Golden Standard database


Type Code Fusion Sites Receptor Vector Name Description Other database entries Actions
Lv0 CDS c10 DGpSEVA182 RFP_DGmRFP1 fluorescent gene
Lv0 CDS c11 DGpSEVA182 GFP_DGGFPmut3 fluorescent gene
Lv0 CDS c215 EFpSEVA182 GST-HRV3C_EFGST HRV3C protease site
Linker l01 12pSEVA181 Linker_12Linker with 12 FS (BsaI)
Linker l02 23pSEVA181 Linker_23Linker with 23 FS (BsaI)
Linker l03 34pSEVA181 Linker_34Linker with 34 FS (BsaI)
Linker l04 45pSEVA181 Linker_45Linker with 45 FS (BsaI)
Linker l05 56pSEVA181 Linker_56Linker with 56 FS (BsaI)
Linker l06 67pSEVA182 Linker_67Linker with 67 FS (BsaI)
Linker l07 ABpSEVA182 Linker1_ABLinker with AB FS (BpiI)
Linker l08 BCpSEVA182 Linker_BCLinker with BC FS (BpiI)
Linker l09 CDpSEVA182 Linker_CDLinker with CD FS (BpiI)
Linker l10 DEpSEVA182 Linker_DELinker with DE FS (BpiI)
Linker l11 EFpSEVA182 Linker_EFLinker with EF FS (BpiI)
Linker l12 FGpSEVA182 Linker_FGLinker with FG FS (BpiI)
Linker l13 GHpSEVA182 Linker_GHLinker with GH FS (BpiI)
Linker l14 HIpSEVA182 Linker_HILinker with HI FS (BpiI)
Linker l15 GIpSEVA182 Linker1_GILinker with GI FS (BpiI)
Lv1 TU lv1_119 12pSEVA23g19[g1] RhaRS_12RhaRS Lv1 at position 12
Lv1 TU lv1_31 12pSEVA281 XylS_12XylS Lv1 at position 12
Lv1 TU lv1_33 12pSEVA23g19[g1] LacI_12LacI Lv1 at position 12
Lv0 Promoter p01 ABpSEVA182 Ptrc_ABPtrc promoter repressed by LacI
Lv0 Promoter p13 ABpSEVA182 J23100_ABJ23100 Anderson constitutive promoter
IGEM
Lv0 Promoter p14 ABpSEVA182 J23102_ABJ23102 Anderson constitutive promoter
IGEM
Lv0 Promoter p16 ABpSEVA182 J23106_ABJ23106 Anderson constitutive promoter
IGEM
Lv0 Promoter p28 ABpSEVA182 Pt7-LacO_ABPt7-LacO promoter repressed by LacI
Lv0 Promoter p30 ABpSEVA182 BG37_ABBG37 constitutive promoter from Zobel et al., 2015
Publication
Lv0 Promoter p31 ABpSEVA182 Pem7_ABPem7 constitutive promoter
Publication
Lv0 Promoter p33 ABpSEVA182 BG17_ABBG17 constitutive promoter from Zobel et al., 2015
Publication
Lv0 Promoter p40 ABpSEVA181 PrhaBAD_ABPrhaBAD promoter activated by RhaRS protein and L-rhamnose
Lv0 Promoter p49 ABpSEVA182 Pm_ABPm promoter activated by XylS protein and 3-methylbenzoate
Lv0 RBS r03 BDpSEVA182 B0034_BDMonocistronic RBS
IGEM
Lv0 RBS r04 BDpSEVA182 BCD12_BDBicistronic RBS
IGEM
Lv0 RBS r05 BDpSEVA182 BCD2_BDBicistronic RBS
IGEM
Lv0 RBS r07 BDpSEVA182 RBS_Std_BDRBS consensus adapted from Nogales et al.
Publication
Lv0 RBS r08 BDpSEVA182 T7RBS_BDRBS from pET plasmid
Lv0 RBS r12 BCpSEVA182 T7RBS_BCRBS from pET plasmid
C-tag1 ta02 FGpSEVA182 C-6xHistag_FGC-6xHistag
N-tag1 ta03 CDpSEVA182 N-6xHistag_CDN-6x Histidine tag
N-tag1 ta04 CDpSEVA182 N-Streptag_CDN-Streptavidine tag
C-tag1 ta05 FGpSEVA182 C-Streptag_FGC-Streptag
N-tag1 ta06 CDMA-T N-GST HRV3C tag_CDN-GST HRV3C protease site tag
N-tag1 ta07 CDMA-T N-MBP tag_CDN-MBP tag
C-tag1 ta08 FGpSEVA182 C-LinkGFP_FGC-LinkGFP (with STOP)
N-tag1 ta09 CDpSEVA182 N-OmpA teg_CDN-OmpA tag
N-tag1 ta10 CDpSEVA182 N-PelB_CDN-PelB
C-tag2 ta34 GHpSEVA181 C-Streptag_GHC- Streptavidine tag
C-tag1 ta36 FGpSEVA182 C-GFP_FGC-GFP (without STOP)
N-tag2 ta37 DEpSEVA182 N-mCherry_DEN-mCherry link
Lv0 Terminator te02 GIpICH rnpB_T1_GIT1 terminator from rnpB gene of E. coli MG1655
Lv0 Terminator te03 GIpICH rpoC_GITerminator from rpoBC operon of E. coli
Lv0 Terminator te04 GIpICH T500_GIT500 Terminator
Lv0 Terminator te06 GIpSEVA182 BBa_B1006_GIBBa_B1006 Artificial terminator
IGEM
Lv0 Terminator te10 GIpSEVA181 BBa_B0014_GIBBa_B0014 Artificial terminator
IGEM
Lv0 Terminator te11 HIpSEVA182 BBa_B1006_HIBBa_B1006 Artificial terminator
IGEM
Lv0 Host vector v0 pSEVA182Lv0 part host
Lv1 Host vector v01 1AI2 pSEVA23g19[g1]Lv1 host vector - 1AI2 cargo
Lv1 Host vector v02 2AI3 pSEVA23g19[g2]Lv1 host vector - 2AI3 cargo
Lv1 Host vector v03 3AI4 pSEVA23g19[g3]Lv1 host vector - 3AI4 cargo
Lv1 Host vector v04 4AI5 pSEVA23g19[g4]Lv1 host vector - 4AI5 cargo
Lv1 Host vector v05 5AI6 pSEVA23g19[g5]Lv1 host vector - 5AI6 cargo
Lv1 Host vector v06 6AI7 pSEVA23g19[g6]Lv1 host vector - 6AI7 cargo
Lv2 Host vector v07 A13B pSEVA63g19[gA]Lv2 host vector - A13B cargo
Lv2 Host vector v08 B14C pSEVA63g19[gB]Lv2 host vector - B14C cargo
Lv2 Host vector v09 C15D pSEVA63g19[gC]Lv2 host vector - C15D cargo
Lv2 Host vector v10 D16E pSEVA63g19[gD]Lv2 host vector - D16E cargo
Lv2 Host vector v11 E17F pSEVA63g19[gE]Lv2 host vector - E17F cargo
Lv3 Host vector v12 2AD3 pSEVA23g19[gβ]Lv3 host vector - 2AD3 cargo
Lv1 Host vector v13 1AI2 pSEVA2219[g1]Lv1 host vector - 1AI2 cargo
Lv2 Host vector v14 A13B pSEVA6819[gA]Lv2 host vector - A13B cargo
Lv1 Host vector v15 1AI2 pSEVA2819[g1]Lv1 host vector - 1AI2 cargo
Lv1 Host vector v16 2AI3 pSEVA2819[g2]Lv1 host vector - 2AI3 cargo
Lv1 Host vector v17 3AI4 pSEVA2819[g3]Lv1 host vector - 3AI4 cargo
Lv1 Host vector v18 4AI5 pSEVA2819[g4]Lv1 host vector - 4AI5 cargo
Lv1 Host vector v19 5AI6 pSEVA2819[g5]Lv1 host vector - 5AI6 cargo
Lv1 Host vector v20 6AI7 pSEVA2819[g6]Lv1 host vector - 6AI7 cargo
Lv2 Host vector v21 A13B pSEVA6219[gA]Lv2 host vector - A13B cargo
Lv3 Host vector v22 1AC2 pSEVA23g19[gα]Lv3 host vector - 1AC2 cargo
Lv1 Host vector v23 2AI3 pSEVA2219[g2]Lv1 host vector - 2AI3 cargo
Lv2 Host vector v26 B14C pSEVA6819[gB]Lv2 host vector - B14C cargo
Lv2 Host vector v28 C15D pSEVA6219[gC]Lv2 host vector - C15D cargo
Lv2 Host vector v31 C15D pSEVA6819[gC]Lv2 host vector - C15D cargo
Lv2 Host vector v32 D16E pSEVA6819[gD]Lv2 host vector - D16E cargo
Lv2 Host vector v33 E17F pSEVA6819[gE]Lv2 host vector - E17F cargo
Lv1 Host vector v34 1IA2 pSEVA23g19[g1R]Lv1 host vector - 1IA2 cargo
Lv1 Host vector v35 1IA2 pSEVA2819[g1R]Lv1 host vector - 1IA2 cargo
Lv1 Host vector v36 3AI4 pSEVA2219[g3]Lv1 host vector - 3AI4 cargo
Lv1 Host vector v37 4AI5 pSEVA2219[g4]Lv1 host vector - 4AI5 cargo
Lv1 Host vector v38 5AI6 pSEVA2219[g5]Lv1 host vector - 5AI6 cargo
Lv1 Host vector v39 6AI7 pSEVA2219[g6]Lv1 host vector - 6AI7 cargo
Lv1 Host vector v40 1IA2 pSEVA2219[g1R]Lv1 host vector - 1IA2 cargo
Lv2 Host vector v41 B14C pSEVA6219[gB]Lv2 host vector - B14C cargo
Lv2 Host vector v42 D16E pSEVA6219[gD]Lv2 host vector - D16E cargo
Lv2 Host vector v43 E17F pSEVA6219[gE]Lv2 host vector - E17F cargo
Lv3 Host vector v44 3AE4 pSEVA23g19[gγ]Lv3 host vector - 3AE4 cargo
Lv3 Host vector v45 4AF5 pSEVA23g19[gẟ]Lv3 host vector - 4AF5 cargo

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