Title: Streamlining and Reprogramming Biocatalysts
Authors: Vítor A.P. Martins dos Santos
Abstract:The pursuit of Synthetic Biology is both the design and fabrication of biological components and systems that do not exist in the natural world as well as the re-design and fabrication of already existing biological systems. I will provide a short overview on the current scope of Synthetic Biology for Industrial Biotechnology, on its challenges and perspectives, and will illustrate these through cases studies. Specifically, As an illustration, I will present a transnational project on Synthetic Biology that aims at constructing a functioning, streamlined bacterial cell devoid of most of its genome and endowed with a series of highly coordinated, newly assembled genetic circuits for the biotransformation of a range of chloroaromatics into high added value compounds and that include circuits for synchronized behaviour, noise minimisation and low-temperature biocatalysis. By achieving such constructs as a proof-of-principle, it is aimed at establishing a solid, rational framework for the streamlining and engineering of cells performing effectively and efficiently specific functions of biotechnological and medical interest. The added value comes from obtaining a streamlined bacterial factory, devoid of unnecessary gene complements and undesired cross-talk (and in which the internal wiring is well characterised), thereby enabling a higher degree of control and hence re-programming, by plugging-and-playing at will.
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