACGGAGA TCCAGCGCTC
     2521 GGCCACCAAG TCCTTGACTG CGTATTGGAC CGTCCGCAAA GAACGTCCGA TGAGCTTGGA
     2581 AAGTGTTTTC TGGCTGACCA CCACGGCGTT CTGGTGGCCC ATCTGCGCCA CGAGGTGATG
     2641 CAGCAGCATT GCCGCCGTGG GTTTCCTCGC AATAAGCCCG GCCCACGCCT CATGCGCTTT
     2701 GCGTTCCGTT TGCACCCAGT GACCGGGCTT GTTCTTGGCT TGAATGCCGA TTTCTCTGGA
     2761 CTGCGTGGCC ATGCTTATCT CCATGCGGTA GGGGTGCCGC ACGGTTGCGG CACCATGCGC
     2821 AATCAGCTGC AACTTTTCGG CAGCGCGACA ACAATTATGC GTTGCGTAAA AGTGGCAGTC
     2881 AATTACAGAT TTTCTTTAAC CTACGCAATG AGCTATTGCG GGGGGTGCCG CAATGAGCTG
     2941 TTGCGTACCC CCCTTTTTTA AGTTGTTGAT TTTTAAGTCT TTCGCATTTC GCCCTATATC
     3001 TAGTTCTTTG GTGCCCAAAG AAGGGCACCC CTGCGGGGTT CCCCCACGCC TTCGGCGCGG
     3061 CTCCCCCTCC GGCAAAAAGT GGCCCCTCCG GGGCTTGTTG ATCGACTGCG CGGCCTTCGG
     3121 CCTTGCCCAA GGTGGCGCTG CCCCCTTGGA ACCCCCGCAC TCGCCGCCGT GAGGCTCGGG
     3181 GGGCAGGCGG GCGGGCTTCG CCCTTCGACT GCCCCCACTC GCATAGGCTT GGGTCGTTCC
     3241 AGGCGCGTCA AGGCCAAGCC GCTGCGCGGT CGCTGCGCGA GCCTTGACCC GCCTTCCACT
     3301 TGGTGTCCAA CCGGCAAGCG AAGCGCGCAG GCCGCAGGCC GGAGGCTTTT CCCCAGAGAA
     3361 AATTAAAAAA ATTGATGGGG CAAGGCCGCA GGCCGCGCAG TTGGAGCCGG TGGGTATGTG
     3421 GTCGAAGGCT GGGTAGCCGG TGGGCAATCC CTGTGGTCAA GCTCGTGGGC AGGCGCAGCC
     3481 TGTCCATCAG CTTGTCCAGC AGGGTTGTCC ACGGGCCGAG CGAAGCGAGC CAGCCGGTGG
     3541 CCGCTCGCGG CCATCGTCCA CATATCCACG GGCTGGCAAG GGAGCGCAGC GACCGCGCAG
     3601 GGCGAAGCCC GGAGAGCAAG CCCGTAGGGG GGGCGCGCCC AGCTGTCTAG GGCGGCGGAT
     3661 TTGTCCTACT CAGGAGAGCG TTCACCGACA AACAACAGAT AAAACGAAAG GCCCAGTCTT
     3721 TCGACTGAGC CTTTCGTTTT ATTTGATGCC T  
//
